Comparison: Adsorption of papain using immobilized dye ligands on affinity membranes

Dye Affinity Nanofiber Membrane for Adsorption of Lysozyme: Preparation and Performance Evaluation

“…9, it was observed that the binding capacity was increased from 154.3 to 309.2 mg/g as the temperature increased from 277 to 323 K. An increase in temperature caused an increase in binding capacity. Similar results were also observed in the adsorption of lysozyme by other dyed membranes (27,(39)(40)(41)(42)(43). The increase in binding capacity of the membrane was related to the conformational changes of the lysozyme molecule.…”

“…The increase in binding capacity of the membrane was related to the conformational changes of the lysozyme molecule. Moreover, this could be attributed to an increase in the hydrophobic interaction as the temperature increased (41). …”

Constituting of a new surface‐initiating system on polymeric microspheres and preparation of basic protein surface‐imprinted material in aqueous solution

“…Papain is a basic protein (pI = 8.7), and this protease has high biological activity and heat stability. It is widely used in the leather, cosmetic, textiles, detergents, food and pharmaceutical industries . Therefore, there is an increased requirement for papain in industry.…”

Usage of immobilized papain for enzymatic hydrolysis of proteins

“…Papain has been used in cell isolation studies and in the separation of biomolecules in large-scale industry processes. Besides, papain is widely used in the leather, cosmetic, textiles, detergents, food, and pharmaceutical industries [4].…”