Comparison of Apoptotic Inducing Effect of Zerumbone and Zerumbone‐Loaded Nanostructured Lipid Carrier on Human Mammary Adenocarcinoma MDA‐MB‐231 Cell Line

Hosseinpour, Mahnaz; Abdul, Ahmad Bustamam; Rahman, Heshu Sulaiman; Abdullah, Rasedee; Yeap, Swee Keong; Ahmadi, Negin; Othman, Hemn Hassan; Chartrand, Max Stanley

doi:10.1155/2014/742738

Cited by 12 publications

(14 citation statements)

References 22 publications

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“…Two plates were assigned to each incubation time of 0, 24, 48, and 72 h. The cells were treated with either 6.5, 12.5, 25, 50, or 100 μ M of doxorubicin (positive control), ZER, NLC, or ZER-NLC [ 23 ]. Negative control wells received 100 μ L RPMI-1640 growth medium only.…”

Section: Methodsmentioning

confidence: 99%

“…ZER-loaded NLC (ZER-NLC) has apoptogenic effect on acute human lymphoblastic leukemia (Jurkat) cell line [ 10 ], murine myelocytic leukemia cell line (WEHI-3B) [ 22 ], human mammary adenocarcinoma (MDA-MB-231) cell line [ 23 ], and murine breast cancer (4T1) cell line [ 24 ]. The anticancer effect of ZER-NLC on canine mammary gland tumor (CMT) is not known.…”

Section: Introductionmentioning

confidence: 99%

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Zerumbone-Loaded Nanostructured Lipid Carrier Induces Apoptosis of Canine Mammary Adenocarcinoma Cells

Foong

Selvarajah

Abdullah

et al. 2018

BioMed Research International

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Canine mammary gland tumor (CMT) is the most common tumor in intact female dog. Zerumbone (ZER) has promising anticancer properties, but plagued with poor water solubility, poor absorption, bioavailability, and delivery to target tissues. To solubilize, ZER was loaded into nanostructured lipid carrier (NLC) to produce ZER-loaded NLC (ZER-NLC). The objectives of this study were to determine the antiproliferative effect and the mode of cell death induced by ZER-NLC and ZER on a canine mammary gland tumor (CMT) adenocarcinoma primary cell line. There was no significant difference (p>0.05) between ZER-NLC and ZER treatments in the inhibition of CMT cell proliferation; thus, the loading of ZER into NLC did not compromise the cytotoxic effect of ZER. Microscopically, ZER-NLC- and ZER-treated CMT cells showed apoptotic cell morphology. ZER-NLC and ZER treatments significantly downregulated the antiapoptotic Bcl-2 and upregulated the proapoptotic Bax gene expressions in CMT cells. Both ZER-NLC and ZER-treated CMT cells showed significant (p<0.0001) increases in caspase-8, -9, and -3/7 protein activities. In conclusion, ZER-NLC induced CMT cell death via regulation of Bcl-2 and Bax gene expressions and caspase activations, indicating the involvement of both the intrinsic and extrinsic pathways of apoptosis. This study provided evidences for the potential of ZER-NLC as an anticanine mammary gland adenocarcinoma chemotherapy.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Zerumbone-Loaded Nanostructured Lipid Carrier Induces Apoptosis of Canine Mammary Adenocarcinoma Cells

Foong

Selvarajah

Abdullah

et al. 2018

BioMed Research International

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“…The cell pellets were washed twice with 1 mL PBS and stained with PBS staining buffer that contained 0.1% triton X-100, 10 mM ethylenediaminetetraacetic acid, 50 μg/mL RNAase A, and 3 μg/mL PI and incubated on ice in dark for 30 minutes. 18 Flow cytometric analysis was conducted using the BD FACS Calibur flow cytometer (BD), and data analysis was performed using the CellQuest Pro software.…”

Section: Methodsmentioning

confidence: 99%

Green synthesis, characterization, and anticancer activity of hyaluronan/zinc oxide nanocomposites

Namvar¹,

Azizi²,

Rahman³

et al. 2016

OTT

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The study describes an in situ green biosynthesis of zinc oxide nanocomposite using the seaweed Sargassum muticum water extract and hyaluronan biopolymer. The morphology and optical properties of the hyaluronan/zinc oxide (HA/ZnO) nanocomposite were determined by Fourier transform infrared spectroscopy, X-ray diffraction, field emission scanning electron microscopy, transmission electron microscopy, and ultraviolet–vis analysis. Electron microscopy and X-ray diffraction analysis showed that the zinc oxide nanoparticles were polydispersed with a mean size of 10.2±1.5 nm. The nanoparticles were mostly hexagonal in crystalline form. The HA/ZnO nanocomposite showed the absorption properties in the ultraviolet zone that is ascribed to the band gap of zinc oxide nanocomposite. In the cytotoxicity study, cancer cells, pancreatic adenocarcinoma (PANC-1), ovarian adenocarcinoma (CaOV-3), colonic adenocarcinoma (COLO205), and acute promyelocytic leukemia (HL-60) cells were treated with HA/ZnO nanocomposite. At 72 hours of treatment, the half maximal inhibitory concentration (IC50) value via the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was 10.8±0.3 μg/mL, 15.4±1.2 μg/mL, 12.1±0.9 μg/mL, and 6.25±0.5 μg/mL for the PANC-1, CaOV-3, COLO-205, and HL-60 cells, respectively, showing that the composite is most toxic to the HL-60 cells. On the other hand, HA/ZnO nanocomposite treatment for 72 hours did not cause toxicity to the normal human lung fibroblast (MRC-5) cell line. Using fluorescent dyes and flow cytometry analysis, HA/ZnO nanocomposite caused G2/M cell cycle arrest and stimulated apoptosis-related increase in caspase-3 and -7 activities of the HL-60 cells. Thus, the study shows that the HA/ZnO nanocomposite produced through green synthesis has great potential to be developed into an efficacious therapeutic agent for cancers.

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“…More examples of compounds loaded NLC are presented in Table 1. [31][32][33][34][35][36][37] Nanoemulsion (NE) It refers to an optically single isotropic and thermodynamic stable transparent (translucent) nonhomogeneous colloidal dispersion system ( Figure 2) with a droplet size Rapidly and well absorbed, and its relative bioavailability was improved more than 3-fold as compared with that of the puerarin suspensions with increased tissue concentrations in targeted organs, particularly the heart and brain.…”

Section: Nanocarriermentioning

confidence: 99%

<p>Novel Drug Delivery Systems for Loading of Natural Plant Extracts and Their Biomedical Applications</p>

Rahman

Othman

Hammadi

et al. 2020

IJN

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156

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Many types of research have distinctly addressed the efficacy of natural plant metabolites used for human consumption both in cell culture and preclinical animal model systems. However, these in vitro and in vivo effects have not been able to be translated for clinical use because of several factors such as inefficient systemic delivery and bioavailability of promising agents that significantly contribute to this disconnection. Over the past decades, extraordinary advances have been made successfully on the development of novel drug delivery systems for encapsulation of plant active metabolites including organic, inorganic and hybrid nanoparticles. The advanced formulas are confirmed to have extraordinary benefits over conventional and previously used systems in the manner of solubility, bioavailability, toxicity, pharmacological activity, stability, distribution, sustained delivery, and both physical and chemical degradation. The current review highlights the development of novel nanocarrier for plant active compounds, their method of preparation, type of active ingredients, and their biomedical applications.

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Comparison of Apoptotic Inducing Effect of Zerumbone and Zerumbone‐Loaded Nanostructured Lipid Carrier on Human Mammary Adenocarcinoma MDA‐MB‐231 Cell Line

Cited by 12 publications

References 22 publications

Zerumbone-Loaded Nanostructured Lipid Carrier Induces Apoptosis of Canine Mammary Adenocarcinoma Cells

Zerumbone-Loaded Nanostructured Lipid Carrier Induces Apoptosis of Canine Mammary Adenocarcinoma Cells

Green synthesis, characterization, and anticancer activity of hyaluronan/zinc oxide nanocomposites

<p>Novel Drug Delivery Systems for Loading of Natural Plant Extracts and Their Biomedical Applications</p>

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