Comparing the sensitivity of different intestinal Caco-2 in vitro monocultures and co-cultures to amorphous silicon dioxide nanomaterials and the clay montmorillonite

Ude, Victor Chibueze; Brown, David M.; Maciaszek, Krystyna; Stone, Vicki; Johnston, Helinor Jane

doi:10.1016/j.impact.2019.100165

Cited by 8 publications

(3 citation statements)

References 66 publications

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“…Mirroring the SiO 2 induced upregulation of IL-8 we observed in the stable triple culture, Ude et al demonstrated the secretion of IL-8 from Caco-2/E12 cell co-culture exposed to 15.63 µg/cm 2 SiO 2 for 24 h [72]. In contrast, Ag exposure did not increase the IL-8 gene expression in both triple culture models, which aligns with previous findings on the absence of an Ag induced IL-8 secretion from these models [52].…”

Section: Enm and Model Dependent Effects On Il-8 Expressionmentioning

confidence: 64%

“…In addition to pre-confluent and confluent E12 cells in monoculture, we evaluated ENM effects on triple cultures of Caco-2, E12 and differentiated THP-1 cells, both in stable and inflamed states. Co-cultures including Caco-2 and HT29-MTX(-E12) cells are frequently used as in vitro models of the mucus-covered intestinal epithelium for toxicity testing [17,27,52,72,91]. Yet, little research is available on the response of HT29-MTX(-E12) cells to ENM exposure.…”

Section: Model Comparisonsmentioning

confidence: 99%

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Ingested Engineered Nanomaterials Affect the Expression of Mucin Genes—An In Vitro-In Vivo Comparison

et al. 2021

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The increasing use of engineered nanomaterials (ENM) in food has fueled the development of intestinal in vitro models for toxicity testing. However, ENM effects on intestinal mucus have barely been addressed, although its crucial role for intestinal health is evident. We investigated the effects of ENM on mucin expression and aimed to evaluate the suitability of four in vitro models of increasing complexity compared to a mouse model exposed through feed pellets. We assessed the gene expression of the mucins MUC1, MUC2, MUC5AC, MUC13 and MUC20 and the chemokine interleukin-8 in pre-confluent and confluent HT29-MTX-E12 cells, in stable and inflamed triple cultures of Caco-2, HT29-MTX-E12 and THP-1 cells, and in the ileum of mice following exposure to TiO2, Ag, CeO2 or SiO2. All ENM had shared and specific effects. CeO2 downregulated MUC1 in confluent E12 cells and in mice. Ag induced downregulation of Muc2 in mice. Overall, the in vivo data were consistent with the findings in the stable triple cultures and the confluent HT29-MTX-E12 cells but not in pre-confluent cells, indicating the higher relevance of advanced models for hazard assessment. The effects on MUC1 and MUC2 suggest that specific ENM may lead to an elevated susceptibility towards intestinal infections and inflammations.

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Section: Enm and Model Dependent Effects On Il-8 Expressionmentioning

confidence: 64%

Section: Model Comparisonsmentioning

confidence: 99%

Ingested Engineered Nanomaterials Affect the Expression of Mucin Genes—An In Vitro-In Vivo Comparison

et al. 2021

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“…Addition of lymphocytes imparts Caco-2 cell lines (monolayers) with M cell-mimicking capacities 126 . This is commonly done by co-culturing Caco-2 cells with lymphoid cells like Raji cells or murine derived lymphocytes 248 , 249 . Among different culture protocols, filter-grown co-culture Caco-2/Raji system was the most investigated one 250 .…”

Section: Models For Evaluation Of Lymphatic Transportmentioning

confidence: 99%

An update on oral drug delivery via intestinal lymphatic transport

Zi-chen

Lü

et al. 2021

Acta Pharmaceutica Sinica B

118

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Orally administered drug entities have to survive the harsh gastrointestinal environment, penetrate the enteric epithelia and circumvent hepatic metabolism before reaching the systemic circulation. Whereas the gastrointestinal stability can be well maintained by taking proper measures, hepatic metabolism presents as a formidable barrier to drugs suffering from first-pass metabolism. The pharmaceutical academia and industries are seeking alternative pathways for drug transport to circumvent problems associated with the portal pathway. Intestinal lymphatic transport is emerging as a promising pathway to this end. In this review, we intend to provide an updated overview on the rationale, strategies, factors and applications involved in intestinal lymphatic transport. There are mainly two pathways for peroral lymphatic transport—the chylomicron and the microfold cell pathways. The underlying mechanisms are being unraveled gradually and nowadays witness increasing research input and applications.

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Predictive models for nanotoxicology: in vitro, in vivo, and computational models

Pimtong

Samutrtai

Wongwanakul

et al. 2021

Handbook of Nanotechnology Applications

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Comparing the sensitivity of different intestinal Caco-2 in vitro monocultures and co-cultures to amorphous silicon dioxide nanomaterials and the clay montmorillonite

Cited by 8 publications

References 66 publications

Ingested Engineered Nanomaterials Affect the Expression of Mucin Genes—An In Vitro-In Vivo Comparison

Ingested Engineered Nanomaterials Affect the Expression of Mucin Genes—An In Vitro-In Vivo Comparison

An update on oral drug delivery via intestinal lymphatic transport

Predictive models for nanotoxicology: in vitro, in vivo, and computational models

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