Comparison of Different Methods for the Detoxification of Lignocellulose Hydrolyzates of Spruce

Larsson, S; Reimann, Anders; Nilvebrant, Nils‐Olof; Jönsson, Leif J.

doi:10.1007/978-1-4612-1604-9_9

Cited by 41 publications

(65 citation statements)

References 25 publications

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“…In many instances, the most economical and widely used method of detoxification involves treatment of hydrolyzates with solid calcium hydroxide [23]. This process of "overliming" is reported as an effective method of reducing toxicity of various hydrolyzates [24]. However, different yeasts endure the ability of inhibitors differently.…”

Section: Introductionmentioning

confidence: 99%

Adaptation fermentation of Pichia stipitis and combination detoxification on steam exploded lignocellulosic prehydrolyzate

Zhu¹,

Yong²,

Xu³

et al. 2009

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Yeast Pichia stipitis CBS 5776 was developed through adaptation fermentation step by step in steam exploded corn stover prehydrolyzate because high concentration of weak acids and other inhibitors present in the prehydrolyzate could degrade the fermentability. However, the adaptability of Pichia stipitis CBS 5776 in the prehydrolyzate was so limited that steam stripping and overliming were applied to remove these inhibitors from it. Corn stover was steam exploded; the filtrate of steam exploded corn stover was hydrolyzed with dilute sulfuric acid, and then the acid hydrolyzate was detoxified and fermented by Pichia stipitis CBS 5776. Steam stripping could remove volatile compounds from the acid hydrolyzate and the filtrate. At a steam stripping time of 120min, 81% acetic acid and 59% formic acid were removed from the acid hydrolyzate, 77% acetic acid and 45% formic acid were removed from the filtrate, while furfural was stripped off completely from the acid hydrolyzate and the filtrate. Overliming could reduce the contents of furfural and phenolics present in the acid hydrolyzate, however, sugars, especially pentoses, were also removed partially. It was necessary to detoxify the acid hydrolyzate in order to ferment the sugars to ethanol. Acid hydrolyzate detoxified with a combination of steam stripping for 120 min and overliming at pH11 and 60℃ for 90 min, its fermentability was significantly improved. Xylose was consumed nearly completely in 24h with an ethanol yield of 15.92g/l, 80.34% of theoretical.

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Section: Introductionmentioning

confidence: 99%

Adaptation fermentation of Pichia stipitis and combination detoxification on steam exploded lignocellulosic prehydrolyzate

Zhu¹,

Yong²,

Xu³

et al. 2009

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“…The HPX-87 Bio-Rad Ion Exclusive Column (300×7.8 mm, USA) was used for glucose detection, 0.005 M sulfuric acid was supplied at the mobile phase with a flow rate of 0.6 ml/min, and the column temperature was 55 ℃. The concentrations of furfural and 5-HMF were determined with C-18 column (Nucleosil 100-5 C18, Merck, Darmstadt, Germany) with a gradient of 5-100% (v/v) methanol and 0.025% (v/v) of trifluoroacetic acid with a flow rate of 0.8 ml/min, formic acid and acetic acid were determined with AS11HC column which eluted with 80% (v/v) water and 20% (v/v) of a mixture consisting of 0.4 m M Na OH and methanol (50% v/v) at a flow rate of 1.4 m L/min [9].…”

Section: Inhibitor and Sugar Analysis Of Hydrolysatementioning

confidence: 99%

“…As is shown in the following, five strategies have been developed to remove the inhibitors in the acidolysis hydrolysate based on the previous studies with some modifications [9][10][11][12][13]:…”

Section: Detoxificationmentioning

confidence: 99%

“…To date, many of investigations have shown that neutralization, overliming, activated charcoal, ion exchange resin and reducing agents have the ability to remove the inhibitors of the acidolysis hydrolysate [9][10][11][12][13]. In the present study, we employed five different above-mentioned detoxification methods.…”

Section: Fig 3 Inhibitors Concentration Of Raw and Detoxification Hymentioning

confidence: 99%

“…Finally, 6.37 g/L of glucose was achieved based on the following hydrolysis condition: ratio of solid to acid (1:7), acid concentration (32 %), hydrolysis temperature (90 ℃), hydrolysis time (90 min). Since inhibitors (weak acid, furfural, 5-HMF) were formed during acid hydrolysis process [9], we adopted five different detoxification strategies with recombination to remove the inhibition consequence on the fermentation [9][10][11][12][13]. Among five methods, phosphoric acid/calcium hydroxide detoxification combination, the best detoxification way, was applied to remove acetic acid, furfural and 5-HMF about 55.32 %, 99.19 % and 98.22 % respectively.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Isoprenoids Production from Lipid-Extracted Microalgal Biomass Residues Using the Engineered <em>E. coli</em>

Wang¹,

Yang²

2017

Preprint

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Microalgae are recognized as a third generation feedstock for biofuel production due to its rapid growth rate and lignin-free characteristic. In this study, the lipid extracted microalgal biomass residues was used as the material to produce isoprene, α-pinene and β-pinene with the engineered E. coli strain. We adopted an optimal sulfuric acid hydrolysis method (1:7 ratio of solid to acid solution, 32 % (w/v) concentration of sulfuric acid solution at 90℃ for 90 min) to convert holocellulose into glucose efficiently (6.37 g/L) and explored a novel detoxification strategy (phosphoric acid/calcium hydroxide) to remove inhibitors notably. 55.32 % acetic acid, 99.19 % furfural and 98.22 % 5-HMF (5-hydroxymethylfurfural) were cut down with the phosphoric acid/calcium hydroxide method, and the fermentation concentration of isoprene (223.23 mg/L), α-pinene (382.21 μg/L) and β-pinene (17.4 mg/L) using the detoxified hydrolysate as the carbon source account for approximately 86.02 %, 90.16 % and 88.32 % of those produced by the engineered E. coli strain fermented on pure glucose, respectively.

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Fast and efficient alkaline peroxide treatment to enhance the enzymatic digestibility of steam‐exploded softwood substrates

Yang

Boussaid

Mansfield

et al. 2002

Biotech & Bioengineering

145

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The enzymatic digestibility of steam-exploded Douglas-fir wood chips (steam exploded at 195 degrees C, 4.5 min, and 4.5% (w/w) SO(2)) was significantly improved using an optimized alkaline peroxide treatment. Best hydrolysis yields were attained when the steam-exploded material was post-treated with 1% hydrogen peroxide at pH 11.5 and 80 degrees C for 45 min. This alkaline peroxide treatment was applied directly to the water-washed, steam-exploded material eliminating the need for independent alkali treatment with 0.4% NaOH, which has been traditionally used to post-treat wood samples to try to remove residual lignin. Approximately 90% of the lignin in the original wood was solubilized by this novel procedure, leaving a cellulose-rich residue that was completely hydrolyzed within 48 h, using an enzyme loading of 10 FPU/g cellulose. About 82% of the originally available polysaccharide components of the wood could be recovered. The 18% of the carbohydrate that was not recovered was lost primarily to sugar degradation during steam explosion.

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Comparison of Different Methods for the Detoxification of Lignocellulose Hydrolyzates of Spruce

Cited by 41 publications

References 25 publications

Adaptation fermentation of Pichia stipitis and combination detoxification on steam exploded lignocellulosic prehydrolyzate

Adaptation fermentation of Pichia stipitis and combination detoxification on steam exploded lignocellulosic prehydrolyzate

Isoprenoids Production from Lipid-Extracted Microalgal Biomass Residues Using the Engineered <em>E. coli</em>

Fast and efficient alkaline peroxide treatment to enhance the enzymatic digestibility of steam‐exploded softwood substrates

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