Comparison between the effects of fusicoccin, Tunicamycin, and Brefeldin A on programmed cell death of cultured sycamore (Acer pseudoplatanus L.) cells

Malerba, Massimo; Crosti, Paolo

doi:10.1007/s00709-004-0053-7

Cited by 29 publications

(13 citation statements)

References 63 publications

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“…BiP has also been demonstrated to regulate pro-apoptotic pathways that emanate from the ER when ER homeostasis cannot be restored ( Reddy et al , 2003 ; Malhotra and Kaufman, 2007 ). Prolonged ER stress (accumulation of unfolded or misfolded proteins) has been shown to induce cell death, apoptotic-like responses and leaf senescence in plants ( Crosti et al , 2001 ; Malerba et al , 2004 ; Zuppini et al , 2004 , Wang et al , 2007 ; Costa et al , 2008 ; Watanabe and Lam, 2008 ). The effect of UPR on leaf senescence has recently been associated with multimeric G-protein signalling ( Wang et al , 2007 ).…”

Section: Discussionmentioning

confidence: 99%

The ER luminal binding protein (BiP) mediates an increase in drought tolerance in soybean and delays drought-induced leaf senescence in soybean and tobacco

Valente

Faria

Soares-Ramos

et al. 2008

Journal of Experimental Botany

166

170

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The ER-resident molecular chaperone BiP (binding protein) was overexpressed in soybean. When plants growing in soil were exposed to drought (by reducing or completely withholding watering) the wild-type lines showed a large decrease in leaf water potential and leaf wilting, but the leaves in the transgenic lines did not wilt and exhibited only a small decrease in water potential. During exposure to drought the stomata of the transgenic lines did not close as much as in the wild type, and the rates of photosynthesis and transpiration became less inhibited than in the wild type. These parameters of drought resistance in the BiP overexpressing lines were not associated with a higher level of the osmolytes proline, sucrose, and glucose. It was also not associated with the typical drought-induced increase in root dry weight. Rather, at the end of the drought period, the BiP overexpressing lines had a lower level of the osmolytes and root weight than the wild type. The mRNA abundance of several typical drought-induced genes [NAC2, a seed maturation protein (SMP), a glutathione-S-transferase (GST), antiquitin, and protein disulphide isomerase 3 (PDI-3)] increased in the drought-stressed wild-type plants. Compared with the wild type, the increase in mRNA abundance of these genes was less (in some genes much less) in the BiP overexpressing lines that were exposed to drought. The effect of drought on leaf senescence was investigated in soybean and tobacco. It had previously been reported that tobacco BiP overexpression or repression reduced or accentuated the effects of drought. BiP overexpressing tobacco and soybean showed delayed leaf senescence during drought. BiP antisense tobacco plants, conversely, showed advanced leaf senescence. It is concluded that BiP overexpression confers resistance to drought, through an as yet unknown mechanism that is related to ER functioning. The delay in leaf senescence by BiP overexpression might relate to the absence of the response to drought.

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Section: Discussionmentioning

confidence: 99%

The ER luminal binding protein (BiP) mediates an increase in drought tolerance in soybean and delays drought-induced leaf senescence in soybean and tobacco

Valente

Faria

Soares-Ramos

et al. 2008

Journal of Experimental Botany

166

170

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“…TMV‐p3‐inoculated N. benthamiana leaves were infiltrated with 20 μ m FC or 10 μg/mL TU (Koizumi et al ., ; Liu et al ., ; Malerba et al ., , ) prepared in Murashige and Skoog (MS) medium. Three 1.2‐cm‐diameter leaf discs were taken at 5 dpi (Il‐Pyung, ), incubated in 0.25% (w/v in double‐distilled H 2 O) Evans blue stain for 30 min and rinsed with double‐distilled H 2 O. Stain was extracted using 3 mL of a solution of 50% (v/v) methanol and 1% (w/v) sodium dodecylsulphate (SDS) for 60 min at 50 °C.…”

Section: Methodsmentioning

confidence: 99%

TGBp3 triggers the unfolded protein response and SKP1‐dependent programmed cell death

Chen

Payton

et al. 2013

Molecular Plant Pathology

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The Potato virus X (PVX) triple gene block protein 3 (TGBp3), an 8-kDa membrane binding protein, aids virus movement and induces the unfolded protein response (UPR) during PVX infection. TGBp3 was expressed from the Tobacco mosaic virus (TMV) genome (TMV-p3), and we noted the up-regulation of SKP1 and several endoplasmic reticulum (ER)-resident chaperones, including the ER luminal binding protein (BiP), protein disulphide isomerase (PDI), calreticulin (CRT) and calmodulin (CAM). Local lesions were seen on leaves inoculated with TMV-p3, but not TMV or PVX. Such lesions were the result of TGBp3-elicited programmed cell death (PCD), as shown by an increase in reactive oxygen species, DNA fragmentation and induction of SKP1 expression. UPR-related gene expression occurred within 8 h of TMV-p3 inoculation and declined before the onset of PCD. TGBp3-mediated cell death was suppressed in plants that overexpressed BiP, indicating that UPR induction by TGBp3 is a pro-survival mechanism. Anti-apoptotic genes Bcl-xl, CED-9 and Op-IAP were expressed in transgenic plants and suppressed N gene-mediated resistance to TMV, but failed to alleviate TGBp3-induced PCD. However, TGBp3-mediated cell death was reduced in SKP1-silenced Nicotiana benthamiana plants. The combined data suggest that TGBp3 triggers the UPR and elicits PCD in plants.

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“…Arabidopsis seedlings (Crosti, Malerba & Bianchetti 2001;Malerba, Cerana & Crosti 2004;Zuppini, Navazio & Mariani 2004;Iwata & Koizumi 2005b;Watanabe & Lam 2008). Further characterization of the death phenomenon brought to light cytological and molecular events related to PCD; these included accumulation of hydrogen peroxide, cytochrome c leakage, enhanced HSR203J gene expression, activation of caspase-like enzymes, cytoplasmic shrinkage and chromatin condensation.…”

Section: Endomembrane System and Programmed Cell Death 1455mentioning

confidence: 99%

“…Endomembrane system and programmed cell death 1459 sycamore cell culture, the use of brefeldin A, an antibiotic known to induce apoptosis by disrupting ER-to-GA protein trafficking, was found to trigger a cell death process accompanied by several PCD hallmarks (Crosti et al 2001;Malerba et al 2004). Yet, it is unclear if treatment with brefeldin A mimics specific conditions that cause the GA to induce PCD or if it triggers an ER-originating UPRdependent cell death pathway(s) because of GA collapse.…”

Section: Ga and Secretory Pathway In Pcd: Insights From Plantsmentioning

confidence: 99%

“…Yet, it is unclear if treatment with brefeldin A mimics specific conditions that cause the GA to induce PCD or if it triggers an ER-originating UPRdependent cell death pathway(s) because of GA collapse. The fact that the ER stressor,TM, when applied to sycamore cell culture induces a similar set of physiological events as brefeldin A (Malerba et al 2004) could argue for the second hypothesis. The recent identification of a new GA-resident apoptosis regulator (Gubser et al 2007) that has homologs in all sequenced plant genomes (Cacas, unpublished data) would, however, sustain the first scenario.…”

Section: Ga and Secretory Pathway In Pcd: Insights From Plantsmentioning

confidence: 99%

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Devil inside: does plant programmed cell death involve the endomembrane system?

Cacas

2010

Plant, Cell &Amp; Environment

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Comparison between the effects of fusicoccin, Tunicamycin, and Brefeldin A on programmed cell death of cultured sycamore (Acer pseudoplatanus L.) cells

Cited by 29 publications

References 63 publications

The ER luminal binding protein (BiP) mediates an increase in drought tolerance in soybean and delays drought-induced leaf senescence in soybean and tobacco

The ER luminal binding protein (BiP) mediates an increase in drought tolerance in soybean and delays drought-induced leaf senescence in soybean and tobacco

TGBp3 triggers the unfolded protein response and SKP1‐dependent programmed cell death

Devil inside: does plant programmed cell death involve the endomembrane system?

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