Comparison of four methods for the determination of plasma pepsinogen concentration

Scott, Ian; Stear, M. J.; McKellar, Quintin

doi:10.1016/0034-5288(95)90009-8

Cited by 13 publications

(2 citation statements)

References 11 publications

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“…Synthetic human nsG17 (Research Plus, USA) was used to prepare radiolabel and standards. Pepsinogen concentrations were determined by a method validated by Scott et al [ 58 ], with minor modifications [ 59 ]. Peptic activity was calculated from the tyrosine-containing peptide fragments liberated by digestion of glycine-buffered BSA, following the conversion of pepsinogen to pepsin at pH< 2.…”

Section: Methodsmentioning

confidence: 99%

Abomasal dysfunction and cellular and mucin changes during infection of sheep with larval or adult Teladorsagia circumcincta

et al. 2017

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This is the first integrated study of the effects on gastric secretion, inflammation and fundic mucins after infection with L3 T. circumcincta and in the very early period following transplantation of adult worms. At 3 months-of-age, 20 Coopworth lambs were infected intraruminally with 35,000 L3; infected animals were killed on Days 5, 10, 15, 20 and 30 post-infection and 6 controls on either Day 0 or 30 post-infection. Another 15 Romney cross lambs received 10,000 adult worms at 4–5 months-of-age though surgically-implanted abomasal cannulae and were killed after 6, 12, 24 and 72 hours; uninfected controls were also killed at 72 hours. Blood was collected at regular intervals from all animals for measurement of serum gastrin and pepsinogen and abomasal fluid for pH measurement from cannulated sheep. Tissues collected at necropsy were fixed in Bouin’s fluid for light microscopy, immunocytochemistry and mucin staining and in Karnovsky's fluid for electron microscopy. Nodules around glands containing developing larvae were seen on Day 5 p.i., but generalised effects on secretion occurred only after parasite emergence and within hours after transplantation of adult worms. After L3 infection, there were maximum worm burdens on Days 10–15 post-infection, together with peak tissue eosinophilia, inhibition of gastric acid secretion, hypergastrinaemia, hyperpepsinogenaemia, loss of parietal cells, enlarged gastric pits containing less mucin and increased numbers of mucous neck cells. After adult transplantation, serum pepsinogen was significantly increased after 9 hours and serum gastrin after 18 hours. Parallel changes in host tissues and the numbers of parasites in the abomasal lumen suggest that luminal parasites, but not those in the tissues, are key drivers of the pathophysiology and inflammatory response in animals exposed to parasites for the first time. These results are consistent with initiation of the host response by parasite chemicals diffusing across the surface epithelium, possibly aided by components of ES products which increased permeability. Parietal cells appear to be a key target, resulting in secondary increases in serum gastrin, pit elongation, loss of surface mucins and inhibition of chief cell maturation. Inflammation occurs in parallel, and could either cause the pathology or exacerbate the direct effects of ES products.

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Section: Methodsmentioning

confidence: 99%

Abomasal dysfunction and cellular and mucin changes during infection of sheep with larval or adult Teladorsagia circumcincta

et al. 2017

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“…Sodium and potassium were estimated by using flame photometer and chloride by using chloride meter. Colourometric methods were used for determination of pepsinogen (Scott et al, 1995) and α-tocopherol (Quaife et al, 1949).…”

Section: Serum Biochemical Analysismentioning

confidence: 99%

A Preliminary Study on Cryptosporidiosis in Dromedary Camels at Shalatin Area, Egypt

2007

Assiut Veterinary Medical Journal

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Pepsinogens: Physiology, Pharmacology Pathophysiology and Exercise

Gritti

Banfi

Roi

2000

Pharmacological Research

120

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Comparison of four methods for the determination of plasma pepsinogen concentration

Cited by 13 publications

References 11 publications

Abomasal dysfunction and cellular and mucin changes during infection of sheep with larval or adult Teladorsagia circumcincta

Abomasal dysfunction and cellular and mucin changes during infection of sheep with larval or adult Teladorsagia circumcincta

A Preliminary Study on Cryptosporidiosis in Dromedary Camels at Shalatin Area, Egypt

Pepsinogens: Physiology, Pharmacology Pathophysiology and Exercise

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