Comparison between culture of bovine embryos in vitro versus development in rabbit oviducts and in vivo

Ectors, Fabien; Thonon, F.; Delval, A.; Fontes, R. S.; Touati, Kamal; Beckers, J. F.

doi:10.1016/0301-6226(93)90134-4

Cited by 13 publications

(7 citation statements)

References 14 publications

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“…FBS, above certain concentrations (>5% v/v), may improve embryo production, but it has been associated with compromising cryotolerance and inducing LOS (Lazzari et al, 2002). When bovine zygotes were cultured in the ewe (Lazzari et al, 2010), rabbit (Ectors et al, 1993), mouse (Rizos et al, 2010) or even the homologous bovine (Gad et al, 2012) oviduct, blastocyst quality, cryotolerance, embryonic genome activation and gene expression were similar to that of the in vivo-derived counterpart. Furthermore, the use of BOECs showed positive effects on embryo development and quality (Schmaltz-Panneau et al, 2015).…”

Section: Embryo Developmentmentioning

confidence: 98%

Review: Recent advances in bovine in vitro embryo production: reproductive biotechnology history and methods

Ferré¹,

Kjelland²,

Strøbech³

et al. 2020

Animal

183

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In vitro production (IVP) of embryos and associated technologies in cattle have shown significant progress in recent years, in part driven by a better understanding of the full potential of these tools by end users. The combination of IVP with sexed semen (SS) and genomic selection (GS) is being successfully and widely used in North America, South America and Europe. The main advantages offered by these technologies include a higher number of embryos and pregnancies per unit of time, and a wider range of potential female donors from which to retrieve oocytes (including open cyclic females and ones up to 3 months pregnant), including high index genomic calves, a reduced number of sperm required to produce embryos and increased chances of obtaining the desired sex of offspring. However, there are still unresolved aspects of IVP of embryos that limit a wider implementation of the technology, including potentially reduced fertility from the use of SS, reduced oocyte quality after in vitro oocyte maturation and lower embryo cryotolerance, resulting in reduced pregnancy rates compared to in vivo–produced embryos. Nevertheless, promising research results have been reported, and work is in progress to address current deficiencies. The combination of GS, IVP and SS has proven successful in the commercial field in several countries assisting practitioners and cattle producers to improve reproductive performance, efficiency and genetic gain.

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Section: Embryo Developmentmentioning

confidence: 98%

Review: Recent advances in bovine in vitro embryo production: reproductive biotechnology history and methods

Ferré¹,

Kjelland²,

Strøbech³

et al. 2020

Animal

183

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“…Crucial processes, including oocyte maturation, sperm capacitation, fertilization and early embryonic development occur in the oviduct (Hunter 1988;Leese 1988;Gandolfi et al 1989a;Ellington 1991). Oviducts from sheep (Eyestone et al 1987) and rabbits (Ellington et al 1990;Ectors et al 1993) have been used as a temporary in vivo culture system for in vitro produced (IVP) bovine embryos to overcome the eightcell developmental block (Bavister 1988). Bovine oviduct cell monolayers have been employed as an in vitro co-culture system for IVP bovine embryos (Thibodeaux et al 1992;Xu et al 1992;Myers et al 1994); however, the rates of embryonic development are still lower than in vivo.…”

Section: Introductionmentioning

confidence: 99%

Factors affecting proliferation and dedifferentiation of primary bovine oviduct epithelial cells in vitro

Reischl

Prelle

Schöl

et al. 1999

Cell and Tissue Research

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The oviduct is the physiological site for key events in reproduction, such as capacitation of spermatozoa, fertilization and early embryonic development. Interactions between oviduct epithelial cells and gametes or embryos cannot sufficiently be studied in vivo. Therefore, model systems are needed which mimic in vivo conditions most closely. In this study we optimised the method for isolating bovine oviduct cells and compared different cell support materials as well as two culture systems (perfusion vs static culture) for their ability to maintain characteristic morphological and functional features of oviduct cells. Out of nine different cell support materials tested, cellulose nitrate (0.45 micron pore size) was the most suitable to maintain cells in a manner similar to freshly isolated oviduct epithelial cells. Comparing static vs perfusion culture by electron microscopy, morphological differences of the cells were insignificant in the first days of culture, while they became more evident after 8 days. The cells in the static system lost typical characteristics such as columnar shape, cilia and secretory protrusions, while these features were still present in perfusion culture. In addition, intense ciliogenesis and cytoplasmic organelles for protein synthesis were found under perfusion conditions. These findings were underlined by differences in expression of the oviduct-specific oestrus-associated glycoprotein 85-97 kDa (GP 85-97) gene as revealed by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). The RNA levels of this specific gene were significantly higher in perfusion compared to the static culture system. Our data show clear advantages of perfusion vs static culture for primary bovine oviduct epithelial cells.

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“…The oviductal environment can support embryonic growth up to the blastocyst stage across a wide range of species following transspecies transfer. Ligated rabbit oviducts have been used extensively for the development of embryos from many species including sheep [6,43], cattle [9,20,74], and recovery at Day 14, demonstrating that bovine embryos can undergo continued development in the reproductive tract of ewes when transferred either as 4-cell embryos or as expanded or hatched blastocysts.…”

Section: Embryo Culture In Vivo Vs In Vitromentioning

confidence: 99%

Factors influencing oocyte and embryo quality in cattle

et al. 2001

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Comparison between culture of bovine embryos in vitro versus development in rabbit oviducts and in vivo

Cited by 13 publications

References 14 publications

Review: Recent advances in bovine in vitro embryo production: reproductive biotechnology history and methods

Review: Recent advances in bovine in vitro embryo production: reproductive biotechnology history and methods

Factors affecting proliferation and dedifferentiation of primary bovine oviduct epithelial cells in vitro

Factors influencing oocyte and embryo quality in cattle

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