Comparison between Poly(lactic-co-glycolic acid) Films Contained Natural Polymers on Adhesion and Proliferation of Schwann Cells

Abstract

This study was designed to find an appropriate biomaterial to proliferate Schwann cell (SC). Poly(lactic-coglycolic acid) (PLGA) films mixed with demineralized bone particle (DBP), small intestine submucosa (SIS), and silk were fabricated by a solvent casting method. Analysis of MTT, SEM and RT-PCR were performed to confirm adhesion and proliferation of SC. Contact angle of films was assayed for hydrophilicity of films. We confirmed that PLGA/DBP 20% film showed higher hydrophilicity, promoted adhesion and proliferation of SC than other films. It was concluded that PLGA/DBP film can be applied for the scaffold biomaterials for the regeneration of central nerve system. Keywords: schwann cell, demineralized bone particle (DBP), small intestine submucosa (SIS), silk, PLGA hybridized film. 서 론