Comparison of different sorbent materials for solid-phase extraction of selected drugs in human urine analyzed by UHPLC–UV

Magiera, Sylwia; Hejniak, Judyta; Baranowski, J. M.

doi:10.1016/j.jchromb.2014.03.014

Cited by 22 publications

(5 citation statements)

References 38 publications

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“…The 1 st layer of filtering consists of removing salts, urea and macromolecular aggregates from urine and changing the solvent to maximize the catalyst performance, all of which are achieved by Solid Phase Extraction (SPE). Screening of a variety of commercially available SPE cartridges, differing by their selectivity and the retention mechanism, 17 and optimization of the extraction protocol was achieved by targeting endogenous nicotinamide (NA). This compound occurs in urine at low-μM concentrations 6 and can be detected by NMR using SABRE.…”

Section: Resultsmentioning

confidence: 99%

NMR detection in biofluid extracts at sub-μM concentrations via para-H₂ induced hyperpolarization

Reile¹,

Eshuis²,

Hermkens³

et al. 2016

Analyst

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NMR spectroscopy is one of the most powerful techniques to simultaneously obtain qualitative and quantitative information in chemical analysis. Despite its versatility, the applications of NMR in the study of biofluids are often limited by the insensitivity of the technique, further aggravated by the poor signal dispersion in the (1)H spectra. Recent advances in para-H2 induced hyperpolarization have proven to address both these limitations for specific classes of compounds. Herein, this approach is for the first time applied for quantitative determination in biofluid extracts. We demonstrate that a combination of solid phase extraction, para-hydrogen induced hyperpolarization and selective NMR detection quickly reveals a doping substance, nikethamide, at sub-μM concentrations in urine. We suggest that this method can be further optimized for the detection of different analytes in various biofluids, anticipating a wider application of hyperpolarized NMR in metabolomics and pharmacokinetics studies in the near future.

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Section: Resultsmentioning

confidence: 99%

NMR detection in biofluid extracts at sub-μM concentrations via para-H₂ induced hyperpolarization

Reile¹,

Eshuis²,

Hermkens³

et al. 2016

Analyst

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“…The proposed method is simple, and unlike conventional SPE method, it does not require a lengthy evaporation step. [51][52][53] The tested drugs demonstrated good recoveries when spiked to urine samples (72.8-116%). Another strength of the work lies in the fact that the high potential of the extracting the compounds with diverse polarities simultaneously.…”

Section: Comparison With Previously Reported Analytical Methodsmentioning

confidence: 95%

Graphene and zeolite as adsorbents in bar-micro-solid phase extraction of pharmaceutical compounds of diverse polarities

et al. 2021

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“…If the template molecule is the smallest one in the group, the other molecules are not expected to fit into the cavities created and then the specificity is achieved. In addition to their selectivity and specificity, MIPs are preferred due to their low cost, ease of preparation in a short time, having high stability and activity during a wide range of conditions and robustness [ 7 , 13 , 14 ]. The SPE method in which MIPs are used as sorbents is called molecular imprinted solid-phase extraction (MISPE) [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

A molecularly imprinted polymer as solid phase extraction sorbent for ketoprofen determination in water and artificial serum prior to HPLC

ÖZYURT¹,

ÖZYURT²,

ALTINSOY³

et al. 2022

Turkish Journal of Chemistry

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Ketoprofen (KET) is an active pharmaceutical compound that has pain relieving and antipyretic effects. Its determination in body fluids and environmental waters is important due to widespread use of the compound. In this study, a selective and reliable method has been developed for the determination of ketoprofen in water and artificial serum using molecularly imprinted polymers (MIPs) as a solid phase extraction sorbent prior to HPLC-DAD detection. The MIP was synthesized by copolymerization of methacrylic acid (MAA) and trimethylpropane trimethacrylate (TRIM) in the presence of ketoprofen as the template. For the sake of comparison, nonimprinted polymer (NIP) was also synthesized under the same experimental conditions without the addition of ketoprofen under the same experimental conditions. Critical extraction parameters such as sample pH, shaking time and sorbent amount were optimized and adjusted to 8.0, 24 h, and 10.0 mg, respectively, for a sample volume of 10.0 mL. MIP showed higher selectivity than NIP towards ketoprofen in an artificial matrix containing another pain relieving drug, ibuprofen, and a cardiovascular drug, metoprolol. The proposed method was successfully applied for the detection of ketoprofen in spiked drinking water, tap water, and artificial serum samples, and showed satisfactory results with respective recoveries of 96.8 % (± 0.8), 93.7% (± 0.6), 62.2% (± 0.6), and 69.9% (± 0.6).

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Comparison of different sorbent materials for solid-phase extraction of selected drugs in human urine analyzed by UHPLC–UV

Cited by 22 publications

References 38 publications

NMR detection in biofluid extracts at sub-μM concentrations via para-H₂ induced hyperpolarization

NMR detection in biofluid extracts at sub-μM concentrations via para-H₂ induced hyperpolarization

Graphene and zeolite as adsorbents in bar-micro-solid phase extraction of pharmaceutical compounds of diverse polarities

A molecularly imprinted polymer as solid phase extraction sorbent for ketoprofen determination in water and artificial serum prior to HPLC

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Comparison of different sorbent materials for solid-phase extraction of selected drugs in human urine analyzed by UHPLC–UV

Cited by 22 publications

References 38 publications

NMR detection in biofluid extracts at sub-μM concentrations via para-H2 induced hyperpolarization

NMR detection in biofluid extracts at sub-μM concentrations via para-H2 induced hyperpolarization

Graphene and zeolite as adsorbents in bar-micro-solid phase extraction of pharmaceutical compounds of diverse polarities

A molecularly imprinted polymer as solid phase extraction sorbent for ketoprofen determination in water and artificial serum prior to HPLC

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NMR detection in biofluid extracts at sub-μM concentrations via para-H₂ induced hyperpolarization

NMR detection in biofluid extracts at sub-μM concentrations via para-H₂ induced hyperpolarization