Comparison of Basal-Cell Specific Markers – P63 and 34betae12 in Prostatic Glandular Proliferations

Abstract

Background: The prostate is a retroperitoneal organ encircling the neck of the bladder and urethra. Though the diagnosis of the prostatic lesions are analyzed through histopathological examination (HPE), sometimes, diagnosis can be challenging, when pathologist are faced with certain problems such as small foci of Ca or benign mimickers. In such situation, immunohistochemical (IHC) detection of basal cells are widely used. To Objectives: assess the expression of basal cell markers (p63 and 34betaE12) in various prostatic glandular proliferations and to differentiate suspicious glandular lesions as benign or malignant. A two year cross-sectional s Methods: tudy (Sept'2016 –Aug'2018) , total of 52 cases of both TURP and prostate biopsy specimens sent to the department of Pathology, RIMS for HPE were studied using IHC markers p63 and 34betaE12, following H&E stain and the expressions of the markers were studied. Results: Out of 52 cases, 41(78.8%) cases were diagnosed as Benign proliferative hyperplasia (BPH), 8(15.4%) cases as prostatic carcinoma, 2(3.8%) cases as high grade prostatic intraepithelial neoplasia (HGPIN) and one (1.9%) case of adenoleiomyobromatous hyperplasia (AMFH) on H&E section with age range of 51 to 90 years (mean age: 72 years). Following IHC staining, 43 (97.7%) benign cases were positive for both p63 and 34betaE12, one (2.3%) case of benign lesion was negative for both the IHCs. 8(100%) cases of malignant lesions were negative for both the IHCs. A p-value of 1.000 was observed indicating that there is no signicant difference in the sensitivity of p63 and 34betaE12. In this cross- Conclusion: sectional study of 52 cases of prostatic lesions, HPE and the role of basal cell specic IHC markers p63 and 34beta12 were studied. No signicant difference was observed in the sensitivity between the two markers. Further comparative study with larger sample size is needed to comprehend the differences in the utility of p63 and 34etaE12 in diagnosing suspicious prostatic lesions.