Comparison of Flow Cytometric Dna Content in the Primary Tumor and in the Corresponding Lymph-Node Metastasis of Patients With Squamous Cells Carcinomas of the Head and Neck

Hemmer, Joerg; Krause, Hr; Thein, Thomas; E, Schön; Kreidler, J

doi:10.3892/ijo.1.5.551

Cited by 6 publications

(6 citation statements)

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“…Aneuploid tumor cells have a pronounced potential of invasive growth and metastatic spread. In support of previous results,2, 11 about half of the aneuploid carcinomas in this study were associated with lymph node metastasis at presentation. Another one‐fourth of the aneuploid lymph node negative group presented with involved lymph nodes at second surgery.…”

Section: Discussionsupporting

confidence: 93%

“…A high degree of concordance between the DNA profiles of primary tumors and those of their corresponding recurrences indicated a definite cytogenetic stability of initially established aneuploid clones, in spite of a great variability of individual DNA contents 1. This observation has also been made with respect to synchronous lymph node metastases 11. This was in spite of changes in the DNA contents of approximately one of three cases, and may reflect a selection of aneuploid subclones with secondary cytogenetic alterations that have made advantageous phenotypic adaptations to the therapeutically modified microenvironment.…”

Section: Discussionmentioning

confidence: 70%

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The value of DNA flow cytometry in predicting the development of lymph node metastasis and survival in patients with locally recurrent oral squamous cell carcinoma

Hemmer

Thein

Heerden

1997

Cancer

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conducted to examine the importance of DNA ploidy in patients with locally recurrent oral carcinoma. RESULTS.Eight patients with diploidy of both the primary tumor and the recurMilitary Hospital Ulm, Ulm, Germany.rence never developed lymph node metastasis. The 5-year overall survival rate of 3 Department of Oral Pathology and Oral Biolthis group was 87%. For 80 aneuploid primary carcinomas, recurrences developed ogy, University of Pretoria, Pretoria, Republic that were also aneuploid. Only 31% of these patients were 5-year survivors (P õ of South Africa.0.001). Lymph node metastasis at presentation was found in 55% of this group, whereas 13% of initially lymph node negative patients presented with regional disease at second surgery. Five of 13 diploid primary tumors recurred with aneuploid cell lines. Three of these five patients died, two with regional metastasis. The 5-year survival rate of patients with aneuploid recurrences who were never afflicted with lymph node involvement (41%) was better (P õ 0.05) than the 5-year survival rate of those with metastasis at presentation or at second surgery (26%). D NA flow cytometry studies provide substantial evidence that the development of aneuploid tumor cell populations from diploid progenitor cells contribute significantly to the behavior of oral carcinoma. 1,2 With radical surgery, a 5-year survival rate of nearly 90% was Supported by a grant from Rudolf und Clothilde achieved for patients with diploid primary squamous cell carcinoma, Eberhardt-Stiftung, Ulm, Germany. in contrast to a rate of approximately 30% for patients with aneuploid primary tumors. This suggests that prognosis can be improved by been reported to range between 46% and 71% for surgically resected aneuploid oral carcinomas, compared with between 4% and 9% for CONCLUSIONS.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 70%

The value of DNA flow cytometry in predicting the development of lymph node metastasis and survival in patients with locally recurrent oral squamous cell carcinoma

Hemmer

Thein

Heerden

1997

Cancer

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“…It seems reasonable, however, to assume a correlation between primary tumour and metastasis since there seems to be a good concordance between FCM DNA content in primary and metastatic HNSCC. Hemmer and coworkers [12] studied 48 patients with HNSCC. Three primary tumours were diploid, two associated with diploid metastases and one with an aneuploid metastasis.…”

Section: Discussionmentioning

confidence: 99%

Distribution of Non-Diploid Flow-Cytometric DNA Indices and Their Relation to the Nodal Metastasis in Squamous Cell Carcinomas of the Head and Neck

Wennerberg

Baldetorp²,

Wahlberg³

1998

Invasion Metastasis

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Squamous cell carcinomas of the head and neck (HNSCC) evolve from diploid epithelial cells of the mucosa. At the time of diagnosis about two thirds of clinically diagnosed HNSCC are non-diploid according to flow-cytometric (FCM) analysis, indicating that during tumour progression there must be an acquisition and accumulation of chromosomal aberrations. At diagnosis one third to one half of HNSCC have clinically positive neck nodes. The objective of the present study was to see whether the progression to a metastatic phenotype is reflected in the distribution of FCM DNA ploidy in node-negative and node-positive HNSCC. The series comprised 200 patients with HNSCC. Tumour samples were obtained from diagnostic biopsies or primary surgery. A multistep preparation method and propidium iodide staining of nuclear DNA content was used for FCM. One hundred and forty one (71%) of the tumours were non-diploid. Only two tumours were hypodiploid (DNA index 0.73 and 0.93, respectively). Ten of the tumours exhibited two non-diploid stem cell lines. The frequency of non-diploidy in node-negative tumours was 65% and in node-positive ones about 80%. The frequency distribution of non-diploid DNA indices clustered in the hypotetraploid region (with a modal value of 1.71–1.74) and did not differ between node-negative and node-positive tumours. The hypothesis that the disposition to metastasis is reflected in the frequency distribution of non-diploid DNA indices could thus not be verified.

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“…That tumor progression can be interrupted in diploid oral carcinomas even by surgery alone, but failed to be curative in the majority of aneuploid cases treated similarly, has re cently been reported [4], Lymph node in volvement nearly exclusively in patients with aneuploid primary tumors [6][7][8] represents a further indication that aneuploid oral carci noma cells, unlike diploid tumor cells, indeed share the property of an increased biological malignancy in terms of an enhanced potential of invasion and metastasis, probably acti vated by the cytogenetic events associated with aneuploidy formation.…”

Section: Discussionmentioning

confidence: 99%

“…The 5-year overall survival rate in patients with diploid carcinomas was 88%, in contrast to only 36% in the aneuploid group. Furthermore, cervical lymph node involve ment has only occasionally been seen in oral carcinoma patients with primary tumors con sisting exclusively of diploid cells, while more than 90% of all patients with lymph node metastases at presentation belonged to the aneuploid group [6][7][8],…”

Section: Introductionmentioning

confidence: 99%

Anticancer Drug Action Assessed by Serial DNA Flow Cytometry during Induction Chemotherapy in Oral Squamous Cell Carcinoma: Impacts on the Development of Individualized Treatment Strategies

Hemmer

1995

Chemotherapy

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Biopsy specimens from 53 advanced oral squamous cell carcinomas were taken before and after intra-arterial induction chemotherapy and analyzed by DNA flow cytometry. The 5-year overall survival rate was 90% in patients with diploid carcinomas in contrast to only 18% of those with pretherapeutically aneuploid tumors. A clear decrease of aneuploid cell numbers during chemotherapy indicated that DNA flow cytometry substantially allows monitoring of cytotoxic effects in vivo. Even if the immediate response to chemotherapy was significantly better in carcinomas with a complete disappearance of aneuploid tumor cells during treatment than in cases with persisting aberrant cells, the overall survival rate was nearly identical in both groups.

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Comparison of Flow Cytometric Dna Content in the Primary Tumor and in the Corresponding Lymph-Node Metastasis of Patients With Squamous Cells Carcinomas of the Head and Neck

Cited by 6 publications

References 0 publications

The value of DNA flow cytometry in predicting the development of lymph node metastasis and survival in patients with locally recurrent oral squamous cell carcinoma

The value of DNA flow cytometry in predicting the development of lymph node metastasis and survival in patients with locally recurrent oral squamous cell carcinoma

Distribution of Non-Diploid Flow-Cytometric DNA Indices and Their Relation to the Nodal Metastasis in Squamous Cell Carcinomas of the Head and Neck

Anticancer Drug Action Assessed by Serial DNA Flow Cytometry during Induction Chemotherapy in Oral Squamous Cell Carcinoma: Impacts on the Development of Individualized Treatment Strategies

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