Comparison and correlation study of synovial ultrasound indices and serum VEGF in rheumatoid wrist arthritis before and after treatment

Zhang, Yanfen; Gao, Shanshan; Li, Jiali; Zuo, Wen-si; Qiu, Yue-wen; Xiao, Yao-cheng

doi:10.1007/s10067-022-06213-z

Cited by 3 publications

(2 citation statements)

References 28 publications

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“…In this regard, power Doppler vascularity was positively linked with the presence of inflammatory cytokines and growth factors in synovial fluid, with larger coefficient values in both untreated and treated individuals with RA. There is also a significant relationship between the findings of the US synovial examination and the levels of serum vascular endothelial growth factor (VEGF) [45], YKL-40 [46], and calprotectin [47] in RA patients.…”

Section: Discussionmentioning

confidence: 99%

TNF-α in the serum and synovial fluid of patients with rheumatoid arthritis: correlation with sonographic parameters: a cross-sectional study

Hassany,

Tharwat,

Mansour

et al. 2024

Egypt Rheumatol Rehabil

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Background Rheumatoid arthritis (RA) is a chronic, inflammatory joint condition characterized by overproduction of pro-inflammatory cytokines. We aimed to assess TNF-α levels in both serum and synovial fluid in effusive knees in RA patients and find out if synovial fluid levels correlate with ultrasound (US)-detected local knee inflammatory and/or destructive changes in these patients. Results This study included 40 patients (20 with RA, 10 with systemic lupus erythematosus (SLE), and 10 with osteoarthritis (OA)) who had knee effusion (unilateral or bilateral) upon clinical examination. The mean age of RA patients was 48.4 years; most of them were females (80%), with a median (min–max) duration of knee effusion of 2 (1.5–3) months. Serum TNF-α was significantly higher in RA vs. non-RA and in OA cases (p = 0.052, 0.022, respectively), while in the synovial fluid, the difference was not statistically significant (3.73 ± 0.72 vs. 3.48 ± 0.58 U/ml, p = 0.252). Serum TNF-α at a cut point of > 3.24 U/ml can significantly discriminate RA from OA with 65% sensitivity and 90% specificity (AUC = 0.725, P = 0.018). There was no statistically significant correlation between synovial TNF-α and US parameters of the knee, either in RA or non-RA patients. Conclusions RA, OA, and SLE effusive joints share the presence of local articular joint inflammation, while systemic inflammation is more discriminative for RA patients regarding the level of TNF-α. The lack of correlation of TNF-α with ultrasonographic findings reflects the multifactorial complexity of these autoimmune diseases.

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Section: Discussionmentioning

confidence: 99%

TNF-α in the serum and synovial fluid of patients with rheumatoid arthritis: correlation with sonographic parameters: a cross-sectional study

Hassany,

Tharwat,

Mansour

et al. 2024

Egypt Rheumatol Rehabil

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“…Studies have shown that VEGF gene polymorphisms are associated with RA susceptibility and activity and can be used for the clinical diagnosis and treatment of RA. High expression of VEGF can increase small vessel density in synovial inflammatory areas and elevate the levels of inflammatory factors, such as TNF-a and IL-1b (205)(206)(207).…”

Section: Role Of Ros and Mitochondrial Damage In Ra Angiogenesismentioning

confidence: 99%

Role of reactive oxygen species and mitochondrial damage in rheumatoid arthritis and targeted drugs

et al. 2023

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Rheumatoid arthritis (RA) is an autoimmune disease characterized by synovial inflammation, pannus formation, and bone and cartilage damage. It has a high disability rate. The hypoxic microenvironment of RA joints can cause reactive oxygen species (ROS) accumulation and mitochondrial damage, which not only affect the metabolic processes of immune cells and pathological changes in fibroblastic synovial cells but also upregulate the expression of several inflammatory pathways, ultimately promoting inflammation. Additionally, ROS and mitochondrial damage are involved in angiogenesis and bone destruction, thereby accelerating RA progression. In this review, we highlighted the effects of ROS accumulation and mitochondrial damage on inflammatory response, angiogenesis, bone and cartilage damage in RA. Additionally, we summarized therapies that target ROS or mitochondria to relieve RA symptoms and discuss the gaps in research and existing controversies, hoping to provide new ideas for research in this area and insights for targeted drug development in RA.

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The Effect and Mechanism of External Use Ulcer Powder on Diabetic Mice

Wang¹,

Xie²,

Lu³

et al. 2022

Cureus

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Through the preparation of the diabetic mice skin ulcer model, we investigated the effect of Mongolian medicine external ulcer powder (WYK) on the treatment of diabetic skin ulcers and the expression of angiogenesis-related factors such as vascular endothelial growth factor (VEGF) and extracellular regulated protein kinases (ERK). MethodsThirty male clean Kunming mice were randomly divided into normal control group (group C), diabetic control group (group HC), and diabetic topical ulcer powder group (group HW). After successful modeling in the HC group and the HW group, the rats in the HW group were given external ulcer powder, which was applied to the back of the mice once a day. In addition, the rats in group C and group HC were treated with gentamicin injection external application once a day. The mice were sacrificed on the 3rd, the sixth, and the ninth day of dosing, and samples were taken. The adopted methods included protein immunoblotting (western blot) and reverse transcription-polymerase chain reaction (RT-PCR). The expression differences of angiogenesis-related factors such as VEGF and ERK in the repair process were detected. SPSS 13 software was used to analyze the results of angiogenesis-related factors VEGF and ERK. Results Comparison of VEGF and ERK ContentsThe serum VEGF content of mice in the HC group was significantly lower than that in the C group on days 3, 6, and 9 (p <0.05). The VEGF content in the HW group was significantly higher than that in the HC group (p <0.05). The content of ERK in serum was basically consistent with that of VEGF. The results of the western blot assay were consistent with those of the RT-PCR assay. ConclusionWYK can effectively promote the healing of skin ulcer wounds in diabetic mice, accelerate the proliferation of granulation tissue, enrich the contents of capillary blood tubes and collagen fibers, and increase the microvascular content. WYK can improve the expression level of VEGF and ERK in the serum of mice and advance the peak value of protein expression.

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Comparison and correlation study of synovial ultrasound indices and serum VEGF in rheumatoid wrist arthritis before and after treatment

Cited by 3 publications

References 28 publications

TNF-α in the serum and synovial fluid of patients with rheumatoid arthritis: correlation with sonographic parameters: a cross-sectional study

TNF-α in the serum and synovial fluid of patients with rheumatoid arthritis: correlation with sonographic parameters: a cross-sectional study

Role of reactive oxygen species and mitochondrial damage in rheumatoid arthritis and targeted drugs

The Effect and Mechanism of External Use Ulcer Powder on Diabetic Mice

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