Comparison of detection methods for adenovirus from enteric clinical specimens

Ahluwalia, Gurmukh S.; Scott-Taylor, Tim H.; Klisko, Brian; Hammond, Gregory

doi:10.1016/0732-8893(94)90086-8

Cited by 18 publications

(10 citation statements)

References 25 publications

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“…Only in one study performed in Guatemala, it was reported that enteric adenovirus was seen 3 times more frequently than rotavirus and this has been linked to climate variations (5). Ahluwalia et al (6) stated that the prevalence of childhood gastroenteritis caused by adenovirus was 4.1%. In their studies done in our country by Gül et al (7), and Biçer et al (8), the incidence of gastroenteritis caused by adenovirus was found to be 4.7% and 16.2%, respectively.…”

Section: Discussionmentioning

confidence: 99%

Investigation of the Enteric Adenovirus Antigen Frequency by Immunochromotographic Method in Children with Acute Gastroenteritis

Akpınar¹,

Akpınar²

2017

meandros

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Objective: Gastroenteritis is the third common cause of death due to infections. After rotavirus, adenoviruses are also one of the reasons frequently seen in gastroenteritis in infants and children. This study is performed to determine the incidence of enteric virus serotype 40 and 41 in children with acute gastroenteritis in order to enable prompt and appropriate treatment. MaterialsandMethods:Stool specimens of patients who attended our clinic with a diagnosis of acute gastroenteritis between January 2013 and December 2013 were examined for the presence of enteric adenovirus (Ad40 and Ad41) antigen using immunochromatographic methods. Results: One hundred and two stool samples from 3206 were positive for adenovirus antigens. Adenovirus antigen positive-patients aged 0-5 years constituted 82.3% of patients. Adenovirus infections were observed in all seasons of the year. Conclusion: In our country, the epidemiology of adenovirus infection is not known very well. According to the data we obtained from the results of this study, we assume that idntifying viral agent in patients with diarrhea in an accurate, prompt and reliable way can prevent unnecessary antibiotic use and can contribute seroepidemiologic data in childhood gastroenteritis in our region. Amaç:Gastroenteritler enfeksiyonlara bağlı ölümler arasında ilk üç sırada yer almaktadır. Bebekler ve çocuklardaki gastroenteritlerin rotavirüslerden sonra diğer sık görülen nedenlerinden biri de adenovirüslerdir. Bu çalışma, akut gastroenterit nedeniyle başvuran çocuk hastalarda enterik adenovirüs serotip 40 ve 41 insidansını inceleyerek uygun tedaviye kısa sürede başlanabilmesi amacıyla yapılmıştır. Gereç ve Yöntemler: Ocak 2013-Aralık 2013 tarihleri arasında, akut gastroenterit tanısıyla başvuran 0-14 yaş grubu hastaların dışkı örneğinde enterik adenovirüs (Ad40 ve Ad41) antijen varlığı immünokromatografik yöntem kullanılarak araştırıldı. Bulgular: Üç bin ikiyüzaltı dışkı örneğinden 102'sinde (%3,2) adenovirüs antijenleri saptandı. Sıfır-beş yaş arası grup olguların %82,3'ünü oluşturmaktaydı. Adenovirüs enfeksiyonu yılın her mevsiminde görülmüştür. Sonuç: Ülkemizde adenovirüs enfeksiyonu seroepidemiyolojisi çok iyi bilinmemektedir. Yaptığımız bu çalışma sonucu elde ettiğimiz verileri değerlendirdiğimizde ishal şikayeti ile

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Section: Discussionmentioning

confidence: 99%

Investigation of the Enteric Adenovirus Antigen Frequency by Immunochromotographic Method in Children with Acute Gastroenteritis

Akpınar¹,

Akpınar²

2017

meandros

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“…Direct immunofluorescence and electron microscopy of respiratory secretion or tissue samples may be used to diagnose end-organ involvement [3]. Rapid testing of ocular, respiratory, and stool specimens may be performed with commercially available EIAs, although the sensitivity of these tests is limited [59].…”

Section: Diagnosis and Monitoringmentioning

confidence: 99%

Emerging Infections: Adenovirus Infections in Transplant Recipients

2006

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Adenoviruses are increasingly recognized as contributors to morbidity and mortality among stem cell and solid-organ transplant recipients. Clinical presentations range from asymptomatic viremia to respiratory and gastrointestinal disease, hemorrhagic cystitis, and severe disseminated illness. The limited clinical data available support the use of cidofovir for many of these illnesses. Prospective studies are needed to better understand the pathogenesis of and therapeutic options for adenoviral infections in this patient population.

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“…Değişik ülkelerde yapılan çalışmalarda adenovirusların viral gastroenterit olgularının %2.4-22.2'sinden sorumlu olduğu bildirilirken (25)(26)(27)(28)30,31), bu oran rotaviruslar için %11-71 olarak saptanmıştır (28,29).…”

Section: İrdelemeunclassified

Frequency of Adenovirus and Rotavirus and Their Seasonal Distribution in Children With Gastroenteritis

Tüzüner¹,

Gülcen²,

Özdemir³

et al. 2016

Klimik Dergisi

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Objective: In our study, we aimed to investigate the prevalence of rotavirus and enteric adenovirus in stool samples sent to our laboratory for antigen detection of children between 0-18 years of age admitted to hospital with diarrhea, abdominal pain, vomiting and fever and diagnosed as gastroenteritis. We also analyzed their frequencies according to demographic parameters. Methods: Results of 5156 pediatric patients admitted to Necmettin Erbakan University Meram Faculty of Medicine Hospital and diagnosed as gastroenteritis between January 2013-December 2015 were investigated retrospectively. VIKIA ® RotaAdeno (bioMérieux, Marcy l'Etoile, France), a chromatographic immunoassay detecting both viruses simultaneously was used according to the manufacturer's recommendations in stool samples. Results: Viral antigens were detected in 884 (17.1%) of the total 5156 samples. 764 (14.8%) of the positive results were detected as rotavirus and 120 (2.3%) were detected as adenovirus. Of the patients with positive results, 412 (46.6%) were female and 472 (53.4%) were male. When results are considered according to age, 2-4 age group was found to have the most common positivity (n=372) as 42.1%. Seasonal distribution of acute gastroenteritis cases was analyzed and the number of cases due to rotavirus was found to be increased in winter and spring and enteric adenoviruses were detected all year round. Conclusions: Rotavirus is the most common reason of gastroenteritis in the newborn and children, which must be considered for patients with diarrhea especially in the first four years of life. Rapid diagnosis is important for prediction of clinical implications and treatment. As enteric adenovirus is an important reason of gastroenteritis in infancy and childhood, it is necessary to investigate adenovirus antigens as well. Conducting regional studies are important for contributing to epidemiological data. Klimik Dergisi 2016; 29(3): 121-4.

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Comparison of detection methods for adenovirus from enteric clinical specimens

Cited by 18 publications

References 25 publications

Investigation of the Enteric Adenovirus Antigen Frequency by Immunochromotographic Method in Children with Acute Gastroenteritis

Investigation of the Enteric Adenovirus Antigen Frequency by Immunochromotographic Method in Children with Acute Gastroenteritis

Emerging Infections: Adenovirus Infections in Transplant Recipients

Frequency of Adenovirus and Rotavirus and Their Seasonal Distribution in Children With Gastroenteritis

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