Comparison of agar gel immunodiffusion test, rapid slide agglutination test, microbiological culture and PCR for the diagnosis of canine brucellosis

Keid, Lara Borges; Soares, Rodrigo Martins; Vasconcellos, Sílvio Arruda; Megid, Jane; Salgado, Vanessa Riesz; Richtzenhain, Leonardo José

doi:10.1016/j.rvsc.2008.05.012

Cited by 74 publications

(89 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nonspecific reactions are known to occur when lipopolysaccharide antigens are used with the AGID technique; however, the line created by the precipitated serum is different from that observed in sera diagnosed as false-positive (GREENE; CARMICHAEL, 2006 (VARGAS et al, 1966). These differences in infections levels can be associated with the laboratory technique used, the type of animal (household, stray, or shelter dogs) evaluated When serology (AGID, rapid agglutination test with and without 2-mercaptoethanol), microbiological culture, and polymerase chain reaction (PCR) were compared for the efficient diagnosis of B. canis (KEID et al, 2009), it was demonstrated that a significant proportion of serological evaluations yielded false-negative results. These authors also indicated the importance of direct evaluations (PCR and/or bacteriological culture) to improve the efficiency of the diagnosis of CB; the absence and/or non-utilization of these might techniques be partially responsible for the reduced prevalence indices described in this and other studies.…”

mentioning

confidence: 99%

Serological detection of Brucella canis in shelter dogs from Northern Paraná

et al. 2012

View full text Add to dashboard Cite

This study evaluated the presence of circulating antibodies of anti-Brucella canis and anti-B. abortus in shelter dogs maintained in four cities of Northern Paraná. Serum samples of 100 dogs, of both sexes, from the cities of Apucarana, Arapongas, Londrina and Rolândia were collected for the determination of the serological presence of B. canis by the utilization of agarose gel immunodiffusion and B. abortus by the buffered acidified antigen assay. Only 4% (4/100) of the samples evaluated demonstrated seroreactivity to B. canis, with seropositivity varying between 4.17 -10%. However, positive samples originated only from the cities of Apucarana (4.17%; 2/48) and Londrina (10%; 2/20). Seroreactivity to B. abortus antibodies was not verified within the canine population evaluated. These results suggest that B. canis is circulating within the dog population of Northern Paraná. Key words: Canine disease, epidemiology, brucellosis, serology ResumoEste estudo avaliou a presença de anticorpos circulantes anti-Brucella canis e anti-B. abortus em cães mantidos em abrigos situados em quatro municípios da região Norte do Paraná. Amostras séricas de 100 cães, de ambos os sexos, oriundos das cidades de Apucarana, Arapongas, Londrina e Rolândia foram colhidas para determinação sorológica da presença de anticorpos contra B. canis por meio da técnica de imunodifusão em gel de agarose e de anticorpos contra B. abortus por meio da prova do antígeno acidificado tamponado. Somente 4% (4/100) das amostras avaliadas foram sororreagentes a B. canis, com soropositividade variando entre 4.17 -10%. Contudo, as amostras positivas foram oriundas somente das cidades de Apucarana (4.17%; 2/48) e Londrina (10%; 2/20). Não foram verificadas amostras sororreagentes a B. abortus na população canina avaliada. Esses resultados sugerem que B. Canis circula na população de cães da região Norte do Paraná.

show abstract

mentioning

confidence: 99%

Serological detection of Brucella canis in shelter dogs from Northern Paraná

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Con el fin de seguir de cerca el curso de la enfermedad en caninos, es pertinente sugerir un estudio de tipo longitudinal, con muestreos periódicos a los caninos de núcleos con antecedentes reproductivos o con seropositividad humana, como el caso encontrado en la región Valle de Aburrá; con el fin de descartar fallas en la detección de IgG en caninos en fases iniciales de la enfermedad, dado que PARP-2ME no detecta IgM, y estos caninos formarán parte de los falsos negativos arrojados por la prueba 6,46,47 . El estudio longitudinal sería de gran utilidad, además, por la condición de ondulante o insidiosa que poseen en general los diferentes tipos de brucelosis 6,35,40,47,48 ; es bastante conocido que tanto las bacteremias, como la circulación de anticuerpos contra Brucella sp., ocurren de forma intermitente, existiendo la posibilidad de coincidir los periodos de abacteremia y seronegatividad con el momento del muestreo en estudios tipo transversal como este, tanto en animales como en personas relacionadas.…”

Section: Discussionunclassified

Factores asociados con la seropositividad a Brucella canis en criaderos caninos de dos regiones de Antioquia, Colombia

et al. 2013

View full text Add to dashboard Cite

El objetivo fue determinar la seroprevalencia a Brucella canis en perros y humanos convivientes en criaderos caninos y explorar los factores de riesgo asociados a la seropositividad. Se tomaron 20 criaderos, en los cuales se realizó diagnóstico serológico por PARP-2ME de 428 caninos y 91 humanos. Se aplicó una encuesta para determinar los factores de riesgo y se analizaron los datos mediante regresión logística. Se determinó una seroprevalencia de 15% en caninos y 9% en humanos convivientes. Se determinaron como factores asociados a la seropositividad canina el historial de seropositividad canina, conservar los caninos seropositivos, historial de aborto, higiene y protección del operario deficientes durante el servicio reproductivo, y procedimiento inseguro durante la atención de abortos. Como factores protectores se establecieron la ubicación rural de los criaderos, facilidad de aseo de los caniles, PARP-2ME premonta, y procedimiento seguro durante la atención de partos. En humanos se determinaron factores asociados: criaderos ubicados en el Valle Aburrá y de tipo urbano.

show abstract

“…While control measures for brucellosis on other animal species include vaccination, at present, there is no available vaccine against B. canis. On the other hand, despite the continuous development of different serological techniques, diagnosis remains a complex issue that is not always reliable (7). Moreover, any ideal canine brucellosis control program should rely on a vaccine that contains protective antigens that do not cause misinterpretation of serological results between infected and vaccinated animals.…”

mentioning

confidence: 99%