“…In addition, it was also observed from Fig. 6 that low molecular weight benzene derivatives were better removed by strain C1 than the high molecular weight benzene derivatives according to their relative abundance, in which the point was also supported by Genov et al ( 2008 ). Fig.…”
A potential degrader of paraffinic and aromatic hydrocarbons was isolated from oil-contaminated soil from steel plant effluent area in Burnpur, India. The strain was investigated for degradation of waste lubricants (waste engine oil and waste transformer oil) that often contain EPA (Environmental Protection Agency, USA) classified priority pollutants and was identified as Ochrobactrum sp. C1 by 16S rRNA gene sequencing. The strain C1 was found to tolerate unusually high waste lubricant concentration along with emulsification capability of the culture broth, and its degradation efficiency was 48.5 ± 0.5 % for waste engine oil and 30.47 ± 0.25 % for waste transformer oil during 7 days incubation period. In order to get optimal degradation efficiency, a three level Box–Behnken design was employed to optimize the physical parameters namely pH, temperature and waste oil concentration. The results indicate that at temperature 36.4 °C, pH 7.3 and with 4.6 % (v/v) oil concentration, the percentage degradation of waste engine oil will be 57 % within 7 days. At this optimized condition, the experimental values (56.7 ± 0.25 %) are in a good agreement with the predicted values with a calculated R2 to be 0.998 and significant correlation between biodegradation and emulsification activity (E24 = 69.42 ± 0.32 %) of the culture broth toward engine oil was found with a correlation coefficient of 0.972. This is the first study showing that an Ochrobactrum sp. strain is capable of degrading waste lubricants, which might contribute to the bioremediation of waste lubricating oil-contaminated soil.
“…In addition, it was also observed from Fig. 6 that low molecular weight benzene derivatives were better removed by strain C1 than the high molecular weight benzene derivatives according to their relative abundance, in which the point was also supported by Genov et al ( 2008 ). Fig.…”
A potential degrader of paraffinic and aromatic hydrocarbons was isolated from oil-contaminated soil from steel plant effluent area in Burnpur, India. The strain was investigated for degradation of waste lubricants (waste engine oil and waste transformer oil) that often contain EPA (Environmental Protection Agency, USA) classified priority pollutants and was identified as Ochrobactrum sp. C1 by 16S rRNA gene sequencing. The strain C1 was found to tolerate unusually high waste lubricant concentration along with emulsification capability of the culture broth, and its degradation efficiency was 48.5 ± 0.5 % for waste engine oil and 30.47 ± 0.25 % for waste transformer oil during 7 days incubation period. In order to get optimal degradation efficiency, a three level Box–Behnken design was employed to optimize the physical parameters namely pH, temperature and waste oil concentration. The results indicate that at temperature 36.4 °C, pH 7.3 and with 4.6 % (v/v) oil concentration, the percentage degradation of waste engine oil will be 57 % within 7 days. At this optimized condition, the experimental values (56.7 ± 0.25 %) are in a good agreement with the predicted values with a calculated R2 to be 0.998 and significant correlation between biodegradation and emulsification activity (E24 = 69.42 ± 0.32 %) of the culture broth toward engine oil was found with a correlation coefficient of 0.972. This is the first study showing that an Ochrobactrum sp. strain is capable of degrading waste lubricants, which might contribute to the bioremediation of waste lubricating oil-contaminated soil.
“…Abbas et al (2008) concluded that the FTIR analysis indicates stages of degradation and structural modifications of compounds. Genov et al (2008), using the same method, reported different degrees of oil hydrocarbons degradation while Liao et al (2009), studying asphaltene, found that the absorption of C=O tends to decrease with increasing biodegradation.…”
The introduction of biodiesel to diesel may allow the fuel to be more susceptible to microorganism growth, especially during incorrect storage. To evaluate the effect of adding biodiesel in pure diesel on the growth of Paecilomyces variotii, microcosms containing pure diesel (B0), blend diesel/biodiesel (B7) and pure biodiesel (B100) were used. In microcosm with minimal mineral medium and B0, B7 or B100, after 60 days, the biomass (dry weight) formed at interface oil-water in B7 and B100 was significantly higher when compared to that of B0. Infrared analysis showed reduction of the carbonile fraction in B7 and B100 suggesting formation of intermediate compounds in B7. To monitor possible contamination of fuel storage tank by P. variotii samples were collected and analysed by specific-PCR assay for detection of P. variotii spores in the aqueous phase. This method was able to detect a minimum of 10 3 spores ml -1 , corresponding to 0.0144 ng µl -1 of DNA. Specificity was tested against Aspergillus fumigatus and Pseudallescheria boydii.Keywords: biodiesel, Paecilomyces variotii, deteriogenic, biodegradation, infrared spectroscopy, PCR.Crescimento de Paecilomyces variotii em B0 (diesel), B100 (biodiesel) e B7 (mistura), degradação e detecção molecular Resumo A introdução de biodiesel ao diesel pode permitir que o combustível se torne mais suscetível ao crescimento de microorganismos, especialmente durante o armazenamento incorreto. Para analisar o efeito da adição de biodiesel em diesel puro no crescimento de Paecilomyces variotii, avaliou-se seu desenvolvimento em microcosmos contendo diesel puro (B0), mistura diesel/biodiesel (B7) e biodiesel puro (B100). Em microcosmos com meio mineral mínimo e B0, B7 ou B100, após 60 dias, a biomassa (peso seco) formada na interface óleo-agua com B7 e B100 foi significativamente maior quando comparada com a de B0. A análise de infravermelho mostrou redução da fração carbonila em B7 e B100, sugerindo a formação de compostos intermediários em B7. Para monitorar uma possível contaminação de tanque de armazenamento de combustível por P. variotii, amostras foram colhidas e analisadas por um teste de PCR específico para detecção de esporos deste fungo em fase aquosa. Este método foi capaz de detectar um mínimo de 10 3 esporos ml -1, correspondente a 0.0144 ng µl -1 de DNA. Especificidade foi testada contra Aspergillus fumigatus e Pseudallescheria boydii.Palavras-chave: biodiesel, Paecilomyces variotii, deteriogênico, biodegradação, espectroscopia de infravermelho, PCR.
“…4.4). Infrared (IR) spectroscopy coupled with multivariate analyses have been shown to be a useful non-destructive method for studying biodegradation of crude oil (Genov et al 2008).…”
Section: How To Get Information About Biodegradation Of Oil Fieldsmentioning
The concentrations of organic acids in produced water are highly variable. Because of the information shown in others chapters of this book about the toxic effects of organic acids, it is of major interest to understand the mechanisms controlling the occurrence of this compound group in produced water. This chapter focuses on in-reservoir biogeochemical processes which may produce organic acids as products or by-products. The biodegradation processes inside reservoirs decrease the hydrocarbon content of petroleum. Additionally, an increase in oil acidity as measured by total acid number (TAN) is frequently observed. Since the formation waters of reservoirs are in close contact with these processes, the production of smaller, more polar petroleum constituents will also have an effect on the composition of organic molecules in the produced water. This manuscript reviews the literature with respect to acids production of petroleum biodegradation and the effects of water re-injection on such processes.
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