Comparison between HPLC and HPTLC densitometry for the determination of icariin from Epimedium koreanum extracts

Abstract

Dry extracts of the aerial parts of Epimedium koreanum were quantified by HPLC and high performance TLC (HPTLC). A gradient HPLC method was used for the quantification of the prenylflavone glycoside icariin at 270 nm. A direct HPTLC assay was developed for the determination of icariin at 270 nm. The UV detection of both analytical assays were used to examine the purity of icariin peaks and compared with the standards. The assays provide good accuracy, reproducibility, and selectivity for the quantitative analysis of icariin. The icariin contents of five different dry extracts were compared by HPLC and HPTLC densitometry. The quantitative results of both analytical methods did not show any statistically significant differences between them, although a trend to slightly lower mean values could be found for the HPLC method.

Separation and evaluation of free radical‐scavenging activity of phenol components of Emblica officinalis extract by using an HPTLC–DPPH• method

“…An HPTLC determination using a modern scanner with diode array detector (DAD) makes it possible to apply the extract containing solution directly on the HPTLC plates without prior preparation steps [14,15].…”

Separation and evaluation of free radical‐scavenging activity of phenol components of green, brown, and black leaves of Bergenia crassifolia by using HPTLC‐DPPH• method

“…A HPTLC determination using a modern scanner with diode array detector (DAD) makes it possible to apply the extract containing solution directly on the HPTLC plates without prior preparation steps [12,13].…”

Comparison Between HPLC and HPTLC Densitometry for the Determination of 11-keto-Beta-boswellic acid and 3- acetyl-11-keto-Beta-boswellic acid from Boswellia serrata Extract.

“…Similar studies comparing HPLC and HPTLC methods for determination of ethanobotanicals have also been reported. 30,31 The major advantage of HPTLC over HPLC is reducing analysis time and cost per analysis. 32 Another advantage of HPTLC discussed in the previously reported study is the capability to detect more compounds than HPLC.…”