Comparison of extraction sequence on yield and physico-chemical characteristic of chitosan from shrimp shell waste

Abstract

The focus of this study was to compare the yield and characteristics of chitosan produced from different sequences of treatment for deproteination, demineralization and deacetylation. In Process 1, deproteination occurred in the first step followed by demineralization and deacetylation. While Process 2 was started with demineralization step and followed by deproteination and deacetylation. The results show that the percentage yield of chitosan was slightly higher when Process 2 was adopted with 22.22 %. However, the process produced chitosan with lower degree of deacetylation and solubility with high ash content, which were 76.47 %, 37.79 % and 2.67 %, respectively. While, when Process 1 was carried out, the degree of deacetylation of chitosan, solubility and ash content were improved to 91.26 %, 100 % and 0.38 %, respectively, with lower yield of 19.01 %. Therefore, this study suggests the extraction process should be performed by carrying out deproteination step before demineralization and deacetylation (Process 1) to produce a good quality of chitosan.

Biodegradable plastic formulated from chitosan of Aristeus antennatus shells with castor oil as a plasticizer agent and starch as a filling substrate

“…It almost exactly matches William and Wid's 35 findings, who found that the average amount of chitosan produced from the wet weight of shrimp shell waste was 19.01%. Because the natural structure of chitin (a protein) was lost during the deproteination process, less chitosan was generated when extraction was started with deproteinization.…”

“…The ratio of the dry weight of chitosan flakes to the wet weight of shrimp shell, as determined by Eq. ( 1 ), was used to calculate the chitosan yield (%) 35 : …”

Response Surface Methodology (RSM) Approach to Optimization of Coagulation-Flocculation of Aquaculture Wastewater Treatment Using Chitosan from Carapace of Giant Freshwater Prawn Macrobrachium rosenbergii

“…This study observed that 60% NaOH applied in the deacetylation of chitin resulted in a solubility and DDA of 71.23% and 85.20%, respectively ( Table 4 ). The solubility and DDA of chitosan in this study would have been higher than the values obtained but for the relatively high ash content [ 64 ]. It is on record that the solubility and DDA of chitosan obtained from insect cuticle [ 18 ], deep sea mud [ 54 ], and fungi [ 65 ] are all lower than the results of the present study.…”

Pacific white shrimp ( Litopenaeus vannamei ) shell chitosan and the conjugate with epigallocatechin gallate: Antioxidative and antimicrobial activities

“…Due to intermolecular and intramolecular water traces, O–H and N–H peaks might overlap with each other to form a wide band that appeared in the same area for both groups (Figure 1). The C–H stretching, both symmetric and asymmetric, was observed at 2,921 to 2,875 cm −1 (William & Wid, 2019). For amide‐I, representing CO stretching from the acetyl group, it was observed at 1,650 cm −1 (Trung & Bao, 2015).…”