Comparison of different cationic polymers efficacy in fabrication of alginate multilayer microcapsules

Hajifathaliha, Fariba; Mahboubi, Arash; Nematollahi, Leila; Mohit, Elham; Bolourchian, Noushin

doi:10.1016/j.ajps.2018.11.007

Cited by 23 publications

(15 citation statements)

References 45 publications

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“…14 ± 25.86 Linear poly ethylene imine and poly l-lysine in fabrication of cell-loaded alginate microcapsules Advanced Pharmaceutical Bulletin, 2020, Volume 10, Issue 2 293 (µm), respectively (n = 3). As stated in the previous study, 8 the images taken by the inverted microscope along with the SEM images of cALA and cAPA microcapsules clearly indicated that the electrostatic bead generator method along with the influential variable parameters such as the concentration of alginate solution, distance between the needle and the surface of CaCl 2 solution, extrusion rate, etc. were all well suited for fabrication of microcapsules with a similar size and shape and no marked differences (Figures 1 and 2).…”

Section: Physical Characteristicssupporting

confidence: 70%

“…The microcapsules were produced using electrostatic bead generator under conditions stated elsewhere. 8 Briefly, using electrostatic bead generator (flow rate: 15 mL/h, applied voltage: 10 kV), 5 mL of cell containing sodium alginate solution was extruded into the 120 mM CaCl 2 solution. The resultant cell loaded microcapsules were allowed to remain in CaCl 2 solution for 15 minutes on a magnetic stirrer.…”

Section: Chok 1 Growth Curvementioning

confidence: 99%

“…All the experiments were performed in triplicates with the results reported as mean ± SD. 8,11 Surface morphology: SEM studies The surface morphology of the microcapsules was studied using scanning electron microscope (SEM). Freezedried microcapsules were fixed on conducting stubs and vacuum coated with gold palladium film using a sputter coater (Edward S-150, UK).…”

Section: Characterization Of the Microcapsules Shape And Sizementioning

confidence: 99%

“…Enlargement of microcapsules size is inversely proportional to their stability. 8,13 The experiments were conducted three times, with the results reported as mean ± SD.…”

Section: Long-term Stability Of the Microcapsulesmentioning

confidence: 99%

“…In our previous study, to ensure the similar performance of LPEI and PLL, the physical properties, surface morphology, mechanical strength, cytotoxicity, and long-term stability of ALA and APA microcapsules were investigated. 8 Since the ALA microcapsules showed similar results to APA in all the physico-mechanical and cytotoxicity evaluations, it could be employed in further comparative investigations. The purpose of this study was to finalize the comparison between PLL and LPEI as well as evaluation of these two polymers for the encapsulation of CHOK 1 ; the cell line which has attracted a great deal of attention in manufacturing of biopharmaceuticals.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Linear Poly Ethylene Imine (LPEI) and Poly L-Lysine (PLL) in Fabrication of CHOK1 Cell-Loaded Multilayer Alginate Microcapsules

et al. 2020

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Purpose: Poly l-lysine (PLL) has been introduced as a strengthening covering layer for alginate microcapsules which are the most convenient way for cell encapsulation. Some disadvantages of PLL such as high price and low biocompatibility have prompted scientists to find better alternatives. Linear poly ethylene imine (LPEI), thanks to its highly similar structure to PLL, could be considered as a proper cost-effective alternative. In this study LPEI and PLL were compared as covering layers of cell-loaded alginate-LPEI-alginate (cALA) and alginate-PLL-alginate (cAPA) microcapsules. Methods: In addition to the physico-mechanical properties, the encapsulation efficiency, cell survival post encapsulation, cell viability, and cellular metabolic activity within the microcapsules were evaluated using trypan blue, live/dead cell staining, and MTT test, respectively. Results: Physico-mechanical evaluation of the microcapsules revealed that the cell microencapsulation process did not affect their shape, size, and mechanical stability. Although the encapsulation efficiency for cALA and cAPA was not different (P>0.05), cell survival post encapsulation was higher in cALA than in cAPA (P<0.05) which could be the reason for the higher cell viability and also cellular metabolic activity within these microcapsules in comparison to cAPA. Conclusion: Here, based on these results, ALA could be introduced as a preferable alternative to APA for cell encapsulation.

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Section: Physical Characteristicssupporting

confidence: 70%

Section: Chok 1 Growth Curvementioning

confidence: 99%

Section: Characterization Of the Microcapsules Shape And Sizementioning

confidence: 99%

“…Enlargement of microcapsules size is inversely proportional to their stability. 8,13 The experiments were conducted three times, with the results reported as mean ± SD.…”

Section: Long-term Stability Of the Microcapsulesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparison of Linear Poly Ethylene Imine (LPEI) and Poly L-Lysine (PLL) in Fabrication of CHOK1 Cell-Loaded Multilayer Alginate Microcapsules

et al. 2020

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Encapsulation Technologies of Plant Growth‐Promoting Microorganisms and Microbial Biological Control Agents Against Plant Pathogenic Organisms

Marcial-Coba

Soria‐Delgado

Yánez‐Altuna

2021

Annual Plant Reviews Online

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As a function of the global necessity to reduce or replace the use of chemical fertilisers and pesticides, due to their toxicity, the formulation of microbial inoculants has been suggested as an environmentally friendly alternative for maintaining crop production at economically sustainable levels. In this connection, the microencapsulation of beneficial soil‐borne microorganisms is conceived as a strategy to improve the inoculant's efficiency, in terms of viability and functionality. In this article, we describe the most widely applied microencapsulation techniques, including the mechanisms and wall materials involved, as well as the advantages and shortcomings of using different encapsulation technologies. Moreover, the desiccation technologies used as a tool to extend the shelf‐life of immobilised microbes are also explained. Finally, the outcomes of various, relatively recent studies regarding the encapsulation of plant growth‐promoting microorganisms and biocontrol agents, including bacteriophages, are discussed. This article may contribute to the design of microbial inoculant delivery strategies, aiming to increase crop production and to reduce the utilisation of chemical fertilisers and pesticides.

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Droplet Microfluidics Powered Hydrogel Microparticles for Stem Cell‐Mediated Biomedical Applications

Zheng,

Tian,

et al. 2024

Small

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Stem cell‐related therapeutic technologies have garnered significant attention of the research community for their multi‐faceted applications. To promote the therapeutic effects of stem cells, the strategies for cell microencapsulation in hydrogel microparticles have been widely explored, as the hydrogel microparticles have the potential to facilitate oxygen diffusion and nutrient transport alongside their ability to promote crucial cell‐cell and cell‐matrix interactions. Despite their significant promise, there is an acute shortage of automated, standardized, and reproducible platforms to further stem cell‐related research. Microfluidics offers an intriguing platform to produce stem cell‐laden hydrogel microparticles (SCHMs) owing to its ability to manipulate the fluids at the micrometer scale as well as precisely control the structure and composition of microparticles. In this review, the typical biomaterials and crosslinking methods for microfluidic encapsulation of stem cells as well as the progress in droplet‐based microfluidics for the fabrication of SCHMs are outlined. Moreover, the important biomedical applications of SCHMs are highlighted, including regenerative medicine, tissue engineering, scale‐up production of stem cells, and microenvironmental simulation for fundamental cell studies. Overall, microfluidics holds tremendous potential for enabling the production of diverse hydrogel microparticles and is worthy for various stem cell‐related biomedical applications.

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Comparison of different cationic polymers efficacy in fabrication of alginate multilayer microcapsules

Cited by 23 publications

References 45 publications

Comparison of Linear Poly Ethylene Imine (LPEI) and Poly L-Lysine (PLL) in Fabrication of CHOK1 Cell-Loaded Multilayer Alginate Microcapsules

Comparison of Linear Poly Ethylene Imine (LPEI) and Poly L-Lysine (PLL) in Fabrication of CHOK1 Cell-Loaded Multilayer Alginate Microcapsules

Encapsulation Technologies of Plant Growth‐Promoting Microorganisms and Microbial Biological Control Agents Against Plant Pathogenic Organisms

Droplet Microfluidics Powered Hydrogel Microparticles for Stem Cell‐Mediated Biomedical Applications

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