Comparison of cryoprotective methods for histological examination of rat and porcine lung tissue

Jarosova, Rea; Ondráčková, Petra; Patočka, Zdeněk; Sládek, Z.

doi:10.2754/avb202190020225

Cited by 6 publications

(1 citation statement)

References 17 publications

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“…During necropsy, samples from the lungs, brain and spleen were collected. Histology was performed as described previously [ 20 ]. Briefly, tissue samples were embedded into the cryoprotective medium and frozen in pre-cooled n-heptane (Penta, Praha, Czech Republic) placed on dry ice.…”

Section: Methodsmentioning

confidence: 99%

Vaccine against Streptococcus suis Infection in Pig Based on Alternative Carrier Protein Conjugate

et al. 2022

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Streptococcus suis is a serious pathogen in the pig industry with zoonotic potential. With respect to the current effort to reduce antibiotic use in animals, a prophylactic measure is needed to control the disease burden. Unfortunately, immunization against streptococcal pathogens is challenging due to nature of the interaction between the pathogen and the host immune system, but vaccines based on conjugates of capsular polysaccharide (CPS) and carrier protein were proved to be efficient. The main obstacle of these vaccines is manufacturing cost, limiting their use in animals. In this work, we tested an experimental vaccine against Streptococcus suis serotype 2 based on capsular polysaccharide conjugated to chicken ovalbumin (OVA) and compared its immunogenicity and protectivity with a vaccine based on CRM197 conjugate. Ovalbumin was selected as a cheap alternative to recombinant carrier proteins widely used in vaccines for human use. We found that the ovalbumin-based experimental vaccine successfully induced immune response in pigs, and the IgG antibody response was even higher than after immunization with capsular polysaccharideCRM197 conjugate. Protectivity of vaccination against infection was evaluated in the challenge experiment and was found promising for both conjugates.

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Section: Methodsmentioning

confidence: 99%

Vaccine against Streptococcus suis Infection in Pig Based on Alternative Carrier Protein Conjugate

et al. 2022

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Novel Applicator Utilizing HIFES and Enhanced Synchronized Radiofrequency+ for Subcutaneous Fat Reduction: Porcine Model Study

Robb,

Bernardy,

Jarosova

et al. 2024

Lasers Surg Med

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ObjectivesSubmental fullness has been associated with being perceived as unattractive. Technology combining radiofrequency and muscle stimulation offers submental contouring through fat reduction, muscle stimulation, and skin tightening. This study aims to demonstrate the effectiveness and safety of fat reduction aspect with a novel submentum applicator delivering HIFES and synchronized radiofrequency+ (RF+) energies.Materials and MethodsSix white pigs (sus scrofa domesticus, n = 6, 60–80 kg) were recruited for this study, five in the active group (n = 5) received four treatments on the abdominal area, one sow served as a control (n = 1). Ultrasound, histological, and RT‐qPCR methods were used as evaluation methods.ResultsFat thickness decreased at 1 month by −17.35% and at 2 month by 31.40%. Proapoptotic caspase‐9 gene expression increased (at 1 h, 6 h, 24 h to +43.45%, +21.22%, −8.36%), as well as caspase‐3 (+15.28%, +21.77%, −6.71%), while bcl2l1 activity decreased (−11.46% at 1 h, −17.02% at 6 h, −3.9% at 24 h). While the AI in the control animal had minimal change (at 1 h −0.08%, at 6 h −0.09%, and at 24 h −0.025%), the active group's AI increased from the baseline of 9.14 to 44.85 at 1 h (+391%), peaked at 6 h to 53.50 (+485%), and at 24 h to 38.17 (+318%).ConclusionThe study results indicate the efficacy and safety of subcutaneous fat reduction following the novel technology combining HIFES and RF+ energies, designed to target small localized areas.

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Assessing earthworm exposure to a multi-pharmaceutical mixture in soil: unveiling insights through LC–MS and MALDI-MS analyses, and impact of biochar on pharmaceutical bioavailability

Fučík,

Jarošová,

Baumeister

et al. 2024

Environ Sci Pollut Res

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In the European circular economy, agricultural practices introduce pharmaceutical (PhAC) residues into the terrestrial environment, posing a potential risk to earthworms. This study aimed to assess earthworm bioaccumulation factors (BAFs), the ecotoxicological effects of PhACs, the impact of biochar on PhAC bioavailability to earthworms, and their persistence in soil and investigate earthworm uptake mechanisms along with the spatial distribution of PhACs. Therefore, earthworms were exposed to contaminated soil for 21 days. The results revealed that BAFs ranged from 0.0216 to 0.329, with no significant ecotoxicological effects on earthworm weight or mortality (p > 0.05). Biochar significantly influenced the uptake of 14 PhACs on the first day (p < 0.05), with diminishing effects over time, and affected significantly the soil-degradation kinetics of 16 PhACs. Moreover, MALDI-MS analysis revealed that PhAC uptake occurs through both the dermal and oral pathways, as pharmaceuticals were distributed throughout the entire earthworm tissue without specific localization. In conclusion, this study suggests ineffective PhAC accumulation in earthworms, highlights the influence of biochar on PhAC degradation rates in soil, and suggests that uptake can occur through both earthworm skin and oral ingestion. Graphical Abstract

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Comparison of cryoprotective methods for histological examination of rat and porcine lung tissue

Cited by 6 publications

References 17 publications

Vaccine against Streptococcus suis Infection in Pig Based on Alternative Carrier Protein Conjugate

Vaccine against Streptococcus suis Infection in Pig Based on Alternative Carrier Protein Conjugate

Novel Applicator Utilizing HIFES and Enhanced Synchronized Radiofrequency+ for Subcutaneous Fat Reduction: Porcine Model Study

Assessing earthworm exposure to a multi-pharmaceutical mixture in soil: unveiling insights through LC–MS and MALDI-MS analyses, and impact of biochar on pharmaceutical bioavailability

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