Comparison of four synchronization protocols for fixed-time bovine embryo transfer in Bos indicus × Bos taurus recipients

Nasser, L.F.; Reis, E. L; Oliveira, Marcos Antônio Lemos; Bó, G. A.; Baruselli, Pietro Sampaio

doi:10.1016/j.theriogenology.2004.03.013

Cited by 38 publications

(32 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This result is less than those found in similar studies concerning cattle Bos taurus x B. indicus, wherein the percentages of use of the protocol ranged from 82% to 93% (Nasser et al 2004, Looney et al 2006. Initially, this finding may have resulted from the cow synchronization without CL, and/or of the negative energy balance; facts directly related to a delay in ovulation (Beam and Butler 1998).…”

Section: Discussioncontrasting

confidence: 61%

See 1 more Smart Citation

Immune-serological identification of infectious agents with influence on bovine embryo transfer in the north of Brazil

et al. 2016

View full text Add to dashboard Cite

ABSTRACT. The aim of this study was to investigate antibodies against brucellosis, leptospirosis, bovine rhinotracheitis (IBR/ BoHV-1), bovine viral diarrhea (BVD) and neosporosis through immunodiagnostic. These agents may directly or indirectly compromise the female bovine reproductive tract as well as embryonic and fetal development, therefore we also tried to establish a probable relation between those antibody with the utilization rates of fixed-time embryo transfer (FTET) protocols, with pregnancy rate per embryo transfer (ET) and the occurrence of miscarriages. Embryos were obtained by in vitro fertilization of the ovum and sperm from the Gyr dairy breed, which were transferred to 235 zebu-crossbred cows under FTET protocols. The blood samples to obtain serum were collected by venous puncture from coccygeal vein, only a few days of the completion of ET. For the immunodiagnostic research of brucellosis, leptospirosis, IBR/BoHV-1, BVD and neosporosis, the complement fixation assay, the microscopic agglutination test, virus neutralization, and linked immunosorbent assay to enzymes in conjunction with the indirect immunofluorescence were used. The utilization rates of the FTET protocols related to pregnancy and abortion in D16, D35, and D75 were 67.23%, 34,18% and 20,37% respectively. The immunodiagnostic results related to seropositive animals were obtained for brucellosis (7/235 = 2.89%), leptospirosis (128/235 = 54.47%), IBR (103/235 = 43.83%), BVD (174/235 = 74.04%), and neosporosis (150/235 = 63.82%). In seropositive recipients, there was an observed influence (P < 0.05) of leptospirosis and neosporosis on the rate of protocol utilization of brucellosis on both the pregnancy and abortion rate, and of BVD and IBR on abortion rate.Key words: reproductive, embryo, recipients, cattle. RESUMEN.El estudio tuvo por finalidad evidenciar, mediante técnicas de inmunodiagnóstico, anticuerpos contra Brucelosis, Leptospirosis, Rinotraqueítis Infecciosa Bovina (IBR), Diarrea Viral Bovina (DVB) y Neosporosis, agentes que directa o indirectamente pueden comprometer el tracto reproductor de las hembras así como el desarrollo embrionario y fetal. Se estableció una probable relación entre la presencia de anticuerpos contra los agentes con la tasa de aprovechamiento de protocolos de transferencias de embriones en tiempo fijo (TETF), la tasa de preñez por transferencia de embriones (TE) y la ocurrencia de abortos. Los embriones fueron obtenidos por fertilización in vitro (FIV) a partir de aspiración folicular y semen de toros, ambos de raza Gyr lechero, y tranferidos a 235 vacas receptoras cruzadas con Cebú sometidas a protocolos para TETF. Se obtuvo suero de muestras de sangre mediante venopunción de la vena coccígea solamente en los días de realización de TE. Para evidenciar Brucelosis, Leptospirosis, IBR, DVB y Neospora se emplearon las técnicas de fijación de complemento, seroaglutinación microscópica, seroneutralización y test de inmunoensayo (ELISA) junto con la inmunofluorescencia indirecta (RIFI), respectivamente. El ...

show abstract

Section: Discussioncontrasting

confidence: 61%

“…This utilization rate of protocol was lower than expected, as reported in other studies (Nasser et al 2004, Looney et al 2006. In addition, the above is related to the action mechanism of the agent (Neill 2013).…”

Section: Discussioncontrasting

confidence: 46%

Immune-serological identification of infectious agents with influence on bovine embryo transfer in the north of Brazil

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Cows of G1 and G2 showed estrus in the 55.5th and 56.8th day, respectively, versus in the 84,5th (G3). These results are confirmed by Nasser et al (2004), Bo et al (2007), Bryan et al (2008) and Souza (2008), who registered the advantages of using similar protocols in FTAI in the intgerval calving-conception.…”

Section: Resultssupporting

confidence: 52%

Reduction of the Voluntary Waiting Period by Use of Hormonal Protocols and Fixed-Time Artificial Insemination (Ftai) in Dairy Cows

Suplicy¹,

Filho²,

Kozicki³

et al. 2012

AVS

View full text Add to dashboard Cite

ABSTRACT:The aim of this study was to reduce the interval calving-conception in dairy cows by administration of two different hormonal protocols for estrus synchronization and fixed-time artificial insemination (FTAI). Holstein dairy cows (n=128) were divided in three groups (G): G1 (44 treated), G2 (34 treated) and G3 (50 as control). In the treated cows the following hormonal protocols were applied between 40 and 45 days post partum: G1-Day 0 (D0) -bovine intravaginal device containing progesterone (P 4 ) plus 1.0 mg of estradiol benzoate (EB), D8 -removal of the P 4 , plus 0.5 mg of cloprostenol plus 400 IU of equine chorionic gonadotropin (eCG), D9 -1.0 mg of EB and FTAI after 36 hours. G2 -D0 -P 4 intravaginal device plus 50 mcg of gonadotropin-releasing hormone (GnRH), D7 -removal of the P 4 plus cloprostenol plus 400 IU eCG; D9 -50 mcg of GnRH and FTAI 16 hours after. G3 -Control group inseminated 12 hours after the visual estrus. Results: G1 and G2 were inseminated (FTAI) respectively in the D55.5±0.9 and D56.8±1.3 after calving, while the G3 in the D84.5±5.7 (p<0.05). The pregnancy rate in the G1, G2 and G3 was respectively 47.0, 46.1 and 35.8% (p>0.09). In conclusion: there was significant reduction of the interval calving/conception in treated cows (G1,G2)(p<0.05) and better pregnancy rate regarding to G3 Key Words: interval post partum period; pregnancy rate; dairy cows; progesterone; time artificial insemination REDUÇÃO DO INTERVALO PARTO-CONCEPÇÃO MEDIANTE O EMPREGO DE PROTOCOLOS HORMONAIS VISANDO A INSEMINAÇÃO ARTIFICIAL EM TEMPO FIXO (IATF) EM VACAS LEITEIRASRESUMO: O objetivo deste estudo foi reduzir o intervalo parto -concepção em vacas leiteiras, mediante a administração de dois diferentes protocolos hormonais para a sincronização do estro e inseminação artificial em tempo fixo (IATF). Vacas leiteiras da raça Holandesa (n=128) foram divididas em três grupos (G): G1 (n=44); G2 (n=34) e G3 (n=50). Nos grupos tratados G1 e G2 administrou-se entre o 40º e o 45º dia pós parto: G1-Dia 0 (D0) -dispositivo intravaginal bovino contendo progesterona (P 4 ) mais 1,0 mg de benzoato de estradiol (BE-IM), D8 -remoção da P 4 mais 0,5 mg de prostaglandina F2 alfa (PGF2α) mais 400 UI de gonadotrofina coriônica equina (eCG); D9 -1,0 mg de BE e IATF após 36 horas; G2: D0 -P 4 intravaginal, mais 50 microgramas de hormônio liberador de gonadotrofina (GnRH); D7 -remoção da P 4 mais PGF2α mais 400 UI de eCG; D9 50 microgramas de GnRH e IATF 16 horas após; G3: Grupo controle inseminado 12 horas após a observação visual do estro. Resultados: No G1 e no G2 a IATF foi feita no D55,5±0.9 a no D56,8±1.3 após o parto, enquanto que no G3 a inseminação artificial foi executada no D84,5±5.7 (p<0,05). A taxa de prenhez no G1, G2 e G3 foi respectivamente de 47,0, 46,2 e 35,8 % (p=0,09). Conclusão: observou-se significativa redução do intervalo parto-concepção nas vacas tratadas assim como melhor taxa de prenhez (p=0.09) em relação ao grupo controle. Palavras-chave: período pós parto; taxa de prenhez; vacas leiteiras; progestero...

show abstract

“…Therefore, these results indicate that the CIDR-based TET protocol has an advantage that enables more cows per treated animals to be used as recipients and guarantees timely ovulation and concomitant CL formation. Other studies [29][30][31] have also shown that the P4-based TET scheme provides acceptable pregnancy rates (42.4-63.4%) in beef recipients.…”

Section: Discussionmentioning

confidence: 90%

A CIDR-Based Timed Embryo Transfer Protocol Increases the Pregnancy Rate of Lactating Repeat Breeder Dairy Cows

Son

Choe

Cho

et al. 2007

Journal of Reproduction and Development

View full text Add to dashboard Cite

Abstract. This study evaluated the pregnancy rates following either a controlled internal drug release (CIDR)-based timed artificial insemination (TAI) or an embryo transfer (TET) protocol compared with that following a single PGF2α injection and AI after estrus (AIE) in lactating repeat breeder dairy cows. Fifty-three lactating dairy cows diagnosed as repeat breeders were used in this study and were randomly assigned to the following three treatments. (1) Cows, at random stages of the estrous cycle, received a CIDR device and 2 mg estradiol benzoate (EB; Day 0), a 25 mg PGF2α injection at the time of CIDR removal on Day 7 and a 1 mg EB injection on Day 8. The cows then received TAI 30 h (Day 9) after the second EB injection using dairy semen (TAI group, n=13). (2) Cows, at random stages of the estrous cycle, received the same hormonal treatments as in the TAI group. The cows then received TET on Day 16 using frozen-thawed blastocysts or morula embryos collected from Korean native cattle donors (TET group, n=13). (3) Cows, at the luteal phase, received a 25 mg injection of PGF2α and AIE using dairy semen (control group, n=27). The ovaries of the cows in the TET group were examined by transrectal ultrasonography to determine ovulation of the preovulatory follicles, and blood samples were collected for serum progesterone (P4) analysis. The pregnancy rate was significantly higher in the TET group (53.8%) than in the control (18.5%) or TAI (7.7%) groups (P<0.05). The ultrasonographic observations demonstrated that all the cows in the TET group ovulated the preovulatory follicles and concomitantly formed new corpora lutea. Accordingly, the mean serum P4 concentration remained constant between Day 0 and Day 7 of the luteal phase, decreased dramatically on Day 8 (P<0.01) and subsequently increased by Day 16 (P<0.01). These data suggest that the CIDR-based TET protocol can be used to effectively increase the pregnancy rate in lactating repeat breeder dairy cows. Key words: Controlled internal drug release (CIDR), Lactating repeat breeder dairy cow, Pregnancy rate, Timed artificial insemination (TAI), Timed embryo transfer (TET) (J. Reprod. Dev. 53: [1313][1314][1315][1316][1317][1318] 2007) epeat breeding is a major cause of bovine infertility. Repeated inseminations, delayed conception and increased culling have a severe economic impact on dairy farms associated with repeat breeding [1]. The various problems that cause bovine infertility and delayed conception include reproductive tract abnormalities, estrous detection

show abstract

Comparison of four synchronization protocols for fixed-time bovine embryo transfer in Bos indicus × Bos taurus recipients

Cited by 38 publications

References 11 publications

Immune-serological identification of infectious agents with influence on bovine embryo transfer in the north of Brazil

Immune-serological identification of infectious agents with influence on bovine embryo transfer in the north of Brazil

Reduction of the Voluntary Waiting Period by Use of Hormonal Protocols and Fixed-Time Artificial Insemination (Ftai) in Dairy Cows

A CIDR-Based Timed Embryo Transfer Protocol Increases the Pregnancy Rate of Lactating Repeat Breeder Dairy Cows

Contact Info

Product

Resources

About