Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells

Abstract

Background: Bone marrow-derived mesenchymal stem cells (BM-MSCs) and adipose tissue-derived mesenchymal stem cells (AT-MSCs) are potential cellular sources of therapeutic stem cells. MSCs are a multipotent population of cells capable of differentiating into a number of mesodermal lineages. Treatment using MSCs appears to be a helpful approach for structural restoration in regenerative medicine. Correct identification of these cells is necessary, but there is inadequate information on the MSC profile of cell surface markers and mRNA expression in dogs. In this study, we performed molecular characterization of canine BM-MSCs and AT-MSCs using immunological and mRNA expression analysis.

A horse of a different color

“…This result agrees with the findings of others in equine MSCs (9), being coexpressed by more than the 92% of the cells (10). A high percentage of MSCs from other species such as canine, ovine, or porcine also display in their cell surface these molecules (11)(12)(13).…”

Comparative immunophenotyping of equine multipotent mesenchymal stromal cells: An approach toward a standardized definition

“…Therefore, one should consider the known differences in features of MSCs from different species. As in equine MSCs, CD 29 and CD 44 expressions are found consistently in canine (34,35,51,52), ovine (53), and porcine (54) MSCs. Corresponding to the equine antigen expression profile as well, CD 73 could not be detected in canine, rabbit, or sheep MSCs (35,55,56), while rat tendon derived, feline and bovine neonatal cell sources and caprine bone marrow-derived MSCs expressed this antigen (57)(58)(59)(60).…”

Treatment of osteoarthritis with mesenchymal stem cells

“…Most recently, characterization of canine A-MSCs and BM-MSCs identified that canine A-MSCs and BM-MSCs both demonstrated strong expression of CD29 and CD44, moderate expression of CD90, and were negative for CD34 and CD45. Oct3/4 and Sox2 were equally expressed in canine A-MSCs and BM-MSCs, while Nanog expression was 2.5-fold higher in A-MSCs than in BM-MSCs [52]. Markers of human A-MSCs include STRO-1, CD146, and 3G5, in which the 3G5-positive fraction demonstrated the greatest enrichment for CFU-F compared with the other sorted cell populations [53].…”