Comparison among different cryoprotectants for cryopreservation of epididymal sperm from agouti (Dasyprocta leporina)

Castelo, T.S.; Silva, Alexandre Rodrigues; Bezerra, Luana Grasiele Pereira; Costa, Clara Y. M.; Lago, Arthur Emannuel de Araújo; Bezerra, José Artur Brilhante; Campos, Lívia Batista; Praxedes, Érica Camila Gurgel

doi:10.1016/j.cryobiol.2015.09.005

Cited by 22 publications

(13 citation statements)

References 39 publications

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“…In recent decades, various efforts have been made to establish protocols to safeguard germplasm derived from valuable wild individuals that suddenly die, such as the Cantabrian brown bear (Anel et al ., 2011), the agouti (Castelo et al ., 2015), the red deer (García-Álvarez et al ., 2016), and the dromedary camel (Scholkamy et al ., 2016). To the best of our knowledge, this is the first report on the cryopreservation of epididymal sperm of collared peccaries.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of collared peccary (Pecari tajacu L., 1758) epididymal sperm using extenders based on Tris and powdered coconut water (ACP®-116c)

Bezerra

Silva

Sousa

et al. 2018

Zygote

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SummaryThe aim of this study was to establish a functional freezing-thawing protocol for epididymal sperm of collared peccaries (Pecari tajacu L., 1758) by comparing different extenders. The epididymal sperm from 12 sexually mature males was recovered by retrograde flushing using Tris-based or coconut water-based (ACP®-116c) extenders. After initial evaluation, samples were diluted and frozen with the same extenders to which 20% egg yolk and 6% glycerol were added. After 2 weeks, thawing was performed at 37°C/60 s and sperm motility, vigour, morphology, functional membrane integrity, sperm viability, sperm plasma membrane integrity, and a computer-assisted semen analysis (CASA) were assessed. In addition, to evaluate the survival of frozen-thawed sperm, a thermal resistance test (TRT) was executed. Samples preserved using Tris were in better condition compared with those preserved using ACP®, showing higher values for most assessments performed, including CASA and the TRT (P<0.05). After determining Tris to be the better of the two extenders, additional samples were thawed using different thawing rates (37°C/60 s, 55°C/7 s, 70°C/8 s). Sperm thawed at 37°C/60 s had the greatest preservation (P<0.05) of viability (54.1 ± 5.9%) and functional membrane integrity (43.2 ± 5.4%), and had higher values for various CASA parameters. In conclusion, we suggest the use of a Tris-based extender added to egg yolk and glycerol for the cryopreservation of epididymal sperm obtained from collared peccaries. In order to achieve better post-thawing sperm quality, we suggest that samples should be thawed at 37°C/60 s.

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Section: Discussionmentioning

confidence: 99%

Cryopreservation of collared peccary (Pecari tajacu L., 1758) epididymal sperm using extenders based on Tris and powdered coconut water (ACP®-116c)

Bezerra

Silva

Sousa

et al. 2018

Zygote

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“…1478912) were used in this study. The animals were previously anesthetized by intramuscular administration of 15mg/kg ketamine hydrochloride (Ketalar; Pfizer, São Paulo/SP, Brazil) and 1mg/kg xylazine hydrochloride (Rompun; Bayer, São Paulo/SP, Brazil), according to Castelo et al (2015). Ear margin tissues (1-2cm 2 ) were obtained using pliers and transported to the laboratory in Dulbecco's modified Eagle's medium (DMEM) supplemented with 2% antibiotic-antimycotic solution (penicillin G, streptomycin, and amphotericin B) at 37°C for 1 h.…”

Section: Methodsmentioning

confidence: 99%

Ultrastructural and morphometric description of the ear skin and cartilage of two South American wild histricognate rodents (Dasyprocta leporina and Galea spixii)

et al. 2021

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Skin and cartilage have been the main source for the recovery of somatic cells to be used in conservation strategies in wild mammals. In this sense, an important step for the cryopreservation of these samples is to recognize the properties of the skin and cartilage. Thus, knowing that the skin may differ among species and aiming to contribute to the establishment of cryobanks, the study examined the differences in the ear skin and cartilage of wild rodents from South America, agouti (Dasyprocta leporina) and spix’s yellow-toothed cavy (Galea spixii). Ultrastructural and quantitative methods were used to measure skin and cartilage thickness, density of collagen and elastic fibers, cell type number and distribution, and proliferative activity. Although ultrastructural analysis revealed a similar pattern between species, morphometric analysis of the skin and cartilage showed differences between agoutis and cavies regarding thickness of epidermis layers (corneum: 5.3±2.5μm vs. 3.9±0.6μm; intermediate: 16.4±6.2μm vs. 23.4±8.1μm; basal: 9.9±2.1μm vs. 4.8±0.5μm), dermis (183.1±44.0μm vs. 258.2±22.9μm), total skin (211.8±46.0μm vs. 290.3±23.7μm) and perichondrium (27.6±6.1μm vs. 10.5±1.8μm). A greater number of epidermal cells (61.7±15.2 vs. 24.8±7.6) and chondrocytes (32.7±9.0 vs. 27.5±4.7) were observed in agouti, while the cavy presented a greater number of melanocytes (12.6±4.7 vs. 29.9±6.2), keratinocytes (14.7±4.2 vs. 29.8±7.6), and fibroblasts (103.6±24.7 vs. 112.2±11.3). Moreover, a higher percentage of collagen fibers and proliferative activity was observed in the skin of cavies, when compared to the skin of agoutis. Therefore, there are differences between agouti and cavy for ear skin and cartilage, requiring the establishment of species-specific cryopreservation protocols.

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“…To reduce or prevent the injuries caused by low temperature, it is essential to add substances that provide the sperm with cryoprotection. The efficiency of cryoprotectant agents may vary according to the structure of the cryopreserved cell or tissue, as well as the concentration of cryoprotectant agents and exposure time used before the cryopreservation process (Castelo et al, 2015). Glycerol is the most widely used cryoprotectant for ram sperm because it decreases the mechanical damage to sperm during the freezing process (Najafi et al, 2013;Yang et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Ethylene glycol, but not DMSO, could replace glycerol inclusion in soybean lecithin-based extenders in ram sperm cryopreservation

Najafi

Kia

Dodaran

et al. 2017

Animal Reproduction Science

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Comparison among different cryoprotectants for cryopreservation of epididymal sperm from agouti (Dasyprocta leporina)

Cited by 22 publications

References 39 publications

Cryopreservation of collared peccary (Pecari tajacu L., 1758) epididymal sperm using extenders based on Tris and powdered coconut water (ACP®-116c)

Cryopreservation of collared peccary (Pecari tajacu L., 1758) epididymal sperm using extenders based on Tris and powdered coconut water (ACP®-116c)

Ultrastructural and morphometric description of the ear skin and cartilage of two South American wild histricognate rodents (Dasyprocta leporina and Galea spixii)

Ethylene glycol, but not DMSO, could replace glycerol inclusion in soybean lecithin-based extenders in ram sperm cryopreservation

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