Comparison between intracytoplasmic sperm injection and intracytoplasmic morphologically selected sperm injection in oligo-astheno-teratozoospermia patients

Kim, Hyung Jun; Yoon, Hye Jin; Jang, JinHyeok; Oh, Hwa Soon; Lee, Yong Jun; Lee, Won Don; Yoon, So Jeong; Lim, Jin Ho

doi:10.5653/cerm.2014.41.1.9

Cited by 24 publications

(17 citation statements)

References 20 publications

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“…It has been shown that IMSI could improve the IVF results in patients with previous ICSI failures. The pregnancy rate was shown to increase in cases with more than two failed ICSI attempts, also in female infertile with unexplained reasons for failure after ICSI with normal spermatozoa (Kim et al, ; Leandri et al, ; Neyer et al, ).…”

Section: Discussionmentioning

confidence: 99%

IMSI procedure improves clinical outcomes and embryo morphokinetics in patients with different aetiologies of male infertility

et al. 2019

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Our objective was to evaluate the effect of IMSI on embryo kinetics and clinical outcomes in patients with different aetiologies of male infertility. A total of 150 couples with different aetiologies of male infertility were randomly divided into ICSI and IMSI treatment groups (n = 75). ICSI was done accordingly. For IMSI group, the sperm selection was done using MSOME criteria and then injected. The zygotes were cultured in time‐lapse monitoring system (TLM) for 3 days. A total of 650 embryos were developed and assessed using TLM in two groups. Data showed the rate of fragmentation had significant correlation with different aetiologies (p = 0.01), and the timing of s1, t4, s2 and t5 occurred significantly later in oligoasthenoteratozoospermia (OAT) patients compared with others (p < 0.05). In IMSI group, there were no differences in the TLM parameters among different aetiologies (p > 0.05). The rates of chemical pregnancy and implantation (37.8% and 38.2% respectively) were insignificantly higher in OAT patients compare to others (p > 0.05). Also, the clinical pregnancy and live birth rates (32% and 32% respectively) were insignificantly higher in teratozoospermia (T) cases. Sperm selection with MSOME parameters and IMSI can improve the embryo morphokinetics and clinical outcomes in couples with male factor infertility, especially for OAT and T patients.

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Section: Discussionmentioning

confidence: 99%

IMSI procedure improves clinical outcomes and embryo morphokinetics in patients with different aetiologies of male infertility

et al. 2019

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“…(2002) developed a method called MSOME ( motile sperm organelle morphology examination ), that selects sperm for ICSI using high magnification (> 6000×) in real time, managing to get a better sperm morphologic evaluation, mainly the analysis of the presence of vacuoles in the head (location, size and numbers), aiding in the selection of sperm with better mitochondrial function, intact chromatin, as well as reducing the number of embryos with aneuploidy (Parmegiani et al, 2012). The technique has been successfully used for oligo-asthenoteratozoospermic patients (Kim et al ., 2014) and for couples that had successive implantation failures, reducing clinic abortion rates by 50% (Oliveira et al, 2011). The downside of this technique is that, apart from the high costs, it may take 60-120 minutes from the selection of the sperm to its injection, depending on semen quality.…”

Section: Discussionmentioning

confidence: 99%

Hyaluronan-binding system for sperm selection enhances pregnancy rates in ICSI cycles associated with male factor infertility

Erberelli¹,

Salgado²,

Pereira³

et al. 2017

JBRA Assisted Reproduction

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Objectives The aim of the present study was to compare two procedures for sperm selection in ICSI cycles - conventional morphology sperm selection (ICSI-PVP) and chemical selection through Hyaluronan-treated petri dishes (PICSI), when male factor was associated.MethodsThe evaluated parameters were semen quality, fertilization and cleavage rates, chemical and clinical pregnancy rates, as well as abortion rate. Fifty-six ICSI cycles were included in this report, 19 cycles using PICSI and 37 using conventional ICSI.ResultsPICSI and ICSI showed, respectively, the following outcome: fertilization rates 71.93% (123/171) and 64.14% (127/198); cleavage rates 95.12% (117/123) and 95.27% (121/127); chemical pregnancy rates 63.15% (12/19) and 27.03% (10/37); clinical pregnancy rates 42.10% (8/19) and 16.21% (6/37); and abortion rates 33.33% (4/12) and 40.00% (4/10). According to both Fisher's Exact Test and Chi-square Test, chemical pregnancy (p = 0.05) and clinical pregnancy (p = 0.09) rates were significantly higher in the PICSI group. p values ≤ 0.05 were consider statistically significant.ConclusionsThe present data indicates that ICSI cycles that used the PICSI technique had a considerably higher chance (≈5 fold) to achieve pregnancy than those who had sperm selected only by morphology assessment. Teratozoospermic patients were those who benefited most with PICSI. Therefore, the technique should be included in laboratory routine with low cost, avoiding the selection of immature sperm with increased rates of peroxidation and DNA fragmentation. Prospective and randomized studies should be applied to strengthen this suggestion.

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“…It has been reported that the negative impact of large nuclear vacuoles (LNV) is perceptible after the onset of the EGA, leading to reduced blastocyst formation [28][29][30][31]. Moreover, LNV also affect ongoing pregnancy rates [31][32][33][34], miscarriage rates [31,33,[35][36][37], and malformation in offspring [38,39].…”

Section: Introductionmentioning

confidence: 99%

The impact of paternal factors on cleavage stage and blastocyst development analyzed by time-lapse imaging—a retrospective observational study

Neyer

Zintz

Stecher

et al. 2015

J Assist Reprod Genet

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Purpose Various time-lapse studies have postulated embryo selection criteria based on early morphokinetic markers. However, late paternal effects are mostly not visible before embryonic genome activation. The primary objective of this retrospective study was to investigate whether those early morphokinetic algorithms investigated by time-lapse imaging are reliable enough to allow for the accurate selection of those embryos that develop into blastocysts, while of course taking into account the correlation with the type of injected spermatozoa. Methods During a period of 18 months, a total of 461 MII oocytes from 43 couples with severe male factor infertility and previous Bexternal^IVF failures after cleavage-stage embryo transfer (ET) were fertilized by intracytoplasmic morphologically selected sperm injection (IMSI). Thereof, 373 embryos were monitored in a time-lapse incubator until ET on day 5. Blastocyst outcome in combination with three previously postulated MKc (cc2: t3-t2, 5-12 h; t3, 35-40 h; t5, 48-56 h) and the morphology of the selected sperm were analyzed. Results A significant increase in the rate of blastocysts (54.0 vs. 36.3 %; P<0.01) and top blastocysts (25.3 vs. 10.8 %; P<0.001) was observed in the group of those meeting all three morphokinetic criteria (MKc3). However, MKc3 were only met in 23.3 % of all embryos. Moreover, TBR was influenced by the type of injected spermatozoa. In both groups, TBR decreased dramatically (MKc3, 35.0 vs. 17.0 %; MKc<3, 14.2 vs. 8.4 %) when class II/III sperm instead of class I were injected. Conclusion Early morphokinetic parameters might give some predictive information but fail to serve as a feasible selective tool for the prediction of blastocyst development given the influence of the type of spermatozoa injected.

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Comparison between intracytoplasmic sperm injection and intracytoplasmic morphologically selected sperm injection in oligo-astheno-teratozoospermia patients

Cited by 24 publications

References 20 publications

IMSI procedure improves clinical outcomes and embryo morphokinetics in patients with different aetiologies of male infertility

IMSI procedure improves clinical outcomes and embryo morphokinetics in patients with different aetiologies of male infertility

Hyaluronan-binding system for sperm selection enhances pregnancy rates in ICSI cycles associated with male factor infertility

The impact of paternal factors on cleavage stage and blastocyst development analyzed by time-lapse imaging—a retrospective observational study

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