Comparison between in vitro embryo production using Y-sorted sperm and timed artificial insemination with non-sorted sperm to produce crossbred calves

Bezerra, Alexandre de Oliveira; Nicacio, Alessandra Corallo; Menezes, G. R. de O.; Gomes, Rodrigo da Costa; Silva, Luiz Otávio Campos da; Rocha-Frigoni, Nathália Alves de Souza; Mingoti, Gisele Zoccal; Leão, Beatriz Caetano da Silva; Silva, Eliane Vianna da Costa e; Nogueira, É.

doi:10.1016/j.anireprosci.2019.06.013

Cited by 10 publications

(14 citation statements)

References 29 publications

(47 reference statements)

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“…In a study on Nelore cows, the pregnancy rate of vitrified embryos was found to be 5% lower than that of fresh embryos. 3 In line with these results, a national survey of bovine ET outcomes in Japan revealed that the pregnancy rate of frozen ET was 5% lower than that of fresh counterparts ( http://www.maff.go.jp/j/chikusan/sinko/lin/l_katiku/attach/pdf/index‐10.pdf ). Therefore, it is important to address that cryopreservation‐induced damage in embryo and oocytes and develop possible countermeasures.…”

Section: Introductionmentioning

confidence: 83%

Resveratrol enhanced mitochondrial recovery from cryopreservation‐induced damages in oocytes and embryos

Iwata

2021

Reprod Medicine & Biology

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Embryo transfer (ET) is a major assisted reproductive technology (ART) used for domestic animals and humans worldwide. In industrialized countries, an increasing number of women get pregnant using ET technology. Success in ET depends on the quality of embryos, which are affected by physiological conditions of oocyte donors such as obesity and diabetes, maternal age, and an ART per se, including in vitro culture and cryopreservation. Cryopreservation is pivotal in ART and supports the widespread use of the embryos collected from genetically and/or commercially valuable domestic animals, as it increases opportunity for pregnancy. Cryopreservation methods have improved over decades; oocytes that are usually difficult to cryopreserve now have high survival rate (over 80%) after

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Section: Introductionmentioning

confidence: 83%

Resveratrol enhanced mitochondrial recovery from cryopreservation‐induced damages in oocytes and embryos

Iwata

2021

Reprod Medicine & Biology

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“…A r t i c l e development and producing offspring [4,6,[10][11][12][13]. However, studies on embryo development and pregnancy rate according to oocyte sources are limited in camels.…”

Section: A C C E P T E Dmentioning

confidence: 99%

“…However, studies on embryo development and pregnancy rate according to oocyte sources are limited in camels. Several studies have reported that the quality of the oocyte cytoplasm is different depending on the source of oocyte collection method [6][7][8]10]. The capacity of embryo development collected with OPU-derived, in vivo-matured oocytes showed increased cleavage and blastocyst formation [6,10,14,15].…”

Section: A C C E P T E Dmentioning

confidence: 99%

“…Various types of factors affect reprogramming, including donor cell type [ 5 ], activation of oocytes [ 6 ], and oocyte cytoplasm [ 6 ]. Among them, the quality of oocytes is an important factor to consider for improving embryo development and producing offspring [ 4 , 6 , 10 – 13 ]. However, studies on embryo development and pregnancy rate according to oocyte sources are limited in camels.…”

Section: Introductionmentioning

confidence: 99%

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Development and pregnancy rates of Camelus dromedarius-cloned embryos derived from in vivo- and in vitro-matured oocytes

Son¹,

Jeong²,

Jeong³

et al. 2022

Anim Biosci

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Objective: The present study evaluated the efficiency of embryo development and clinical pregnancy of somatic cell nuclear transfer (SCNT) embryos using different source-matured oocytes in Camelus dromedarius.Methods: Camelus dromedarius embryos were produced by SCNT using in vivo-and in vitromatured oocytes. In vitro embryo developmental capacity of reconstructed embryos was evaluated. To confirm the efficiency of pregnancy and live birth rates, a total of 72 blastocysts using in vitromatured oocytes transferred into 45 surrogates and 95 blastocysts using in vivo-matured oocytes were transferred into 62 surrogates by transvaginal method. Results:The collected oocytes derived from ovum pick up (OPU) showed higher maturation potential into metaphase II oocytes than oocytes from the slaughterhouse. The competence of cleavage, and blastocyst were also significantly higher in in vivo-matured oocytes than in vitro-matured oocytes.After embryo transfer, 11 pregnant and 10 live births were confirmed in in vivo-matured oocytes group, and 2 pregnant and 1 live birth were confirmed in in vitro-matured oocytes group. Furthermore, blastocysts produced by in vivo-matured oocytes resulted in significantly higher early pregnancy and live birth rates than in vitro-matured oocytes. Conclusion:In this study, SCNT embryos using in vivo-and in vitro-matured camel oocytes were successfully developed, and pregnancy was established in recipient camels. We also confirmed that in vivo-matured oocytes improved the development of embryos and the pregnancy capacity using the blastocyst embryo transfer method.

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“…Afterword, the final concentration (2 X 10 6 spermatozoon ml -1 ) was obtained by adding 250 µl of the previous suspension in TALP medium to each fertilization well. COCs were incubated for 24 h in 5% CO in humidified air at 39 °C (De Oliveira Bezerra et al, 2019; with some modifications). After the incubation period, the fertilized oocytes (zygotes) were investigated by the emergence of the two pronuclei.…”

Section: In Vitro Fertilization (Ivf)mentioning

confidence: 99%

Effects of Various Levels of Luteinizing Hormone and Caprine Follicular Fluid on In Vitro Embryo Production of Shami Goat

2021

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In the current study, the hypothesis of the effects of luteinizing hormone (LH) and follicular fluid (FF) derived from follicles of varying size on in vitro embryo production of the Shami goat breed were tested. The caprine follicular fluid (cFF) was obtained from healthy female’s ovaries by aspiration method and classified into two main classes (follicles with a diameter of ≤ 2mm and ≥3mm). The resulting cFF was added to the culture medium TCM-199 through six Treatments (A, B and C with a source of follicle size of ≤ 2mm; D, E and F with a source of size of ≥3mm). LH was added only to four of the previous Treatments with the levels of 50 µg ml-1 (B and E) and100 µg ml-1 (C and F). Results of the study showed that the oocytes incubated in Treatment F achieved a clear superiority (p=0.001) in the rates of maturation (87.0%), fertilization (80.0%) and cleavage (82.3%). The oocytes incubated in the same Treatment (F) continued to outperform (p= 0.006) by achieving the best rates across cleavage stages at 2-16 cell (16%; the lower value of arrest) and blastocyst (42%). Significant differences (P=0.03) were observed among the rates of Type 1embryos (the highest rate: 45.3%; Treatment F) and Type 3 embryos (the highest rate: 45.1%; Treatment A). No significant differences were observed in the rates of morula and Type 2 embryos. It is advised to add 15% of the cFF derived from follicles with a diameter of ≥3mm and 100 µg of LH ml-1 in the maturation media to obtain higher rates of maturation and cleavage of goat oocytes.

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Comparison between in vitro embryo production using Y-sorted sperm and timed artificial insemination with non-sorted sperm to produce crossbred calves

Cited by 10 publications

References 29 publications

Resveratrol enhanced mitochondrial recovery from cryopreservation‐induced damages in oocytes and embryos

Resveratrol enhanced mitochondrial recovery from cryopreservation‐induced damages in oocytes and embryos

Development and pregnancy rates of Camelus dromedarius-cloned embryos derived from in vivo- and in vitro-matured oocytes

Effects of Various Levels of Luteinizing Hormone and Caprine Follicular Fluid on In Vitro Embryo Production of Shami Goat

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