Comparison and functional characterisation of peripheral blood mononuclear cells isolated from filarial lymphoedema and endemic normals of a South Indian population

Nathan, Abel Arul; Dixit, Madhulika; Babu, Subash; Balakrishnan, Anand

doi:10.1111/tmi.12969

Cited by 1 publication

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References 49 publications

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“…Like IL-9, the basal level of SDF-1a was found to be significantly high in LF+ subjects with diabetes. Previously, SDF-1/CXCR4 was found to promote chronic pathology in LF+ subjects (50). Thus, diabetes, by augmenting IL-9 and SDF-1, can promote chronic pathology in LF+ subjects.…”

Section: Discussionmentioning

confidence: 89%

Augmented Innate and Adaptive Immune Responses Under Conditions of Diabetes–Filariasis Comorbidity

et al. 2021

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Metainflammation, as seen in chronic diabetes subjects, impairs immunity and increases the susceptibility to infections. In the present study, the effect of diabetes on immune response against filariasis was studied. Both toll-like receptor (TLR)-mediated and crude antigen-induced immune responses were quantified, in whole blood cultures from filariasis-infected subjects (LF+), with and without diabetes. Blood cultures were stimulated with TLR ligands (TLR2 and TLR4) or filarial antigen or were left unstimulated (control) for 18 h. Cytokine, chemokine, and defensin secretion was quantified by ELISA. Expression of HLA-DR, B7-1, B7-2, activation marker (CD69), and Th (Th1, Th2, Th17, and Th9) phenotypes was quantified by flow cytometry. Expression of immunomodulatory effectors (Cox-2, HO-1, IDO-1, and p47Phox) and Th-polarizing transcription factors (T-bet, GATA3, and ROR-γt) was quantified by quantitative PCR. Secretion of IL-27, IL-1Ra, IL-12, IL-33, IL-9, and SDF-1 was increased under diabetes conditions with increased Th9 polarization and increased expression of Cox-2 and IDO. Overall, diabetes was found to augment both TLR-mediated and antigen-induced inflammation, which can promote chronic pathology in LF+ subjects.

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Section: Discussionmentioning

confidence: 89%

Augmented Innate and Adaptive Immune Responses Under Conditions of Diabetes–Filariasis Comorbidity

et al. 2021

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Comparison and functional characterisation of peripheral blood mononuclear cells isolated from filarial lymphoedema and endemic normals of a South Indian population

Abstract: PBMCs from filarial lymphoedema show defective and dysregulated lymphvasculogenic function compared to endemic normals.

Cited by 1 publication

References 49 publications

Augmented Innate and Adaptive Immune Responses Under Conditions of Diabetes–Filariasis Comorbidity

Augmented Innate and Adaptive Immune Responses Under Conditions of Diabetes–Filariasis Comorbidity

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