Comparison between Pernasal Flocked Swabs and Nasopharyngeal Aspirates for Detection of Common Respiratory Viruses in Samples from Children

Abu-Diab, Afaf; Azzeh, Maysa; Ghneim, Raed; Ghneim, Riyad; Zoughbi, Madeleine; Turkuman, Sultan; Rishmawi, Nabeel; Issa, Abed-El-Razeq; Siriani, Issa; Dauodi, Rula; Kattan, Randa; Hindiyeh, Musa

doi:10.1128/jcm.00369-08

Cited by 116 publications

(97 citation statements)

References 16 publications

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“…Estudios previos han determinado que la detección viral en hisopados nasofaríngeos es similar a la obtenida en aspirados nasofaríngeos. 11 Dado que aquellos son de mayor accesibilidad, se los ha utilizado en pacientes ambulatorios.…”

Section: Discussionunclassified

Infección respiratoria aguda viral en niños menores de 5 años. Estudio epidemiológico en dos centros de Buenos Aires, Argentina

Vidaurreta

Marcone

Ellis³

et al. 2011

Arch Argent Pediat

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Arch Argent Pediatr 2011;109(4):296-304 / 296 RESUMEN Introducción. Las infecciones respiratorias agudas (IRA) son la mayor causa de hospitalización en edades extremas de la vida. Objetivos. Identificar los agentes etiológicos de IRA viral en niños < 5 años hospitalizados y ambulatorios; describir estacionalidad y caracterís-ticas clínico-epidemiológicas de la enfermedad. Población y métodos. Estudio descriptivo, transversal y multicéntrico en dos centros privados de Buenos Aires, en niños con IRA alta y baja. Se registraron antecedentes, cuadros clínicos y estudios virológicos. Resultados. Se estudiaron 347 pacientes: 235 hospitalizados; 112 ambulatorios. Los hospitalizados fueron menores que los ambulatorios (8 vs. 19 meses, p <0,001), presentaron más frecuentemente bronquiolitis (74% hospitalizados vs. 24% ambulatorios) y neumonía (14% hospitalizados vs. 5% ambulatorios). Solo la edad se asoció significativamente a hospitalización (p= 0,01). Se identificó etiología viral en 81% de los hospitalizados y 57% de los ambulatorios. El virus más frecuente fue rinovirus seguido por virus respiratorio sincicial. Se observó circulación viral durante todo el año, especialmente en otoño e invierno. En pacientes hospitalizados, la mediana de hospitalización fue 3,5 días. Requirieron cuidados intensivos 25 (11%) pacientes, y 7 (3%) recibieron asistencia ventilatoria mecánica. No hubo letalidad. Conclusiones. Las técnicas de diagnóstico virológico permitieron identificar agentes etiológicos en la mayoría de los pacientes hospitalizados y en más de la mitad de los ambulatorios con IRA. La incorporación de RT-PCR para rinovirus, permitió la identificación de este agente etioló-gico. La circulación viral se observó a lo largo de todo el año. La bronquiolitis fue causa de internación en 174/201 (86,5%) niños con IRA y la neumonía en 33/39 (84,6%) niños. Palabras clave: virus respiratorios, infección respiratoria aguda, bronquiolitis, RT-PCR, rinovirus. SUMMARY Introduction. Acute respiratory infections (ARI)are a major cause of hospitalization particularly at the extreme ages of life. Objectives. To determine the viral diagnosis in children < 5 years old with ARI, seasonality, clinical and epidemiological characteristics. Population and methods. A cross-sectional, descriptive and multicenter study was performed. Respiratory samples from patients with ARI hospitalized or outpatients with < 5 days of respiratory symptoms from June 2008 to May 2009 were studied for respiratory viruses. Clinical and demographic data were recorded. Results. A total of 347 patients were enrolled: 234 hospitalized and 112 outpatients. Hospitalized patients were younger compared with outpatients (8 vs. 19 months, p <0.001) and presented more frequently bronchiolitis (74% hospitalized vs. 24% outpatients) and pneumonia (14% hospitalized vs. 5% outpatients). Age was statistically associated with hospitalization (p= 0.01). Viral diagnosis was achieved in 81% hospitalized and 57% of outpatients. Rhinovirus was the most frequent followed by respiratory sync...

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Section: Discussionunclassified

Infección respiratoria aguda viral en niños menores de 5 años. Estudio epidemiológico en dos centros de Buenos Aires, Argentina

Vidaurreta

Marcone

Ellis³

et al. 2011

Arch Argent Pediat

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“…These are considered to be the cornerstones for rapid and accurate diagnosis of the acute respiratory infections caused by this virus. Indeed, the identification of influenza viruses in clinical patient samples is highly dependent on the source of the specimens [18]; samples of nasopharyngeal aspirates and nasopharyngeal washes are superior to other types of samples for the detection of this virus because they yield a large number of epithelial cells during the collection process [19].…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of two rapid influenza tests in comparison to reverse transcription-PCR for influenza A diagnosis

Zazueta-García

Canizalez-Roman

Flores-Villaseñor

et al. 2014

J Infect Dev Ctries

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Introduction: The influenza A virus is responsible for high morbidity and mortality in children and adults worldwide. Thus, a rapid, sensitive, and specific diagnosis tool is required. Methodology: An immunofluorescence assay (DFA) and a lateral-flow immunochromatographic assay were compared with RT-PCR for detection of the influenza A virus in 113 nasopharyngeal wash samples obtained from pediatric patients. Samples were collected between July and December 2009, during the pandemic outbreak of influenza A H1N1/09. Results: The sensitivity, specificity, and positive and negative predictive values obtained for the DFA were 68.97%, 76.63%, 75.47%, and 70%, respectively, while the values obtained for the immunochromatographic assay were 58.62%, 81.82%, 77.27%, and 65.22%, respectively. The frequency of the influenza A virus was 51.33%, and a total of 27 samples were positive for the pandemic influenza A H1N1/09. Conclusions: DFA and the immunochromatographic assay can be important tools for patient care during influenza season and in outbreaks as they usually provide results within 45 minutes. Furthermore, positive results in conjunction with the patient's symptoms could provide a correct diagnosis, thus facilitating appropriate patient management. Nonetheless, the results of these assays still require confirmation by RT-PCR.

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“…Abu-Diab ve ark. (27) çalışmalarında benzer şekil-de DFA ile negatif olan örnekleri GZ-PZR ile pozitif bulmuşlar, flock swab ile alınan örnekle-rin, DFA ile saptanabilecek kadar RSV ile enfekte epitel hücresi içermediğini ancak, daha duyarlı yöntem olan GZ-PZR ile saptanabilecek kadar serbest virüs içerdiğini öne sürmüşlerdir. Bu çalışmada, benzer şekilde GZ-PZR ve hücre kültürü ile saptanan ancak DFA ile saptanamayan beş örneğin, DFA ile saptanabilecek miktarda enfekte epitel hücresi içermediğini, ancak serbest virüs miktarının diğer iki testle saptanabilecek düzeyde olduğunu düşündük.…”

Section: Gereç Ve Yöntemunclassified

“…Örnek alımında esnek flocked eküvyonlar RSV izolasyonunda duyarlı (%89-100) bulunmuş, ayrıca hasta uyumunu da kolaylaştırdığı bildirilmiştir (27)(28)(29) . Bu çalışmada da kullanılan esnek flocked eküv-yonların çocuk hastalardan örneğin alınmasını, taşınmasını ve saklanmasını kolaylaştırdığı görülmüştür.…”

Section: Gereç Ve Yöntemunclassified

Çocuklarda Respiratuvar Sinsityal Virüs (RSV) Enfeksiyonlarının Tanısında Hücre Kültürü ve Direkt Floresan Antikor Testi Yöntemlerinin Karşılaştırılması

Sağlık¹,

Mutlu²,

Öngüt³

et al. 2015

TMCD

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Araştırma ÖZET Amaç: Çocukluk çağında sıklıkla görülen enfeksiyon hastalıklarından biri olan alt solunum yolu enfeksiyonlarının (ASYE) en sık etkeni respiratuvar sinsityal virüs (RSV)'tür. Bu çalışmanın amacı ASYE tanısı alan 0-5 yaş grubundaki çocuklarda RSV'nin hücre kültürü ve direkt floresan antikor (DFA) yöntemleri ile araştırılması ve sonuçların hastaların klinik bulguları ile birlikte değerlendirilmesidir. Gereç ve Yöntem: ASYE tanısı alan 0-5 yaş arasındaki 107 ayaktan (poli-klinik Objective: Respiratory syncytial virus (RSV) is the most common cause of lower respiratory tract infections (LRTI) in children. This study was aimed to investigate the presence of RSV by cell culture and direct fluorescent antibody (DFA) methods in children with LRTI and to evaluate the results in association with the clinical findings of the patients. Materials and Methods: The study included 162 children with LRTI. The age group of the cases was 0-5 years old, of which 107 were outpatients and 55 were inpatients. Nasopharyngeal swab samples were obtained by flocked swabs and transported to the laboratory within the viral transport medium (Copan Diagnostics, Brescia, Italy). Samples were inoculated into the shell-vial cell culture prepared with A549 cells and presence of RSV were investigated in the fixed cells by fluorescence-labeled monoclonal antibodies (Anti-RSV

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Comparison between Pernasal Flocked Swabs and Nasopharyngeal Aspirates for Detection of Common Respiratory Viruses in Samples from Children

Cited by 116 publications

References 16 publications

Infección respiratoria aguda viral en niños menores de 5 años. Estudio epidemiológico en dos centros de Buenos Aires, Argentina

Infección respiratoria aguda viral en niños menores de 5 años. Estudio epidemiológico en dos centros de Buenos Aires, Argentina

Effectiveness of two rapid influenza tests in comparison to reverse transcription-PCR for influenza A diagnosis

Çocuklarda Respiratuvar Sinsityal Virüs (RSV) Enfeksiyonlarının Tanısında Hücre Kültürü ve Direkt Floresan Antikor Testi Yöntemlerinin Karşılaştırılması

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