Comparison of Different Culture Methods for the Detection of <i>Bacillus cereus</i> Group in Cosmetics

Yossa, Nadine; Hoang, Son T.; Canida, Travis; Bell, Rebecca; Tallent, Sandra M.; Brown, Eric W.; Hammack, Thomas S.

doi:10.1093/jaoacint/qsaa016

Cited by 3 publications

(4 citation statements)

References 9 publications

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“…Staphylococcus epidermidis was isolated in powders, blushers 11 , and various eye cosmetics 11 , 34 , 35 . Bacillus cereus isolates were previously detected in various eye cosmetics 24 , lip glosses 16 and creams 15 . Bacillus spp.…”

Section: Discussionmentioning

confidence: 99%

“…have been identified 2 , 10 , 11 . In creams, Escherichia coli, Bacillus spp, Bacillus cereus, Klebsiella spp., Klebsiella pneumoniae, Pseudomonas spp., Staphylococcus spp., Enterobacter spp., Enterococcus faecalis, Micrococcus spp., Staphylococcus aureus, Streptococcus pyogenes, and Enterobacter aerogenes have been detected 2 , 8 , 10 , 12 – 15 . Another study has shown that Escherichia hermannii, S. aureus, Bacillus cereus, and Enterobacter species were isolated from lip glosses and lipsticks.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of potential pathogenic bacterial load and multidrug resistance in locally manufactured cosmetics commonly used in Dhaka metropolis

Nusrat

Zahra

Ahmed

et al. 2023

Sci Rep

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In Bangladesh cosmetics are being produced disregarding the Good Manufacturing Practices. So, this study aimed to test the level and nature of bacterial contamination of such cosmetics. A total of 27 cosmetics comprising eight lipsticks, nine powders, and ten creams were bought from New Market and Tejgaon areas of Dhaka city and tested. Bacteria was detected in 85.2% of samples. Majority of the samples (77.8%) exceeded the limit given by the Bangladesh Standards and Testing Institution (BSTI), Food and Drug Administration (FDA) and the International Organization for Standardization (ISO). Both Gram-negative (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Salmonella spp.) and Gram-positive bacteria (species of Streptococcus, Staphylococcus, Bacillus and Listeria monocytogenes) were identified. Hemolysis was observed in 66.7% Gram-positive and 25% Gram-negative bacteria. Multidrug resistance was tested in 165 randomly selected isolates. Every species of Gram-positive and Gram-negative bacteria exhibited varying levels of multidrug resistance. The highest levels of antibiotic resistance were in broad-spectrum antibiotics (ampicillin, azithromycin, cefepime, ciprofloxacin and meropenem) and narrow-spectrum Gram-negative antibiotics (aztreonam and colistin). Multidrug resistance was 12–78% in Gram-negative bacteria and 12–100% in Gram-positive bacteria. Coagulase and DNase were identified in 97.5% and 5.1% of Staphylococcus aureus isolates respectively. Our findings indicate that these cosmetics pose a risk to the public’s health.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Assessment of potential pathogenic bacterial load and multidrug resistance in locally manufactured cosmetics commonly used in Dhaka metropolis

Nusrat

Zahra

Ahmed

et al. 2023

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Staphylococcus epidermidis was isolated in powders, blushers 9 , and various eye cosmetics 9,29,30 . Bacillus cereus isolates were previously detected in various eye cosmetics 23 , lip glosses 14 and creams 13 . Bacillus spp.…”

Section: Discussionmentioning

confidence: 99%

“…have been identi ed 2,8,9 . In creams, Escherichia coli, Bacillus spp, Bacillus cereus, Klebsiella spp., Klebsiella pneumoniae, Pseudomonas spp., Staphylococcus spp., Enterobacter spp., Enterococcus faecalis, Micrococcus spp., Staphylococcus aureus, Streptococcus pyogenes, and Enterobacter aerogenes have been detected 2,6,8,[10][11][12][13] . Another study has shown that Escherichia hermannii, S. aureus, Bacillus cereus, and Enterobacter species were isolated from lip glosses and lipsticks.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Potential Pathogenic Bacterial Load and Multidrug Resistance in Locally Manufactured Cosmetics Commonly Used in Dhaka Metropolis

Nusrat

Zahra

Ahmed

et al. 2022

Preprint

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In Bangladesh cosmetics are being produced disregarding the safety rules. So, this study aimed to test the level and nature of bacterial contamination of such cosmetics. A total of 27 cosmetics comprising eight lipsticks, nine powders, and ten creams were bought from New Market and Tejgaon areas of Dhaka city and tested. Bacteria was detected in 85.2% of samples. Majority of the samples (77.8%) exceeded the limit given by the FDA (Food and Drug Administration) and EU (European Union). Both Gram-negative (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Salmonella spp.) and Gram-positive bacteria (species of Streptococcus, Staphylococcus, Bacillus and Listeria monocytogenes) were identified. Hemolysis was observed in 66.7% Gram-positive and 25% Gram-negative bacteria. Multidrug resistance was tested in 165 randomly selected isolates. Every species of Gram-positive and Gram-negative bacteria exhibited varying levels of multidrug resistance. The highest levels of antibiotic resistance were in broad-spectrum antibiotics (ampicillin, azithromycin, cefepime, ciprofloxacin and meropenem) and narrow-spectrum Gram-negative antibiotics (aztreonam and colistin). Multidrug resistance was 12%-78% in Gram-negative bacteria and 12%-100% in Gram-positive bacteria. Coagulase and DNase were identified in 97.5% and 5.1% of Staphylococcus aureus isolates respectively. Our findings indicate that these cosmetics pose a risk to the public's health.

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Soleris® Automated System for the Rapid Detection of Burkholderia cepacia Complex in Cosmetic Products

Zhang

Tolan

Lavigne

et al. 2022

Journal of AOAC INTERNATIONAL

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Background Burkholderia cepacia complex (Bcc) has emerged as an important opportunistic pathogen with rising concern in pharmaceuticals and cosmetic products. The Bcc supplement (S2-BCC-S) was purposely developed and used with the Pseudomonas vial (PD-109) for the detection of Bcc through the Soleris® Next Generation automated instrument system. Objective This study aimed to evaluate the performance of the Soleris® Bcc testing method for cosmetic products. Method Inclusivity and exclusivity were assessed with the Soleris Bcc method and United States Pharmacopeia (USP) method in three enrichment broths. Matrix testing was conducted using 28 cosmetic products to compare the equivalency of the Soleris Bcc method to that of the USP reference method. Repeatability of the Soleris Bcc assay, method robustness, product stability, and lot-to-lot consistency of the Soleris reagents were also assessed. Results Both the Soleris Bcc and the USP methods supported the growth of all 26 inclusivity strains, except the USP method missed one inclusivity strain in one broth. For exclusivity, 0–6% was presumptive positive with the Soleris Bcc method, and 42–48% was presumptive positive with the reference method. Kappa index was 0.96 for the matrix testing, indicating a good agreement between the Soleris Bcc assay and reference method for testing Bcc in cosmetics. Repeatability results showed the coefficient of variation was less than 4%. The robustness and ruggedness study yielded detection times within 1 h differences when small variations were introduced. The lot-to-lot study showed consistent results among four lots of the Bcc reagents. Conclusions The automated Soleris method was successfully demonstrated to be robust, sensitive, and specific for Bcc detection in cosmetic products. Highlights The Soleris Bcc method is user friendly. It shows the results in real-time and generates the report automatically. Implementation of this method for detection of Bcc in cosmetics would save significant time and resources.

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Comparison of Different Culture Methods for the Detection of Bacillus cereus Group in Cosmetics

Abstract: Abstract Background The U.S. Food and Drug Administration (FDA) Bacteriological Analytical Manual (BAM) reference culture method uses Modified Letheen Broth (MLB) for microbiological analyses for all types of cosmetic products. Objective This study evaluated the e… Show more

Cited by 3 publications

References 9 publications

Assessment of potential pathogenic bacterial load and multidrug resistance in locally manufactured cosmetics commonly used in Dhaka metropolis

Assessment of potential pathogenic bacterial load and multidrug resistance in locally manufactured cosmetics commonly used in Dhaka metropolis

Assessment of Potential Pathogenic Bacterial Load and Multidrug Resistance in Locally Manufactured Cosmetics Commonly Used in Dhaka Metropolis

Soleris® Automated System for the Rapid Detection of Burkholderia cepacia Complex in Cosmetic Products

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