Comparing the effectiveness of R genes in a 2-year canola–wheat rotation against Leptosphaeria maculans, the causal agent of blackleg disease in Brassica species

Rashid, MH; Liban, Sakaria; Zhang, Xuehua; Parks, Paula; Borhan, M. Hossein; Fernando, W. G. Dilantha

doi:10.1007/s10658-022-02498-7

Cited by 3 publications

(2 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…LepR1 can also be an significant source of blackleg resistance for the cultivars in Australia, which has virulent L. maculans population causing the breakdown of the cultivars containing R gene like LepR3 and ineffectiveness of R genes like Rlm1 and Rlm3 (Li et al, 2005;Van de Wouw et al, 2014;Van de Wouw et al, 2021). Other studies have also shown that cultivars with LepR1 are more effective than those with R genes Rlm1, Rlm3, Rlm4, and LepR3 (Alnajar et al, 2022;Rashid et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Identification of candidate genes for LepR1 resistance against Leptosphaeria maculans in Brassica napus

Cantila

Thomas²,

Saad³

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

Utilising resistance (R) genes, such as LepR1, against Leptosphaeria maculans, the causal agent of blackleg in canola (Brassica napus), could help manage the disease in the field and increase crop yield. Here we present a genome wide association study (GWAS) in B. napus to identify LepR1 candidate genes. Disease phenotyping of 104 B. napus genotypes revealed 30 resistant and 74 susceptible lines. Whole genome re-sequencing of these cultivars yielded over 3 million high quality single nucleotide polymorphisms (SNPs). GWAS in mixed linear model (MLM) revealed a total of 2,166 significant SNPs associated with LepR1 resistance. Of these SNPs, 2108 (97%) were found on chromosome A02 of B. napus cv. Darmor bzh v9 with a delineated LepR1_mlm1 QTL at 15.11-26.08 Mb. In LepR1_mlm1, there are 30 resistance gene analogs (RGAs) (13 nucleotide-binding site-leucine rich repeats (NLRs), 12 receptor-like kinases (RLKs), and 5 transmembrane-coiled-coil (TM-CCs)). Sequence analysis of alleles in resistant and susceptible lines was undertaken to identify candidate genes. This research provides insights into blackleg resistance in B. napus and assists identification of the functional LepR1 blackleg resistance gene.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification of candidate genes for LepR1 resistance against Leptosphaeria maculans in Brassica napus

Cantila

Thomas²,

Saad³

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…Several other nations have also begun investing in oilseed enhancement programs. For example, the Canola Council of Canada has projected an overall increase in B. napus yield from ~21 Mt in 2020 to ~25 Mt in 2025 as per their enhancement programs to meet global demand [ 18 , 19 ]. Furthermore, the Australian Field Applied Research (AFAR) initiative showed that Australia is capable of producing B. napus yields in excess of 6 t/ha [ 20 ] nearly doubling Europe’s efficiency.…”

Section: Introductionmentioning

confidence: 99%

The Global Assessment of Oilseed Brassica Crop Species Yield, Yield Stability and the Underlying Genetics

Zandberg

Fernandez

Danilevicz

et al. 2022

Plants

View full text Add to dashboard Cite

The global demand for oilseeds is increasing along with the human population. The family of Brassicaceae crops are no exception, typically harvested as a valuable source of oil, rich in beneficial molecules important for human health. The global capacity for improving Brassica yield has steadily risen over the last 50 years, with the major crop Brassica napus (rapeseed, canola) production increasing to ~72 Gt in 2020. In contrast, the production of Brassica mustard crops has fluctuated, rarely improving in farming efficiency. The drastic increase in global yield of B. napus is largely due to the demand for a stable source of cooking oil. Furthermore, with the adoption of highly efficient farming techniques, yield enhancement programs, breeding programs, the integration of high-throughput phenotyping technology and establishing the underlying genetics, B. napus yields have increased by >450 fold since 1978. Yield stability has been improved with new management strategies targeting diseases and pests, as well as by understanding the complex interaction of environment, phenotype and genotype. This review assesses the global yield and yield stability of agriculturally important oilseed Brassica species and discusses how contemporary farming and genetic techniques have driven improvements.

show abstract

Strategic genetic insights and integrated approaches for successful management of blackleg in canola/rapeseed farming

Rouxel,

Peng,

Van de Wouw

et al. 2024

Plant Pathology

View full text Add to dashboard Cite

This review describes a triumphant narrative in the battle against the devastating plant pathogen complex, Leptosphaeria maculans and L. biglobosa, and the success of the world's second‐largest oilseed crop, canola/oilseed rape. Emphasizing global collaborations, the article explores successfully mitigating this destructive disease in canola/rapeseed production across Australia, Canada and Europe. It highlights how strategies may vary between continents to adapt to specific contexts. While initial resistance (R) genes proved effective, the evolution of the pathogen under crop‐induced disease pressure led to the breakdown of these genes. Now, growers in these regions have been equipped with new tools, allowing them to make informed decisions that help to keep the disease at generally low levels. A pivotal factor in this success has been a deepened understanding of the intricate science underlying the host–pathogen interaction. Concerted efforts of individual laboratories and collaborative initiatives have played an essential role in this success, including novel methods for disease control based on extensive research that has translated into developing highly resistant varieties, enhanced pathogen monitoring, improved cultivar recommendation and integrated management strategies. The review showcases many milestone advancements, including the cloning of numerous avirulence genes within the pathogen, characterization of specific R genes, the development of various molecular tools for monitoring both pathogen and host, the introduction of groundbreaking disease management strategies such as R gene labelling, rotation and stacking, and establishment of a universal pathogen isolate collection that facilitates the exchange of information among multiple laboratories and adds a new dimension to this triumph.

show abstract

Comparing the effectiveness of R genes in a 2-year canola–wheat rotation against Leptosphaeria maculans, the causal agent of blackleg disease in Brassica species

Cited by 3 publications

References 43 publications

Identification of candidate genes for LepR1 resistance against Leptosphaeria maculans in Brassica napus

Identification of candidate genes for LepR1 resistance against Leptosphaeria maculans in Brassica napus

The Global Assessment of Oilseed Brassica Crop Species Yield, Yield Stability and the Underlying Genetics

Strategic genetic insights and integrated approaches for successful management of blackleg in canola/rapeseed farming

Contact Info

Product

Resources

About