Comparison of different enzyme‐linked immunosorbent assay methods for avidity determination of antiphospholipid antibodies

Fialová, L; Petráčková, Milada; Kuchar, O

doi:10.1002/jcla.22121

Cited by 23 publications

(26 citation statements)

References 31 publications

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“…The avidity index (AI) of anti-CSP antibodies was evaluated using an ELISA-based chaotrope avidity assay 52 . This protocol followed the standard ELISA (described above), except that following serum incubation wells were treated with 6M urea for 10 minutes.…”

Section: Measurement Of Antibody Aviditymentioning

confidence: 99%

An Epitope-based Malaria Vaccine Targeting the Junctional Domain of Circumsporozoite Protein

Jelínková

Jhun

Eaton

et al. 2020

Preprint

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A malaria vaccine that elicits long-lasting protection and is suitable for use in endemic areas remains urgently needed. Here, we assessed the immunogenicity and prophylactic efficacy of a vaccine targeting a recently described epitope on the major surface antigen on Plasmodium falciparum sporozoites, circumsporozoite protein (CSP). Using a virus-like particle (VLP)-based vaccine platform technology, we developed a vaccine that targets the junctional region between the N-terminal and central repeat domains of CSP. This region is recognized by monoclonal antibodies, including mAb CIS43, that have been shown to potently prevent liver invasion in animal models. We show that CIS43 VLPs elicit high titer and long-lived anti-CSP antibody responses in mice and non-human primates. Immunization with CIS43 VLPs confers partial protection from malaria infection in a mouse model, and both immunogenicity and protection were enhanced when mice were immunized with CIS43 VLPs in combination with adjuvants including delta inulin polysaccharide particles and TLR9 agonists. Passive transfer of serum from immunized macaques also inhibited parasite liver invasion in the mouse infection model. Our findings demonstrate that a Qβ VLP-based vaccine targeting the CIS43 epitope combined with various adjuvants is highly immunogenic in mice and macaques, elicits long-lasting anti-CSP antibodies, and inhibits parasite infection in a mouse model. Thus, the CIS43 VLP vaccine is a promising pre-erythrocytic malaria vaccine candidate.

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Section: Measurement Of Antibody Aviditymentioning

confidence: 99%

An Epitope-based Malaria Vaccine Targeting the Junctional Domain of Circumsporozoite Protein

Jelínková

Jhun

Eaton

et al. 2020

Preprint

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“…A variety of methods and their modification for antibody avidity has already been described [11,30]. Recently, we explored in detail aCL IgG avidity methods using the principle of ELISA with chaotropes, which break the interactions of low-avidity antibodies with antigens [18]. A higher concentration of chaotropic agents caused more effective disruption of immune complexes.…”

Section: Discussionmentioning

confidence: 99%

“…We found that both urea in concentrations of 6 mol/l or 8 mol/l and sodium chloride in concentrations of 1 mol/l or 2 mol/l were suitable for the sufficient dissociation of immune complexes during an extra step in the ELISA procedure. The extended description of the ELISA for avidity examination using chaotropic agents is shown in Fialová et al [17,18]. For the purpose of this study, we used sera diluted 1:50 and urea in concentrations of 6 mol/l and 8 mol/l as a chaotrope.…”

Section: Avidity Of Acl Iggmentioning

confidence: 99%

“…Antibody avidities used to be examined in the antibodies directed against infectious agents, with the aim to evaluate their avidity maturation associated with the increase of specific humoral immunity [12,13]. Avidities of autoantibodies including aPLs have already been investigated [14][15][16][17][18][19]. In contrast to anti-infectious antibodies, the autoantibody avidities can reach different values.…”

Section: Introductionmentioning

confidence: 99%

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Avidity of anti-phospholipid antibodies in relation to their levels

Fialová

Kuchar

Petráčková

et al. 2020

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Introduction: the heterogeneity of anti-phospholipid antibodies can be manifested not only in different antigenic specificities, but also in their avidities. the aim of the study was to investigate the relationship between anti-cardiolipin antibody (acL) igG avidities and levels within the range of their titres, from very low to high ones. Material and methods: We analyzed 78 serum samples from 60 patients by eLiSa with chaotropic agents, using urea concentration of 6 and 8 mol/l and single diluted serum samples. the changes of acL levels and avidities were explored during a long-term follow-up in 14 patients. Results: the avidities of acLs did not differ in the groups of patients classified according to acL levels. the higher avidity antibodies predominated in our patients and the fluctuation of avidities in the longitudinal follow-up did not show significant differences. no relationship between acL levels and their avidities was found. Conclusions: acL avidities seem to have no relationship with acL levels and high-avidity acLs; the potentially deleterious effects might be present also in patients with low and extremely low acL levels. avidity of acLs belongs to stable characteristics with insignificant changes in time.

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“…La urea es un agente caotrópico que afecta los enlaces de hidrógeno, desestabilizando las fuerzas hidrófobas que actúan en la unión antí genoanticuerpo. Las uniones débiles se rompen, quedando unidas las que tienen mayor afinidad funcional o avidez (Fialová et al, 2017). Este efecto es similar al logrado cuando los sueros son absorbidos con otras micobacterias, como Myco bacterium phlei (Di Paolo et al, 2016; OIE, 2018.…”

Section: Introductionunclassified

Seroprevalencia de paratuberculosis bovina mediante la prueba de ELISA urea en rodeos de cría y de leche con sospecha de la enfermedad, localizados en la provincia de Buenos Aires, Argentina

Pinedo

Paolo

Sosa

et al. 2019

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Se presentan los resultados obtenidos en 17.747 sueros analizados para el diagnóstico serológico de paratuberculosis bovina, provenientes de 85 rodeos de cría y 5 de leche, ubicados en 14 partidos de la provincia de Buenos Aires. La prueba de ELISA permite diagnosticar esta enfermedad en estadio subclínico, y la incorporación de urea mejora la eficiencia para detectar los anticuerpos específicos contra el Mycobacterium avium subsp. paratuberculosis (MAP). Se estimó la seroprevalencia sobre la base de los resultados obtenidos de animales caracterizados como positivos y sospechosos en la prueba de ELISA urea. Se observó una seroprevalencia individual del 5,5 % en los rodeos de cría y del 13,6 % en los rodeos lecheros, siendo la seroprevalencia predial del 78,8 %. De 425 muestras de heces cultivadas se aisló MAP en el 67,6 % de los animales positivos, del 52,7 % de los sospechosos y del 7 % de los negativos por la prueba de ELISA urea. Mediante estadística bayesiana, el valor predictivo positivo de esta prueba fue de 0,61 y el valor predictivo negativo de 0,93. Nuestros resultados aportan información actualizada sobre la estimación de la seroprevalencia regional de paratuberculosis bovina, la cual es elevada, y confirma la utilidad de la prueba de ELISA urea, desarrollada localmente, en el diagnóstico y control de la enfermedad.

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Comparison of different enzyme‐linked immunosorbent assay methods for avidity determination of antiphospholipid antibodies

Abstract: This way was in good agreement with more demanding procedures. Both urea and sodium chloride may be used as chaotropic agents. Reference values of avidity indices essential for interpretation of patients' results must be established individually for distinct assay conditions.

Cited by 23 publications

References 31 publications

An Epitope-based Malaria Vaccine Targeting the Junctional Domain of Circumsporozoite Protein

An Epitope-based Malaria Vaccine Targeting the Junctional Domain of Circumsporozoite Protein

Avidity of anti-phospholipid antibodies in relation to their levels

Seroprevalencia de paratuberculosis bovina mediante la prueba de ELISA urea en rodeos de cría y de leche con sospecha de la enfermedad, localizados en la provincia de Buenos Aires, Argentina

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