Comparison of analytical techniques to quantify malondialdehyde in milk powders

Fenaille, François; Mottier, Pascal; Turesky, Robert J.; Ali, Santo; Guy, Philippe A.

doi:10.1016/s0021-9673(01)00883-4

Cited by 99 publications

(72 citation statements)

References 21 publications

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“…DNP-carbonyl derivatives have absorption maximum in the wavelength range of 350-380 nm, but the DNP-MDA derivative shows the maximum at 307 nm (Fenaille et al 2001). In our previous method using a single wavelength detector, we set the detection wavelength at 340 nm to detect MDA and other carbonyls at the same time, with a sacrifice of the sensitivity (Matsui et al 2009).…”

Section: Quantitation Of Carbonyls On the Ultraviolet Absorbancementioning

confidence: 99%

Acrolein is formed from trienoic fatty acids in chloroplast: A targeted metabolomics approach

Mano

Khorobrykh

Matsui

et al. 2014

Plant Biotechnology

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Aldehydes and ketones produced from lipid peroxides (oxylipin carbonyls) exhibit a variety of biological activities ranging from the induction of defense genes to the irreversible damage to cells. Short oxylipin carbonyls such as acrolein and (E)-2-pentenal are responsible for tissue injury under environmental stress, but their production mechanism remains unclear. In this study, we elucidated the source fatty acids of short oxylipin carbonyls in leaves. We first established a comprehensive analysis of oxylipin carbonyls for quantitation and structural estimation. Carbonyls were extracted from rosette leaves of Arabidopsis thaliana, derivatized with 2,4-dinitrophenylhydrazine and separated with a reverse-phase HPLC equipped with a photodiode array detector and an Fourier transform ion cyclotron resonance mass spectrometer. Thirty-three distinct carbonyls were detected, in which 19 species were identified on the MS/MS spectrum. Using this analysis system, we compared the carbonyl composition in the leaves between two A. thaliana lines that have different fatty acid composition. The mutant fad7fad8, which lacks the biosynthesis of trienoic fatty acids in the plastid, contained significantly lower amounts of malondialdehyde, acrolein and (E)-2-pentenal and higher amounts of acetone, 3-pentanone, and n-hexanal than the wild type Col-0. This difference in the carbonyl composition agreed with the oxidative degradation of dienoic and trienoic fatty acids in vitro, showing that similar non-enzymatic reactions occur in the thylakoid membrane. Consideration of the formation mechanism of acrolein from trienoic fatty acid suggests that membrane lipids in chloroplasts are constitutively oxidized by singlet oxygen.

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Section: Quantitation Of Carbonyls On the Ultraviolet Absorbancementioning

confidence: 99%

Acrolein is formed from trienoic fatty acids in chloroplast: A targeted metabolomics approach

Mano

Khorobrykh

Matsui

et al. 2014

Plant Biotechnology

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“…Malondialdehyde (MDA) derived from polyunsaturated fatty acids with 3 or more double bonds was prepared as described by Fenaille and colleagues (24).…”

Section: Fecal Assays In Rat and Micementioning

confidence: 99%

A Central Role for Heme Iron in Colon Carcinogenesis Associated with Red Meat Intake

et al. 2015

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Epidemiology shows that red and processed meat intake is associated with an increased risk of colorectal cancer. Heme iron, heterocyclic amines, and endogenous N-nitroso compounds (NOC) are proposed to explain this effect, but their relative contribution is unknown. Our study aimed at determining, at nutritional doses, which is the main factor involved and proposing a mechanism of cancer promotion by red meat. The relative part of heme iron (1% in diet), heterocyclic amines (PhIP þ MeIQx, 50 þ 25 mg/kg in diet), and NOC (induced by NaNO 2 þ NaNO 3 ; 0.17 þ 0.23 g/L of drinking water) was determined by a factorial design and preneoplastic endpoints in chemically induced rats and validated on tumors in Min mice. The molecular mechanisms (genotoxicity, cytotoxicity) were analyzed in vitro in normal and Apc-deficient cell lines and confirmed on colon mucosa. Heme iron increased the number of preneoplastic lesions, but dietary heterocyclic amines and NOC had no effect on carcinogenesis in rats. Dietary hemoglobin increased tumor load in Min mice (control diet: 67 AE 39 mm 2 ; 2.5% hemoglobin diet: 114 AE 47 mm 2 , P ¼ 0.004). In vitro, fecal water from rats given hemoglobin was rich in aldehydes and was cytotoxic to normal cells, but not to premalignant cells. The aldehydes 4-hydroxynonenal and 4-hydroxyhexenal were more toxic to normal versus mutated cells and were only genotoxic to normal cells. Genotoxicity was also observed in colon mucosa of mice given hemoglobin. These results highlight the role of heme iron in the promotion of colon cancer by red meat and suggest that heme iron could initiate carcinogenesis through lipid peroxidation. Cancer Res; 75(5); 870-9. Ó2015 AACR.

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“…25 Briefly, MDA standards were prepared by the acid hydrolysis of 1,1,3,3-tetraethoxypropane (Sigma Inc, Sacramento, CA, USA) at 40…”

Section: Lipid Oxidation (Plasma Malondialdehyde)mentioning

confidence: 99%

Boysenberry and blackcurrant drinks increased the plasma antioxidant capacity in an elderly population but had little effect on other markers of oxidative stress

et al. 2007

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BACKGROUND: The consumption of fruit and vegetables promotes good health by protecting against various degenerative diseases. Even though the constituents responsible are not known, some evidence indicates that the antioxidant properties of fruit and vegetable phytochemicals are responsible. Previous studies have shown that blackcurrant and Boysenberry reduce oxidative stress using in vitro cell systems. The aim of this study was to determine if blackcurrant or Boysenberry drinks could improve measures of oxidative stress and inflammation in an elderly population with below-average memory abilities. The intervention parallel study was fully blinded with a placebo control.

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Comparison of analytical techniques to quantify malondialdehyde in milk powders

Cited by 99 publications

References 21 publications

Acrolein is formed from trienoic fatty acids in chloroplast: A targeted metabolomics approach

Acrolein is formed from trienoic fatty acids in chloroplast: A targeted metabolomics approach

A Central Role for Heme Iron in Colon Carcinogenesis Associated with Red Meat Intake

Boysenberry and blackcurrant drinks increased the plasma antioxidant capacity in an elderly population but had little effect on other markers of oxidative stress

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