comparison between DNA synthesis and mitosis in uninvolved and involved psoriatic epidermis and normal epidermis

Goodwin, Peter; Hamilton, Susan; Fry, Lionel

doi:10.1111/j.1365-2133.1973.tb07587.x

Cited by 68 publications

(31 citation statements)

References 11 publications

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“…Although a large number of publications might lead to the conclusion that uninvolved psoriatic skin possesses several features similar to involved psoriatic skin, it is important to note that, in the literature, results about uninvolved skin are still often contradictory. For example, some studies demonstrated that cell proliferation in uninvolved psoriatic skin was similar to what was observed in involved skin (Barker et al ., ; Harper et al ., ), whereas others showed that uninvolved psoriatic cell proliferation rather mimicked normal skin (Goodwin et al ., ; Weinstein and Frost, ). Pershing et al .…”

Section: Introductionmentioning

confidence: 99%

Characterization of a psoriatic skin model produced with involved or uninvolved cells

Jean

Leroy

Duque-Fernandez

et al. 2012

J Tissue Eng Regen Med

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Current knowledge suggests that uninvolved psoriatic skin could demonstrate characteristics associated with both normal and involved psoriatic skins. However, the triggering factor allowing the conversion of uninvolved skin into a psoriatic plaque is not fully understood. To counter this lack of information, we decided to develop pathological skin substitutes produced with uninvolved psoriatic cells, in order to better characterize the uninvolved psoriatic skin. Substitutes were produced using the self-assembly approach. Macroscopic, immunohistochemical, permeability and physicochemical results showed that involved substitutes had a thicker epidermis, higher cell proliferation, abnormal cell differentiation and a more permeable and disorganized stratum corneum compared with normal substitutes. Various results were observed using uninvolved cells, leading to two proposed profiles: profile 1 was suggested for uninvolved skin substitutes mimicking the results obtained with normal skin substitutes; and profile 2 was dedicated to those mimicking involved skin substitutes in all aspects that were analysed. In summary, uninvolved substitutes of profile 1 had a thin, well-organized epidermis with normal cell proliferation and differentiation, such as observed with normal substitutes, while uninvolved substitutes of profile 2 showed an inverse trend, i.e. a thicker epidermis, higher cell proliferation, abnormal cell differentiation and a more disorganized and more permeable stratum corneum, such as seen with involved substitutes. The results suggest that uninvolved substitutes could demonstrate characteristics associated with both normal or involved psoriatic skins.

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Section: Introductionmentioning

confidence: 99%

Characterization of a psoriatic skin model produced with involved or uninvolved cells

Jean

Leroy

Duque-Fernandez

et al. 2012

J Tissue Eng Regen Med

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“…The increased proliferation of epidermal cells is not restricted to the psoriatic lesion but has also been demonstrated in elinieMly unaffected skin (4).…”

Section: Discussionmentioning

confidence: 96%

Rabbit antibodies against the major internal protein of a retrovirus-like particle bind to epidermal cells in psoriatic skin

Iversen¹,

Rødahl²,

Dalen³

1985

Archives of Virology

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A rabbit antiserum against the major internal protein, p27, of a psoriasis associated retrovirus-like particle has been used in indirect fluorescence microscopy of biopsies from psoriatic skin. The analysis indicate expression of p27 antigen in epithelial cells in psoriatic lesions and in clinically uninvolved psoriatic skin. A reaction of the antiserum with dermal vessel walls in the lesion was also observed.

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“…In these studies Ralfs et al (1980) have demonstrated a five-fold higher rate of entry of cells into S than into mitosis. An earlier study of Goodwin, Hamilton & Fry (1973) had shown that the ratio of labelled cells: mitotic cells was 27:1 for psoriatic and 450:1 for normal epidermis. Large labelled cell: mitotic cell ratios have more recently been confirmed in normal epidermis by the more detailed studies of Cample John & Gelfant (1981).…”

mentioning

confidence: 92%

An attempt to use vincristine and colcemid to measure proliferative rates in normal human epidermis in vivo

1981

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A range of doses of vincristine (0.5-10 micrograms) or colcemid (5-100 micrograms) injected intradermally into normal volunteers failed to yield any significant accumulation of arrested mitoses. This was despite an apparently successful block of cells in metaphase by the highest three doses of each drug. Possible reasons for the failure to see accumulation are discussed. The results suggest that normal human epidermis is not a suitable system for application of the metaphase-arrest technique using intradermal injection.

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comparison between DNA synthesis and mitosis in uninvolved and involved psoriatic epidermis and normal epidermis

Cited by 68 publications

References 11 publications

Characterization of a psoriatic skin model produced with involved or uninvolved cells

Characterization of a psoriatic skin model produced with involved or uninvolved cells

Rabbit antibodies against the major internal protein of a retrovirus-like particle bind to epidermal cells in psoriatic skin

An attempt to use vincristine and colcemid to measure proliferative rates in normal human epidermis in vivo

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