Comparison of different extraction methods for polysaccharides from Dendrobium officinale stem

He, Lian; Yan, Xiaoteng; Liang, Jian; Li, Shijie; He, Hongrui; Xiong, Qingping; Lai, Xiaoping; Hou, Shaozhen; Huang, Song

doi:10.1016/j.carbpol.2018.06.073

Cited by 172 publications

(95 citation statements)

References 37 publications

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“…Extraction technologies are significantly influential for the utilization of the natural polysaccharide and polyphenolic-protein-polysaccharide complex from medicinal and edible plants, which can affect their extraction yields, structures, and bioactivities [13][14][15]. The conventional hot water extraction (HWE) method is widely applied to extract polysaccharides and polyphenolic-protein-polysaccharide complexes.…”

Section: Introductionmentioning

confidence: 99%

Polyphenolic-Protein-Polysaccharide Complexes from Hovenia dulcis: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro Bioactivities

Xian

et al. 2020

Foods

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Seven extraction methods, including hot water extraction (HWE), pressurized water extraction (PWE), ultrasound-assisted extraction, microwave-assisted extraction, ultrasound-assisted enzymatic extraction, high-speed shearing homogenization extraction, and ultrasound-microwave-assisted extraction, were utilized to extract polyphenolic-protein-polysaccharide complexes (PPPs) from Hovenia dulcis. Next, their physicochemical properties and in vitro antioxidant activities, antiglycation effects, and inhibition activities on α-glucosidase and α-amylase were studied and compared. The findings from this study indicate that various extraction processes exhibit notable influences on the physicochemical properties and in vitro bioactivities of PPPs. Extraction yields, contents of polyphenolics and flavonoids, apparent viscosities, molecular weights, molar ratios of monosaccharide compositions, and ratios of amino acid compositions in PPPs varied in different extraction methods. Furthermore, 13 phenolic compounds in PPPs, including rutin, myricitrin, myricetin, quercetin, kaempferol, protocatechuic acid, gallocatechin, p-hydroxybenzoic acid, ampelopsin, quercetin-7,4′-diglucoside, dihydroquercetin, 5-methylmyricetin, and naringenin, were identified. The relatively strong in vitro antioxidant activities, antiglycation effects, and inhibition activities on α-glucosidase and α-amylase were determined in both PPP-W and PPP-P obtained by HWE and PWE, respectively. The high content of total polyphenolics may be one of the main contributors to their in vitro bioactivities. The findings have shown that the PWE method can be an appropriate method to prepare PPPs with strong bioactivities for application in the functional food industry.

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Section: Introductionmentioning

confidence: 99%

Polyphenolic-Protein-Polysaccharide Complexes from Hovenia dulcis: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro Bioactivities

Xian

et al. 2020

Foods

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“…As an important component of D. officinale, polysaccharide has significant positive effects such as antioxidation, acute colitis, pulmonary fibrosis, and apoptosis (Zhao et al, 2017;Chen et al, 2018a;Liang et al, 2018bLiang et al, , 2019. In order to improve the polysaccharide yield of D. officinale, agricultural and polysaccharide processing approaches such as fertilizer application and optimized polysaccharide extraction and dehydration methods have been applied (He et al, 2018;Qian et al, 2018). However, so far, no satisfactory results have been achieved.…”

Section: Discussionmentioning

confidence: 99%

“…Then the dried stems were grounded to powder with a mortar at room temperature. Around 0.3 g of dry powder was used to extract water-soluble polysaccharides, which were then analyzed by using high-performance liquid chromatography (HPLC) according to the reported method (He et al, 2018). Briefly, the powder of each sample was pre-extracted with 80% ethanol at 80 • C for 2 h. This process was repeated twice to further remove monosaccharides, oligosaccharides, and ethanol-soluble materials.…”

Section: Analysis Of Monosaccharides Composition Of the Polysaccharidesmentioning

confidence: 99%

“…Recently, polysaccharides have been proven to have positive effects on the improvement of antioxidant activity and alleviation of colitis, pulmonary fibrosis, and apoptosis (Huang et al, 2016;Zhang et al, 2017a;Chen et al, 2018a;Liang et al, 2018a). What is more, the bioactivities, composition, structure, and physicochemical properties of polysaccharides from Dendrobium have been well defined (Wei et al, 2016;Zhang et al, 2016;Ma et al, 2018), and a number of critical genes related to polysaccharide synthesis have been characterized as well, such as uridine diphosphate glucose pyrophosphorylases (UGPases), sucrose-phosphate synthases (SPSs), sucrose synthases (SuSys), and glycosyltransferases (GTs) including cellulose synthase like A (CSLAs) genes (He et al, 2015;Yan et al, 2015;Shen et al, 2017). However, systemic study on the polysaccharide biosynthesis pathway is still limited in D. officinale.…”

Section: Introductionmentioning

confidence: 99%

“…Several lines of evidence have demonstrated that the CSLA family plays important roles in the biosynthesis of mannan polysaccharides in many plant species. For example, CSLA genes from guar (Cyamopsis tetragonolobus), Arabidopsis, and poplar (Populus trichocarpa) are capable of producing β-mannan polysaccharides (Dhugga et al, 2004;Liepman et al, 2005;Suzuki et al, 2006;Goubet et al, 2009;He et al, 2015). In higher plants, sucrose metabolism is mainly catalyzed by SPS and SuSy.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Overexpression of DoUGP Enhanced Biomass and Stress Tolerance by Promoting Polysaccharide Accumulation in Dendrobium officinale

et al. 2020

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Uridine diphosphate glucose pyrophosphorylase (UDP-glucose pyrophosphorylase, UGPase), as one of the key enzymes in polysaccharide synthesis, plays important roles in the growth and development of plants. In this study, the DoUGP gene of Dendrobium officinale was overexpressed. The expression of DoUGP and genes playing roles in the same and other saccharide synthesis pathways was determined, and the total soluble polysaccharide was also tested in wild-type and transgenic seedlings. We also performed freezing and osmotic stress treatments to determine whether overexpression of DoUGP could influence stress resistance in transgenic seedlings. Results showed that mRNA expression levels of DoUGP and its metabolic upstream and downstream genes in the transgenic seedlings were increased compared to the expression of these genes in wild-type seedlings. Additionally, most CSLA genes involved in the biosynthesis of mannan polysaccharides were significantly upregulated. The total polysaccharide and mannose content of transgenic seedlings were increased compared to the content of wild type, and enhanced stress tolerance was found in the overexpressed seedlings compared to the wild type.

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Dendrobium officinale polysaccharides—chemical properties and pharmacodynamic effects on the airways in experimental conditions

Šutovská,

Mažerik,

Kocmálová

et al. 2023

Archiv der Pharmazie

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The study aimed to analyze the effects of Dendrobium polysaccharides on the cough and airway reactivity and compare them with the effects of clinically used antitussives (codeine phosphate and butamirate citrate) and bronchodilators (salbutamol), using the guinea pig test system. Dendrobium officinale polysaccharides contained proteins (4.0 wt%) and phenolic compounds (1.7 wt%) with a molecular weight of 25,000 g/mol. The sugar analysis revealed a dominance of glucose (93.7 wt%) and a lesser amount of mannose (5.1 wt%) while other sugar quantities were negligible. Methylation analysis indicated the presence of highly branched polysaccharides. Glucose was found mainly as terminal, 1,4‐ and 1,6‐linked. Furthermore, some 1,4‐ and 1,6‐linked glucose units were found branched at O2, O3, and O6/O4. Mannose was terminal and 1,4‐linked. NMR spectra signals indicate the presence of the (1→4)‐linked α‐d‐glucan, (1→4)‐linked β‐d‐glucan branched at position O6, (1→6)‐linked β‐d‐glucan branched at position O3 and (1→4)‐linked glucomannan. Pharmacological studies showed statistically significant antitussive activity of Dendrobium polysaccharides, exceeding the effect of clinically used antitussives, which may be partially associated with confirmed bronchodilation and the ability of polysaccharides to increase the threshold of cough receptor activation. Dendrobium polysaccharides may increase the possibility of symptomatic treatment of cough, especially in asthmatics.

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Comparison of different extraction methods for polysaccharides from Dendrobium officinale stem

Cited by 172 publications

References 37 publications

Polyphenolic-Protein-Polysaccharide Complexes from Hovenia dulcis: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro Bioactivities

Polyphenolic-Protein-Polysaccharide Complexes from Hovenia dulcis: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro Bioactivities

Overexpression of DoUGP Enhanced Biomass and Stress Tolerance by Promoting Polysaccharide Accumulation in Dendrobium officinale

Dendrobium officinale polysaccharides—chemical properties and pharmacodynamic effects on the airways in experimental conditions

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