Comparison of four techniques for the routine diagnosis of Trichomonas vaginalis infection.

Abstract

SYNOPSIS Specimens from 495 patients attending Johannesburg hospitals and family planning clinics were examined for Trichomonas vaginalis by microscopy of Giemsa (GS), Papanicolaou (Pap), and acridine-orange (AO) stained smears, and by culture in Feinberg-Whittington medium. Culture, Pap and GS stained smears from vaginal swabs yielded fewer positives than AO stained smears. Although Pap-stained cytological smears gave the highest number of posit,ves, in 300% of these cases the presence of T.vaginalis could not be confirmed by examination of vaginal swabs.Of the positive AO-stained smears, 93 % were also positive by at least one other technique.

Evaluation of Different Staining Techniques in the Diagnosis of Trichomonas vaginalis Infection in Females of Reproductive Age Group

“…This is in agreement with a previously done study which showed that the infection rate detected by acridine orange staining was higher than either culture or other vaginal swab examinations [19]. Although acridine orange staining is easy to perform, rapid and screening of the vaginal smears is done in less time, the major disadvantage of this technique is that it requires special microscopic facilities, trained personnel and availability of the dye.…”

“…Studies done by different workers have reported varying range of sensitivity (41-56%) of giemsa stained smears for the diagnosis of Trichomonas vaginalis infection [19,24,25]. In one study it was reported that giemsa staining had high sensitivity (100%) and specificity (99.69%) compared to culture.…”

“…It was then stained with giemsa stain (Hi Media Laboratories, India), diluted 1 part to 19 parts of 1/15M phosphate buffer, pH 7.2 for 10 min and scanned for Trichomonas vaginalis at 100X magnification [19]. Both the internal and external structures of the organism were clearly visualized.…”

Etiologic characterization of vulvovaginitis among females attending a tertiary care hospital: a one year study

“…22 Most common symptoms associated are thin green colored discharge with pH >5, Discharge was malodoured and Vulval itching was the found to be associated commonly. Findings of our study was consistent with the reports of S R Fule et al, Madhivanan P et al, and Mason P R et al [23][24][25] These differences in variation may be due to variations in personal hygiene, environment and socio economic conditions which vary from place to place.…”

Prevalence of Candida and Trichomonas in Vaginal Discharge of Rural Females not Seeking Medical Help: Microscopy and Its Role in Vaginitis

“…A very high prevalence of TV 47% was reported (28) . Studies have indicated it as reemerging pathogen and found to correlate with age.…”