Comparison of Broiler Performance When Fed Diets Containing Event DP-3Ø5423-1, Nontransgenic Near-Isoline Control, or Commercial Reference Soybean Meal, Hulls, and Oil

Abstract

DP-3Ø5423-1 (305423) is a genetically modified soybean that was produced by biolistic insertion of the gm-fad2-1 gene fragment and gm-hra genes into the germline of soybean seeds. Expression of gm-fad2-1 results in greater concentrations of oleic acid (18:1) by suppressing expression of the endogenous FAD2-1 gene, which encodes an n-6 fatty acid desaturase enzyme that catalyzes desaturation of 18:1 to linoleic acid (18:2). The GM-HRA protein expressed by the gm-hra gene is a modified version of the soybean acetolactate synthase enzyme that is used as a selectable marker during transformation. A 42-d feeding trial was conducted with broiler chickens to compare the nutritional performance of 305423 soybeans with nontransgenic soybeans. Diets were prepared using processed fractions (meal, hulls, and oil) from 305423 soybean plants. For comparison, additional diets were produced with soybean fractions obtained from a nontransgenic near-isoline (control) and nontransgenic commercial Pioneer brand varieties (93B86, 93B15, and 93M40). Diets were fed to Ross x Cobb broilers (n = 120/group, 50% male and 50% female) in 3 phases. Starter, grower, and finisher diets contained 26.5, 23, and 21.5% soybean meal, respectively. Soybean hulls and oil were added at 1.0 and 0.5%, respectively, across all diets in each phase. No statistically significant differences were observed in growth performance (BW, mortality, feed efficiency), organ yield (liver and kidney), or carcass yield (breast, thigh, leg, wing, and abdominal fat) variables between broilers consuming diets prepared with isolated fractions from 305423 or near-isoline control soybean. Additionally, all performance and carcass variables from control and 305423 soybean treatment groups fell within tolerance intervals constructed for each response variable using data from broilers fed diets prepared with reference soybean fractions. Based on the results from this study, it was concluded that 305423 soybeans were nutritionally equivalent to non-transgenic control soybeans with a comparable genetic background.

Scientific Opinion on application EFSA‐GMO‐NL‐2007‐45 for the placing on the market of herbicide‐tolerant, high‐oleic acid, genetically modified soybean 305423 for food and feed uses, import and processing under Regulation (EC) No 1829/2003 from Pioneer

“…5.1.3.2. Chicken feeding study 28 The applicant provided a 42-day study, which was also described in a scientific publication (McNaughton et al, 2008). Six hundred commercial Ross Cobb broilers were assigned to five experimental groups (n = 60/sex/group, housed five per sex and per pen for a total of 12 pens per group) fed diets containing processed products, respectively, from soybean 305423 (test group), the negative segregant (control group) or commercial non-GM varieties (993B86, 93B15 and 93M40).…”

Prospects for the use of genetically modified crops with improved nutritional properties as feed materials in poultry nutrition

“…Such modifications can be particularly desirable for food applications, because the oil produced from the DP-3O5423-1 soybean has a better oxidative stability, which contributes to improved frying performance. The safety and nutritional value of processed meal, hulls and oil from 305423 soybean plants were determined in a feeding experiment conducted on broiler chickens (McNaughton et al, 2008). There were no significant differences in the body weight gain and feed efficiency, or in the mortality and organ carcass characteristics between the birds fed diets containing processed fractions from the 305423 soybeans and those fed diets using conventional soybeans.…”

“…For example, the DP-3O5423-1 soybean has been genetically modified by the insertion of the gm-fad2-1 gene fragment, which resulted in greater concentrations of oleic acid by suppressing the expression of the endogenous FAD2-1 gene, which encodes an n-6 fatty acid desaturase enzyme that catalyzes desaturation of 18:1 to linoleic acid (McNaughton et al, 2008). Such modifications can be particularly desirable for food applications, because the oil produced from the DP-3O5423-1 soybean has a better oxidative stability, which contributes to improved frying performance.…”

Challenges for Plant Breeders from the View of Animal Nutrition

“…Generally, such substances are mainly extracted from the crops and so-called co-products (e.g., soybean meal, rapeseed meal, and cotton seed meal; see [91]) could be available for animal nutrition. Except for the extracted nutrient(s), co-products do not vary strongly from their isogenic counterparts in the composition of main nutrients [156,157], but analyses are necessary for the composition of such co-products.…”