Comparison of Etest with Modified Broth Microdilution Method for Testing Susceptibility of
            <i>Aspergillus</i>
            spp. to Voriconazole

Serrano, M. C. López; Morilla, D.; Valverde, Anastasio; Diego, María José Chávez de; Espinel-Ingroff, Ana; Claro, R.; Ramírez, M.; Martín-Mazuelos, Estrella

doi:10.1128/jcm.41.11.5270-5272.2003

Cited by 24 publications

(19 citation statements)

References 27 publications

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“…In the reference microdilution method, all the strains were inhibited by Յ0.5 mg of voriconazole/liter; similar results were found by other authors (5,6,8,15,18). Practically no differences were observed among both microdilution methods; by the Sensititre method, all the strains were inhibited by 1 mg of drug/liter.…”

supporting

confidence: 77%

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Comparison of the Sensititre YeastOne Colorimetric Antifungal Panel with a Modified NCCLS M38-A Method To Determine the Activity of Voriconazole against Clinical Isolates of Aspergillus spp

et al. 2004

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The susceptibilities of 63 isolates of Aspergillus spp. to voriconazole were evaluated by a modified NCCLS M38-A method and the Sensititre YeastOne method. The overall agreement was 82.5%, ranging from 100% for Aspergillus niger and Aspergillus terreus to 62.5% for Aspergillus flavus. Discrepancies between the methods were due to higher Sensititre MICs. The Sensititre YeastOne method could have potential value for susceptibility testing of Aspergillus spp. to voriconazole.Over the past decades, the incidence of invasive fungal infections has increased, especially those caused by Aspergillus species (18). The treatment of choice for infected patients remains amphotericin B, although alternative drugs with activities against Aspergillus species are becoming available for clinical use, including antifungal azoles and echinocandins (6,18). Because the number of serious infections caused by Aspergillus spp. has increased, as has the resistance of Aspergillus spp. to established agents, determination of the in vitro susceptibilities of Aspergillus isolates to both established and investigational agents is necessary (6).There is a great need for an easier and reproducible method for in vitro susceptibility testing of filamentous fungi as a guide to selecting and monitoring antifungal therapy. Alternatives to the NCCLS method are currently under investigation, including two commercial methods, E-test and Sensititre YeastOne, which have been evaluated for yeast and molds (1,3,5,6,9,10,11,(13)(14)(15)(16)(17).Our study represents an evaluation of the suitability of Sensititre YeastOne for determining the susceptibilities of Aspergillus spp. isolates to voriconazole. This test is a commercial colorimetric panel that consists of a disposable tray which contains dried serial dilutions of five antifungal agents in individual wells. The wells also contain an oxidation-reduction indicator (Alamar Blue) to generate clear-cut endpoints based on a visually detectable color change. MICs obtained by this method were compared to those obtained by the modified reference broth microdilution method (14).A total of 63 clinical Aspergillus isolates, comprising 24 Aspergillus fumigatus isolates, 16 Aspergillus flavus isolates, 9 Aspergillus terreus isolates, 8 Aspergillus niger isolates, 4 Aspergillus glaucus isolates, and 2 Aspergillus nidulans isolates, were included in this study. These isolates were recovered from clinical specimens of patients at the Valme University Hospital, Seville, Spain, and the Division of Infectious Diseases, Medical College of Virginia, Virginia Commonwealth University, Richmond.Culture, identification, and preservation of strains were done by using routine mycological methods (4, 7). Candida krusei ATCC 6258 and Candida parapsilosis ATCC 22019 were used as quality control strains for susceptibility testing procedures, and Aspergillus fumigatus ATCC 204305 and Aspergillus flavus ATCC 204304 were used as reference strains.The broth microdilution method was performed according to modified NCCLS standard M38-A gu...

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supporting

confidence: 77%

“…Alternatives to the NCCLS method are currently under investigation, including two commercial methods, E-test and Sensititre YeastOne, which have been evaluated for yeast and molds (1,3,5,6,9,10,11,(13)(14)(15)(16)(17).…”

mentioning

confidence: 99%

Comparison of the Sensititre YeastOne Colorimetric Antifungal Panel with a Modified NCCLS M38-A Method To Determine the Activity of Voriconazole against Clinical Isolates of Aspergillus spp

et al. 2004

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“…So, these results demonstrate an apparent increased sensitivity of the agar testing method over the microdilution assay. In the majority of studies, the agreement between the MICs of these 2 methods varies since these test systems measure different interactions between an antimicrobial agent and a microorganism (Mayrhofer et al, 2008;Serrano et al, 2003;Szekely et al, 1999;van der Heijden et al, 2007). Therefore, it is expected that there will be some minor differences between the systems (Rennie et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the best method to assess antibiotic potentiation by phytochemicals against Staphylococcus aureus

Abreu

Serra

Borges

et al. 2014

Diagnostic Microbiology and Infectious Disease

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The increasing occurrence of bacterial resistance to antibiotics has now reached a critical level. Finding antibiotic coadjuvants capable to inhibit the bacterial resistance mechanisms would be a valuable mid-term solution, until new classes of antibiotics are discovered. Selected plant alkaloids were combined with 5 antibiotics against 10 Staphylococcus aureus strains, including strains expressing distinct efflux pumps and methicillin-resistant S. aureus strains. The efficacy of each combination was assessed using the microdilution checkerboard, time-kill, Etest, and disc diffusion methods. The cytotoxicity of the alkaloids was evaluated in a mouse fibroblast cell line. Potentiation was obtained in 6% of all 190 combinations, especially with the combination of: ciprofloxacin with reserpine (RES), pyrrolidine (PYR), and quinine (QUIN); tetracycline with RES; and erythromycin with PYR. The highest cytotoxicity values were found for QUIN (half maximal inhibitory concentration [IC50] = 25 ± 2.2 mg/L) and theophylline (IC50 = 100 ± 4.7 mg/L).

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“…Although NCCLS suggests that broth dilution tests should be employed for filamentous fungi (standard M-38A), agar-based methods such as E-test and disc diffusion are good alternatives to broth dilution testing, once they are simpler, faster and show good correlation with broth microdilution methods. 15,16 Accordingly, it was possible to establish the antifungal susceptibility profile of 33 imidazole and triazole derivatives against M. perniciosa. Our biological assay shows that azole derivatives depicted in Figure 1 do not inhibit fungal growth under assayed conditions.…”

Section: Resultsmentioning

confidence: 99%

In vitro screening and chemometrics analysis on a series of azole derivatives with fungicide activity against moniliophthora perniciosa

Mota¹,

Barros²,

Castilho³

2010

J. Braz. Chem. Soc.

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Moniliophthora perniciosa, o agente causal da vassoura-de-bruxa do cacaueiro, diminuiu significativamente a produção de cacau, especialmente no estado da Bahia, a maior região produtora de cacau do continente americano. As formas de controle desenvolvidas até o momento têm baixa eficiência. Derivados de azol são ativos tanto in vitro quanto in loco contra M. perniciosa, porém não há um estudo sistemático sobre a atividade dos azóis contra este fitopatogeno. Dados biológicos, obtidos em um ensaio padronizado, foram utilizados para criação de modelos quimiométricos, que destacam características físico e estruturais importantes para a atividade fungicida de derivados de azol frente a M. perniciosa. De acordo com os modelos de PCA e SIMCA, características eletrônicas, representadas pelos descritores BEHe3 e JGI4, paralelamente à possibilidadde de realizar ligações de H e ausência de átomos de cloro, distantes de 6-8 ligações dos nitrogênios do anel azólico, parecem contribuir para atividade fungicida dos compostos estudados Moniliophthora perniciosa, the causal agent of witches' broom disease in Theobroma cacao, significantly decreased cacao production, especially in Bahia State, the largest cocoa producing of the American continent. Control programs developed so far have low efficiency. Azole derivatives are active both in vitro and in loco against M. perniciosa, however there is no comprehensive study on the activity of azoles against this phytopatogen. Standardized in vitro biological data were employed to develop supervised and unsupervised chemometric models that highlight physicochemical and structural features that are crucial for azole's fungicidal activity against M. perniciosa. Thus, PCA and SIMCA models suggest that electronegativity (BEHe3) and dipolar moment (JGI4), as well as H-bonding to M. pernciosa's lanosterol 14α-desmethylase active site and lack of Cl atoms 6 to 8 bonds from the azole's nitrogen atoms play a major role to azoles' fungicide activity.

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Comparison of Etest with Modified Broth Microdilution Method for Testing Susceptibility of Aspergillus spp. to Voriconazole

Cited by 24 publications

References 27 publications

Comparison of the Sensititre YeastOne Colorimetric Antifungal Panel with a Modified NCCLS M38-A Method To Determine the Activity of Voriconazole against Clinical Isolates of Aspergillus spp

Comparison of the Sensititre YeastOne Colorimetric Antifungal Panel with a Modified NCCLS M38-A Method To Determine the Activity of Voriconazole against Clinical Isolates of Aspergillus spp

Evaluation of the best method to assess antibiotic potentiation by phytochemicals against Staphylococcus aureus

In vitro screening and chemometrics analysis on a series of azole derivatives with fungicide activity against moniliophthora perniciosa

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