Comparison of culture, single and multiplex real‐time PCR for detection of Sabin poliovirus shedding in recently vaccinated Indian children

Giri, Sidhartha; Rajan, Anand; Kumar, Nuthan; Dhanapal, Pavithra; Venkatesan, Jayalakshmi; Iturriza‐Gómara, Miren; Taniuchi, Mami; John, Jacob; Abraham, Asha Mary; Kang, Gagandeep

doi:10.1002/jmv.24793

Cited by 4 publications

(2 citation statements)

References 12 publications

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“…Therefore, virus isolation has been the standard method for poliovirus detection recommended by GPEI for many years. In the context of poliovirus containment, molecular methods for direct detection of PV from stool extracts without using cell culture were developed [57][58][59]. As long as polio is not eradicated worldwide, there is a risk for importation into polio-free regions [60][61][62][63][64][65].…”

Section: Discussionmentioning

confidence: 99%

Enterovirus Surveillance (EVSurv) in Germany

Keeren

Böttcher²,

Diedrich

2021

Microorganisms

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The major aim of the enterovirus surveillance (EVSurv) in Germany is to prove the absence of poliovirus circulation in the framework of the Global Polio Eradication Program (GPEI). Therefore, a free-of-charge enterovirus diagnostic is offered to all hospitals for patients with symptoms compatible with a polio infection. Within the quality proven laboratory network for enterovirus diagnostic (LaNED), stool and cerebrospinal fluid (CSF) samples from patients with suspected aseptic meningitis/encephalitis or acute flaccid paralysis (AFP) are screened for enterovirus (EV), typing is performed in all EV positive sample to exclude poliovirus infections. Since 2006, ≈200 hospitals from all 16 German federal states have participated annually. On average, 2500 samples (70% stool, 28% CSF) were tested every year. Overall, the majority of the patients studied are children <15 years. During the 15-year period, 53 different EV serotypes were detected. While EV-A71 was most frequently detected in infants, E30 dominated in older children and adults. Polioviruses were not detected. The German enterovirus surveillance allows monitoring of the circulation of clinically relevant serotypes resulting in continuous data about non-polio enterovirus epidemiology.

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Section: Discussionmentioning

confidence: 99%

Enterovirus Surveillance (EVSurv) in Germany

Keeren

Böttcher²,

Diedrich

2021

Microorganisms

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“…Seroconversion was defined as the detection of neutralizing antibodies at a dilution of ≥1:8. In a subset of 300 infants, shedding of type 3 poliovirus was assessed using real-time PCR in stool samples collected 7 days after vaccination [16].…”

Section: Methodsmentioning

confidence: 99%

Influence of Nonpolio Enteroviruses and the Bacterial Gut Microbiota on Oral Poliovirus Vaccine Response: A Study from South India

Praharaj

Parker

Giri

et al. 2018

The Journal of Infectious Diseases

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Time Taken to Detect and Respond to Polio Outbreaks in Africa and the Potential Impact of Direct Molecular Detection and Nanopore Sequencing

Shaw

Cooper

Gumede

et al. 2021

The Journal of Infectious Diseases

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Background Detection of poliovirus outbreaks relies on a complex laboratory algorithm of cell-culture, PCR and sequencing to distinguish wild-type and vaccine-derived polioviruses (VDPV) from Sabin-like strains. We investigated the potential for direct molecular detection and nanopore sequencing (DDNS) to accelerate poliovirus detection. Methods We analysed laboratory data for time required to analyse and sequence serotype-2 VDPV (VDPV2) in stool collected from children with acute flaccid paralysis in Africa (May 2016-February 2020). Impact of delayed detection on VDPV2 outbreak size was assessed through negative binomial regression. Results VDPV2 confirmation in 525 stools required a median of 49 days from paralysis onset (10th-90th percentile: 29-74), comprising collection and transport (median: 16 days), cell-culture (7 days), intratypic differentiation RT-qPCR (3 days) and sequencing (including shipping if required) (15 days). New VDPV2 outbreaks were confirmed a median of 35 days (27-60) after paralysis onset, which we estimate could be reduced to 16 days by DDNS (9-37). Because longer delays in confirmation and response were positively associated with more cases (p<0.001), we estimate that DDNS could reduce the number of VDPV2 cases before a response by 28% (95% CrI 12-42%). Conclusions DDNS could accelerate poliovirus outbreak response, reducing their size and the cost of eradication.

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Comparison of culture, single and multiplex real‐time PCR for detection of Sabin poliovirus shedding in recently vaccinated Indian children

Cited by 4 publications

References 12 publications

Enterovirus Surveillance (EVSurv) in Germany

Enterovirus Surveillance (EVSurv) in Germany

Influence of Nonpolio Enteroviruses and the Bacterial Gut Microbiota on Oral Poliovirus Vaccine Response: A Study from South India

Time Taken to Detect and Respond to Polio Outbreaks in Africa and the Potential Impact of Direct Molecular Detection and Nanopore Sequencing

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