Comparison of cytotoxic potency between freshly cultured and freshly thawed cytokine-induced killer cells from human umbilical cord blood

Ngo, Hieu Trong; Dang, Vy Thanh; Nguyen, Nguyen Ho-Thao; Bui, Anh Nguyen-Tu; Pham, Phuc Van

doi:10.1007/s10561-022-10022-8

Cited by 3 publications

(1 citation statement)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently it was reported that CIK cells can be cryopreserved with no significant loss of in vitro or in vivo cytotoxic potency [ 27 ]. Since treatment regimens for CIK therapy include multiple rounds of CIK infusion over many months [ 5 , 28 ], the ability to be able to use frozen aliquots from one large batch of CIK cells might be of practical benefit.…”

Section: Resultsmentioning

confidence: 99%

Generation and assessment of cytokine-induced killer cells for the treatment of colorectal cancer liver metastases

Li,

Tomita,

Dhakal

et al. 2024

Cancer Immunol Immunother

View full text Add to dashboard Cite

Colorectal cancer (CRC) is the second leading cause of cancer-related death worldwide. Cytokine-induced killer (CIK) cells are an adoptive immunotherapy reported to have strong anti-tumour activity across a range of cancers. They are a heterogeneous mix of lymphoid cells generated by culturing human peripheral blood mononuclear cells with cytokines and monoclonal antibodies in vitro. In this study, we investigated the yield and function of CIK cells generated from patients with CRC liver metastases. We first showed that CIK cells generated in serum free medium X-VIVO 15 were comparable to those from RPMI medium with 10% FBS in terms of the number and percentages of the main subsets of cells in the CIK culture, and the intracellular levels of granzyme B and perforin, and the pro-inflammatory cytokines IL-2, IFN-γ and TNF-α. The CIK cells were cytotoxic to CRC cell lines grown in 2D cultures or as spheroids, and against autologous patient-derived tumour organoids. Donor attributes such as age, sex, or prior chemotherapy exposure had no significant impact on CIK cell numbers or function. These results suggest that functional CIK cells can be generated from patients with CRC liver metastatic disease, and support further investigations into the therapeutic application of autologous CIK cells in the management of patients with CRC liver metastases.

show abstract

Section: Resultsmentioning

confidence: 99%

Generation and assessment of cytokine-induced killer cells for the treatment of colorectal cancer liver metastases

Li,

Tomita,

Dhakal

et al. 2024

Cancer Immunol Immunother

View full text Add to dashboard Cite

show abstract

Generation and assessment of cytokine-induced killer cells for the treatment of colorectal cancer liver metastases

Ying Li,

Tomita,

Dhakal

et al. 2023

Preprint

View full text Add to dashboard Cite

Colorectal cancer (CRC) is the second leading cause of cancer-related death worldwide. Cytokine-induced killer (CIK) cells are an adoptive immunotherapy reported to have strong anti-tumour activity across a range of cancers. They are a heterogeneous mix of lymphoid cells generated by culturing human peripheral blood mononuclear cells (PBMCs) with cytokines and monoclonal antibodies in vitro. We compared CIK cells from healthy donors and donors with CRC liver metastases, generated in RPMI supplemented with 10% fetal bovine serum (FBS) or three different serum-free media. CIK cells generated in serum free medium X-VIVO 15 were comparable to those from RPMI medium with 10% FBS in terms of the number and percentages of the main subsets of cells in the CIK culture, and the intracellular levels of granzyme B and perforin, and the pro-inflammatory cytokines IL-2, IFN-γ and TNF-α. There was no significant difference in cytotoxicity against CRC cell lines grown in 2D cultures or as spheroids, and against autologous patient-derived tumour organoids. CIK cells from patients with CRC liver metastases grown in X-VIVO 15 media were similar to those from healthy donors in each of the measures. Donor attributes such as age, sex, or prior chemotherapy exposure had no significant impact on CIK cell numbers or function. These results support further investigations into the therapeutic application of autologous CIK cells in the management of patients with CRC liver metastases.

show abstract

A gene regulatory network–aware graph learning method for cell identity annotation in single-cell RNA-seq data

Zhao,

Li,

Liu

et al. 2024

Genome Res.

View full text Add to dashboard Cite

Cell identity annotation for single-cell transcriptome data is a crucial process for constructing cell atlases, unraveling pathogenesis, and inspiring therapeutic approaches. Currently, the efficacy of existing methodologies is contingent upon specific data sets. Nevertheless, such data are often sourced from various batches, sequencing technologies, tissues, and even species. Notably, the gene regulatory relationship remains unaffected by the aforementioned factors, highlighting the extensive gene interactions within organisms. Therefore, we propose scHGR, an automated annotation tool designed to leverage gene regulatory relationships in constructing gene-mediated cell communication graphs for single-cell transcriptome data. This strategy helps reduce noise from diverse data sources while establishing distant cellular connections, yielding valuable biological insights. Experiments involving 22 scenarios demonstrate that scHGR precisely and consistently annotates cell identities, benchmarked against state-of-the-art methods. Crucially, scHGR uncovers novel subtypes within peripheral blood mononuclear cells, specifically from CD4+T cells and cytotoxic T cells. Furthermore, by characterizing a cell atlas comprising 56 cell types for COVID-19 patients, scHGR identifies vital factors like IL1 and calcium ions, offering insights for targeted therapeutic interventions.

show abstract

Comparison of cytotoxic potency between freshly cultured and freshly thawed cytokine-induced killer cells from human umbilical cord blood

Cited by 3 publications

References 46 publications

Generation and assessment of cytokine-induced killer cells for the treatment of colorectal cancer liver metastases

Generation and assessment of cytokine-induced killer cells for the treatment of colorectal cancer liver metastases

Generation and assessment of cytokine-induced killer cells for the treatment of colorectal cancer liver metastases

A gene regulatory network–aware graph learning method for cell identity annotation in single-cell RNA-seq data

Contact Info

Product

Resources

About