Comparison Between Modified Extracellular-Type Trehalose-Containing Kyoto Solution and University of Wisconsin Solution in 18-Hour Pancreas Preservation for Islet Transplantation

Hamada, Eri; Ebi, Nana; Miyagi-Shiohira, Chika; Tamaki, Yoshihito; Nakashima, Yoshiki; Kobayashi, Naoya; Saitoh, Issei; Watanabe, Masami; Kinjo, Takao; Noguchi, Hirofumi

doi:10.1097/mpa.0000000000001104

Cited by 10 publications

(12 citation statements)

References 9 publications

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“…Previous studies have reported that MK solution is superior to UW solution for both 2- and 18-h preservation of pancreatic islets [8,9]. However, we found that IK solution was similarly effective to IU solution for islet purification in this study.…”

Section: Discussioncontrasting

confidence: 71%

“…UW solution has several disadvantages, including inhibition of the activity of collagenases, enzymes used for pancreatic digestion [11,12]. In contrast, MK solution has several advantages, including trypsin inhibition by ulinastatin and lower collagenase inhibition, which does not decrease the energy levels retained in the preserved pancreas [8,9]. However, pancreatic islets are only in contact with each purification solution for 10 to 20 min, which is too short a time for the preservation solution to have any marked effects on the islet cells.…”

Section: Discussionmentioning

confidence: 99%

“…University of Wisconsin (UW) solution is commonly used for pancreas preservation. However, we previously showed that modified extracellular-type trehalose-containing Kyoto (ETK) solution significantly improved the islet yield compared to UW preservation for both 2- and 18-h preservation [8,9]. Modified ETK (MK) solution contains ulinastatin, a trypsin inhibitor, and has a high sodium/low potassium composition.…”

Section: Introductionmentioning

confidence: 99%

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Excellent Islet Yields after 18-h Porcine Pancreas Preservation by Ductal Injection, Pancreas Preservation with MK Solution, Bottle Purification, and Islet Purification Using Iodixanol with UW Solution and Iodixanol with MK Solution

Kuwae

Miyagi-Shiohira

Hamada

et al. 2019

JCM

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Successful islet isolation is the key to successful islet transplantation. Our group recently modified the islet isolation protocol to include pancreatic ductal injection of the preservation solution, pancreas storage in modified extracellular-type trehalose-containing Kyoto (MK) solution, and use of an iodixanol-based purification solution and bottle purification. In this study, we applied these methods to porcine islet isolation after 18-h pancreas preservation and compared two solutions with different compositions in bottle purification. Islet yield before purification was 651,661 ± 157,719 islet equivalents (IE) and 5576 ± 1538 IE/g pancreas weight. An IU solution was made by adding iodixanol to University of Wisconsin solution and an IK solution was made by adding iodixanol to MK solution. The efficacy of the two solutions for islet isolation was compared. There were no significant differences between the two purification methods with regard to islet yield, survival rate, purity, score, or stimulation index. These results indicate that our isolation protocol produces efficient islet yields from prolonged cold-stored pancreas and that IU and IK solutions are equally useful for islet purification.

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Section: Discussioncontrasting

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Excellent Islet Yields after 18-h Porcine Pancreas Preservation by Ductal Injection, Pancreas Preservation with MK Solution, Bottle Purification, and Islet Purification Using Iodixanol with UW Solution and Iodixanol with MK Solution

Kuwae

Miyagi-Shiohira

Hamada

et al. 2019

JCM

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“…Our group previously investigated the clinical application of organ preservation solution[50-56]. We found that the expression level of HGF mRNA did not decrease in ADSCs, even when they were stored in preservation solution for 16 h after separation from adipose tissue.…”

Section: Growth Factors Improving the Symptoms Of Liver Cirrhosismentioning

confidence: 99%

Cytokines in adipose-derived mesenchymal stem cells promote the healing of liver disease

Nahar¹,

Nakashima²,

Miyagi-Shiohira³

et al. 2018

WJSC

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Adipose-derived mesenchymal stem cells (ADSCs) are a treatment cell source for patients with chronic liver injury. ADSCs are characterized by being harvested from the patient’s own subcutaneous adipose tissue, a high cell yield (i.e., reduced immune rejection response), accumulation at a disease nidus, suppression of excessive immune response, production of various growth factors and cytokines, angiogenic effects, anti-apoptotic effects, and control of immune cells via cell-cell interaction. We previously showed that conditioned medium of ADSCs promoted hepatocyte proliferation and improved the liver function in a mouse model of acute liver failure. Furthermore, as found by many other groups, the administration of ADSCs improved liver tissue fibrosis in a mouse model of liver cirrhosis. A comprehensive protein expression analysis by liquid chromatography with tandem mass spectrometry showed that the various cytokines and chemokines produced by ADSCs promote the healing of liver disease. In this review, we examine the ability of expressed protein components of ADSCs to promote healing in cell therapy for liver disease. Previous studies demonstrated that ADSCs are a treatment cell source for patients with chronic liver injury. This review describes the various cytokines and chemokines produced by ADSCs that promote the healing of liver disease.

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“…The replacement of insulin‐secreting beta cells through transplantation of pancreatic islets is a promising therapy for patients with type 1 diabetes 1‐4 . Despite the tremendous progress made in this field, successful islet transplantation is limited by a high loss of islets during pancreas preservation, 5‐9 islet isolation, 10 and transplantation processes 11‐14 . University of Wisconsin (UW) solution is well‐known as the gold standard for organ preservation.…”

Section: Introductionmentioning

confidence: 99%

Pancreas preservation in extracellular‐type p38 inhibitor‐containing solution improves islet yield for porcine islet isolation

Yonaha

Miyagi-Shiohira

Kuwae

et al. 2020

Xenotransplantation

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Background For islet transplantation, pancreas preservation and islet isolation activate p38, which is a member of the stress‐activated group of mitogen‐activated protein kinases (MAPKs). In this study, we evaluated an extracellular‐type p38 inhibitor‐containing (EP) solution with University of Wisconsin (UW) solution, the gold standard for organ preservation. The EP solution has high sodium‐low potassium composition with low viscosity compared to UW solution. Moreover, EP solution contains a recently developed p38 inhibitor (11R‐p38I110) from our laboratory. Methods Porcine pancreata were preserved in UW, EP, or EP‐P solution (EP solution without 11R‐p38I110), and then islet isolation was performed. An optimized number (1500 IE) of isolated islets from each group were transplanted into streptozotocin‐induced diabetic mice. Results The islet yield before and after purification was significantly higher in the EP group than in the UW group. The islet yield before and after purification was not significantly different between the EP and EP‐P groups; however, the EP solution prevented a reduction in the number of islets during culture. Western blot analysis showed that p38 activation was attenuated by EP solution. For islet transplantation into streptozotocin‐induced diabetic mice, pancreas preservation in EP solution improved the outcome of islet transplantation. Conclusions Pancreas preservation with EP solution preserved islet function better than with UW solution. The advantages of EP solution over UW solution may include the inhibition of p38 activity as well as the composition of the solution.

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Comparison Between Modified Extracellular-Type Trehalose-Containing Kyoto Solution and University of Wisconsin Solution in 18-Hour Pancreas Preservation for Islet Transplantation

Cited by 10 publications

References 9 publications

Excellent Islet Yields after 18-h Porcine Pancreas Preservation by Ductal Injection, Pancreas Preservation with MK Solution, Bottle Purification, and Islet Purification Using Iodixanol with UW Solution and Iodixanol with MK Solution

Excellent Islet Yields after 18-h Porcine Pancreas Preservation by Ductal Injection, Pancreas Preservation with MK Solution, Bottle Purification, and Islet Purification Using Iodixanol with UW Solution and Iodixanol with MK Solution

Cytokines in adipose-derived mesenchymal stem cells promote the healing of liver disease

Pancreas preservation in extracellular‐type p38 inhibitor‐containing solution improves islet yield for porcine islet isolation

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