Comparison between Cytokine Concentration in Follicular Fluid of Poor and High Responder Patients and their Influence of ICSI‐outcome

Abstract

There was no significant difference between IGF-I, IL-6, IL-8, EGF and GM-CSF concentrations of low and high responder patients. Besides, PDGF was significantly (P = 0.007) higher in high responder compared with low responder patients. Moreover, in poor responder patients, a negative correlation was found between PDGF and fertilization rate. However, the cytokine levels in FF of the patients undergoing controlled ovarian hyperstimulation for ICSI could not be used as a marker of oocyte fertilization and implantation potential.

The human ovarian follicular fluid level of interleukin-8 is associated with follicular size and patient age

“…No correlation was found by previous investigators (13)(14)(15) between IL-8 concentration and fertilization rates, PRs, or IVF/ICSI outcome. Our results concur with these studies.…”

“…Previous studies have demonstrated the presence of IL-8 within human ovarian follicular fluid (FF) of both IVF patients (6,(11)(12)(13)(14)(15) and normally cycling patients (8,12). Most of these previous studies have measured cytokine levels in pooled FF samples without taking into account that the levels detected may vary based on the size of the follicle.…”

“…Some studies have noted a correlation of IL-8 with follicular size (6,9,11); however, these studies are limited by the inclusion of follicles of only large or mature size and small sample size. In addition, few investigators have sought to correlate IL-8 with IVF cycle characteristics or outcomes (13)(14)(15) and no previous reports investigated a relationship between IL-8 concentration and patient age.…”

Cytokine and nitric oxide concentrations in follicular fluid and blood serum of patients undergoing assisted reproductive treatment: relationship to outcome

“…Conversely, in another study, serum IL-6 levels were found to be correlated negatively with E2 levels (17). FF IL-6 concentrations were also compared between low and high responder patients undergoing ART, and no difference was found (20). In our study, the peak serum E2 levels were similar between the two groups and we did not include the PCOS cases in the study.…”

Does Peri-follicular Blood Flow On The Day Of Ovum Pick-up Predict The Likelihood Of Retrieving An Oocyte?

“…The extent to which the follicular microenvironment affects oocyte competence and as 27,28 . Biochemical analysis of human follicular fluid obtained in stimulated cycles has demonstrated significant differences in concentrations of steroids, growth factors, enzymes and cytokines among follicles at oocyte recovery time 29,30 . This study has some limitations.…”