Comparison between soluble components of adenovirus types 3 and 16 and of the intermediate strain 3–16 (the San Carlos Agent)

Norrby, Erling; Skaaret, P.

doi:10.1016/0042-6822(68)90137-2

Cited by 34 publications

(25 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This and the fact that Ad9 and Adl5/H9 are related in HI tests correspond well to results on physical properties of com plete and incomplete hemagglutinins [Norrby, 1968], which were identical for Ad9 and Adl5/H9 but different for Adl5. It is likely that Adl5/H9 shares the species-specific fiber component y with Ad9, and the e hexon determinant with Adl5 and Ad29.…”

Section: Discussionmentioning

confidence: 58%

Characterization of Adenovirus 15/H9 Intermediate Strains

et al. 1985

View full text Add to dashboard Cite

17 adenovirus strains from various parts of the United States and Europe were characterized by immunological and biochemical methods. They were antigenically related to adenovirus 15 (Ad15) in neutralization tests. In hemagglutination-inhibition tests, a relationship was seen to Ad9, but not to Ad15. These strains were named Ad15/H9 intermediate strains. The relatedness of prototypes Ad15 and Ad29 in neutralization tests and of Ad9 and Ad8 in hemagglutination-inhibition tests was also found in the strains studied. The DNAs of these strains were analyzed by use of restriction endonucleases. After cleavage with the enzymes BamHl, Bglll, BstElI, Hindlll, and Smal, fragment patterns of Ad15/H9 strains were found to be different from the prototypes and were more closely related to Ad9 than to Ad15. Furthermore, the 17 strains could be classified into six different genome types, a-f.

show abstract

Section: Discussionmentioning

confidence: 58%

Characterization of Adenovirus 15/H9 Intermediate Strains

et al. 1985

View full text Add to dashboard Cite

show abstract

“…Unfortunately, we could not obtain HAdV-29 antiserum. HAdV-15 and -29 showed cross-reactions in the SN test (8,23,24,36), and HAdV-29 was suggested to be a recombinant variant of HAdV-15 (20). Among the 2307-S strain, HAdV-15, and HAdV-29, loops 1 and 2 in the hexon gene, which are SN targets, are identical ( Fig.…”

Section: Discussionmentioning

confidence: 95%

“…The intertypic strain between HAdV-9 and -15 was detected by both SN and HI tests in Europe in 1968 and was reported as HAdV-15/H9 (23). HAdV-15/H9 was neutralized by both HAdV-15 and -29 antisera and showed weak cross-reactivity against HAdV-9 antiserum (23,36).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Complete Genome Analysis of a Novel Intertypic Recombinant Human Adenovirus Causing Epidemic Keratoconjunctivitis in Japan

et al. 2011

View full text Add to dashboard Cite

For 4 months from September 2008, 102 conjunctival swab specimens were collected for surveillance purposes from patients across Japan suspected of having epidemic keratoconjunctivitis (EKC). Human adenovirus (HAdV) DNA was detected in 61 samples by PCR, though the HAdV type for 6 of the PCR-positive samples could not be determined by phylogenetic analysis using a partial hexon gene sequence. Moreover, for 2 months from January 2009, HAdV strains with identical sequences were isolated from five conjunctival swab samples obtained from EKC patients in five different regions of Japan. For the analyses of the 11 samples mentioned above, we determined the nucleotide sequences of the entire penton base, hexon, and fiber genes and early 3 (E3) region, which are variable regions among HAdV types, and compared them to those of other HAdV species D strains. The nucleotide sequences of loops 1 and 2 in the hexons of all 11 samples showed high degrees of identity with those of the HAdV type 15 (HAdV-15) and HAdV-29 prototype strains. However, the fiber gene and E3 region sequences showed high degrees of identity with those of HAdV-9, and the penton base gene sequence showed a high degree of identity with the penton base gene sequences of HAdV-9 and -26. Moreover, the complete genome sequence of the 2307-S strain, which was isolated by viral culture from 1 of the 11 samples, was determined. The 2307-S strain was a recombinant HAdV between HAdV-9, -15, -26, -29, and/or another HAdV type; however, the recombination sites in the genome were not obvious. We propose that this virus is a novel intertypic recombinant, HAdV-15/29/H9, and may be an etiological agent of EKC.

show abstract

“…Type-specific antigenic determinants of hexon have been demonstrated on the surface of the virion and a common group-specific moiety appears to be buried internally (Haase & Pereira, 1972;Kjell6n & Pereira, 1968;Mautner & Willcox, 1974;Norrby, 1969a;Willcox & Mautner, 1976a, b). The antigenic properties of Ads are further complicated by naturally 0001-0755 © 1992 SGM occurring recombinants between closely related serotypes, which may possess hexon specificities of one type and fibre specificities of another (Norrby, 1968).…”

Section: Introductionmentioning

confidence: 99%

Antipeptide antisera define neutralizing epitopes on the adenovirus hexon

Toogood

Crompton

Hay

1992

Journal of General Virology

106

View full text Add to dashboard Cite

The adenovirus (Ad) hexon contains both group-and type-specific antigenic determinants. To identify the latter, peptides were synthesized corresponding to residues 281 to 292 from loop 1 and 441 to 455 from loop 2 of the Ad2 hexon. These sequences display typespecific variation and have been shown by X-ray crystallography to be present on the surface of the virion. Antisera raised against the peptides bound both peptide and the native hexon in ELISA, and blocked virus infectivity, as determined by immunofluorescence or neutralization assays. The loop 1 peptide was shown to inhibit binding of the corresponding antiserum to the native hexon in ELISA and to abolish its neutralizing activity. Neither the loop 1-nor loop 2-specific antiserum neutralized the infectivity of Ad4 or Ad40. Neutralization did not appear to result from aggregation of virus particles and thus their inability to attach to the cell, because virions treated with immune serum were internalized to the same extent as those treated with preimmune serum. Examination of the immune response elicited by Ad2 infection revealed that antibodies directed against the L 1 and L2 epitopes were also present in human serum. Thus, the variable regions exposed on the surface of the Ad2 hexon represent type-specific neutralizing antigenic determinants.

show abstract

Comparison between soluble components of adenovirus types 3 and 16 and of the intermediate strain 3–16 (the San Carlos Agent)

Cited by 34 publications

References 14 publications

Characterization of Adenovirus 15/H9 Intermediate Strains

Characterization of Adenovirus 15/H9 Intermediate Strains

Complete Genome Analysis of a Novel Intertypic Recombinant Human Adenovirus Causing Epidemic Keratoconjunctivitis in Japan

Antipeptide antisera define neutralizing epitopes on the adenovirus hexon

Contact Info

Product

Resources

About