Comparison among bone marrow mesenchymal stem and mononuclear cells to promote functional recovery after spinal cord injury in rabbits

Abstract

Comparison among bone marrow mesenchymal stem and mononuclear cells to promote functional recovery after spinal cord injury in rabbits 1 Abstract Purpose: To investigate the efficacy of allogeneic mesenchymal stem-cells and autologous mononuclear cells to promote sensorimotor recovery and tissue rescue. Methods: Female rabbits were submitted to the epidural balloon inflation method and the intravenous cells administrations were made after 8 hours or seven days after injury induction. Sensorimotor evaluation of the hindlimbs was performed, and the euthanasia was made thirty days after the treatment. Spinal cords were stained with hematoxylin and eosin. Results: Both therapies given 8 hours after the injury promoted the sensorimotor recovery after a week. Only the group treated after a week with mononuclear cells showed no significant recovery at post-injury day 14. In the days 21 and 28, all treatments promoted significant recovery. Histopathological analysis showed no difference among the experimental groups. Our results showed that both bone marrow-derived cell types promoted significant sensorimotor recovery after injury, and the treatment made at least a week after injury is efficient. Conclusion:The possibilities of therapy with bone marrow-derived cells are large, increasing the therapeutic arsenal for the treatment of spinal cord injury.

Laser-activated autologous adipose tissue-derived stromal vascular fraction restores spinal cord architecture and function in multiple sclerosis cat model

“…Otherwise, recent medications are limited to controlling immune responses that may only be useful in the early stages of the disease [ 5 , 7 ]. Rehabilitation that depends on the use of regenerative stem cell medicines has generated a new promise in supporting spinal cord function–structural recovery [ 1 , 8 ] in dogs [ 9 , 10 ], monkeys [ 11 , 12 ], rats [ 13 , 14 ], and rabbits [ 15 , 16 ]. Mesenchymal stem cells (MSCs) can be harvested and processed easily from adipose tissues with minimal invasion and infection, giving rise to oligodendrocytes and neural progenitor cells [ 2 , 17 ].…”

Human Adipose-Derived Stem Cells Reduce Cellular Damage after Experimental Spinal Cord Injury in Rats

“…Other options of treatment are corticoids, riluzole, hormones, calcium channel blockers, and specific proteins, which are ineffective in the treatment of SCI [8,9]. The administration of stem cells (SC) presents as a promising strategy since it can potentially interrupt or minimize the cascade of pathophysiological events and provide a favorable environment for specialized tissue regeneration, with possible positive clinical correlation [10][11][12]. Adult stem cells, such as mesenchymal stem cells (MSC), are often used in clinical research for their immunomodulatory and regenerative potential [9,13].…”