Comparison of generational effect on proteins and metabolites in non-transgenic and transgenic soybean seeds through the insertion of the cp4-EPSPS gene assessed by omics-based platforms

Campos, Bruna Kauely de; Galazzi, Rodrigo Moretto; Santos, Bruna Marques dos; Balbuena, Tiago Santana; Santos, Fábio Neves dos; Mokochinski, João Benhur; Eberlin, Marcos N.; Arruda, Marco Aurélio Zezzi

doi:10.1016/j.ecoenv.2020.110918

Cited by 11 publications

(9 citation statements)

References 27 publications

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“…The same result was achieved by Harrigan et al [59] and Clarke et al [60]. However, it is reported that transgenic soybeans were less affected by generational effects and can present more secondary metabolites, such as prenylated isoflavones [61,62].…”

Section: Metabolomics and Soy An Overviewsupporting

confidence: 76%

Metabolomics as a Tool to Study Underused Soy Parts: In Search of Bioactive Compounds

Bragagnolo

Funari

Ibáñez

2021

Foods

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The valorization of agri-food by-products is essential from both economic and sustainability perspectives. The large quantity of such materials causes problems for the environment; however, they can also generate new valuable ingredients and products which promote beneficial effects on human health. It is estimated that soybean production, the major oilseed crop worldwide, will leave about 597 million metric tons of branches, leaves, pods, and roots on the ground post-harvesting in 2020/21. An alternative for the use of soy-related by-products arises from the several bioactive compounds found in this plant. Metabolomics studies have already identified isoflavonoids, saponins, and organic and fatty acids, among other metabolites, in all soy organs. The present review aims to show the application of metabolomics for identifying high-added-value compounds in underused parts of the soy plant, listing the main bioactive metabolites identified up to now, as well as the factors affecting their production.

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Section: Metabolomics and Soy An Overviewsupporting

confidence: 76%

Metabolomics as a Tool to Study Underused Soy Parts: In Search of Bioactive Compounds

Bragagnolo

Funari

Ibáñez

2021

Foods

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“…A lower number of protein spots were found in the callus cell line CF-23 with respect to wild-type CF-WT, indicating a different protein content ( Figure 3 ). Similar findings have been reported in other genetically modified plants ( Barbosa et al., 2012 ; Tan et al., 2017 ; de Campos et al., 2020 ). The CF-23 transgenic line produced two spots (41 and 50) whose MW and IP values resemble chymopapain, the main cysteine proteinase in papaya.…”

Section: Discussionsupporting

confidence: 91%

Improvement of cell suspension cultures of transformed and untransformed Carica papaya cell lines, towards the development of an antiparasitic product against the gastrointestinal nematode Haemonchus contortus

Caltempa

Hernández

Pérez

et al. 2022

Front. Cell. Infect. Microbiol.

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Parasitic diseases have a major impact on human and animal health worldwide. Despite the availability of effective anti-parasitic drugs, their excessive and uncontrolled use has promoted the emergence of drug resistance, severely affecting ecosystems and human health. Thus, developing environmentally friendly antiparasitic treatments is urgently needed. Carica papaya has shown promising effects against infectious diseases. C. papaya embryogenic calluses were genetically modified by our research team to insert immunogenic peptides with the goal of developing an oral anti-cysticercosis vaccine. Among these callus cell lines, one labeled as CF-23, which expresses the KETc7 immunogenic peptide, induced the highest protection levels against experimental cysticercosis. In the process of designing a natural antiparasitic product based on C. papaya that simultaneously induced immunity against cysticercosis, both transformed (SF-23) and untransformed (SF-WT) suspension cultures were produced and optimized. Our results showed a better duplication time (td) for SF-23 (6.9 days) than SF-WT (13.02 days); thus, the SF-23 line was selected for scale-up in a 2-L airlift bioreactor, reaching a td of 4.4 days. This is the first time that a transgenic line of C. papaya has been grown in an airlift bioreactor, highlighting its potential for scale-up cultivation in this type of reactor. Considering the previously reported nematocidal activity of C. papaya tissues, their activity against the nematode Haemonchus contortus of aqueous extracts of SF-WT and SF-23 was explored in this study, with promising results. The information herein reported will allow us to continue the cultivation of the transgenic cell suspension line of C. papaya under reproducible conditions, to develop a new anti-parasitic product.

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“…The average correlation coefficients were 0.91 ± 0.2 for the non-GM sample and 0.92 ± 0.2 for the GM sample with a total number of matched spots of 514 and 669 for the non-GM and GM gels, respectively ( Table 1 ). These results indicate a high degree of sensitivity and reproducibility using the 2D-DIGE/MS approach (Choudhary et al, 2016 ; de Campos et al, 2020 ).…”

Section: Resultsmentioning

confidence: 86%

Untargeted Proteomics-Based Approach to Investigate Unintended Changes in Genetically Modified Maize for Environmental Risk Assessment Purpose

et al. 2021

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Profiling technologies, such as proteomics, allow the simultaneous measurement and comparison of thousands of plant components without prior knowledge of their identity. The combination of these non-targeted methods facilitates a more comprehensive approach than targeted methods and thus provides additional opportunities to identify genotypic changes resulting from genetic modification, including new allergens or toxins. The purpose of this study was to investigate unintended changes in GM Bt maize grown in South Africa. In the present study, we used bi-dimensional gel electrophoresis based on fluorescence staining, coupled with mass spectrometry in order to compare the proteome of the field-grown transgenic hybrid (MON810) and its near-isogenic counterpart. Proteomic data showed that energy metabolism and redox homeostasis were unequally modulated in GM Bt and non-GM maize variety samples. In addition, a potential allergenic protein—pathogenesis related protein −1 has been identified in our sample set. Our data shows that the GM variety is not substantially equivalent to its non-transgenic near-isogenic variety and further studies should be conducted in order to address the biological relevance and the potential risks of such changes. These finding highlight the suitability of unbiased profiling approaches to complement current GMO risk assessment practices worldwide.

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Comparison of generational effect on proteins and metabolites in non-transgenic and transgenic soybean seeds through the insertion of the cp4-EPSPS gene assessed by omics-based platforms

Cited by 11 publications

References 27 publications

Metabolomics as a Tool to Study Underused Soy Parts: In Search of Bioactive Compounds

Metabolomics as a Tool to Study Underused Soy Parts: In Search of Bioactive Compounds

Improvement of cell suspension cultures of transformed and untransformed Carica papaya cell lines, towards the development of an antiparasitic product against the gastrointestinal nematode Haemonchus contortus

Untargeted Proteomics-Based Approach to Investigate Unintended Changes in Genetically Modified Maize for Environmental Risk Assessment Purpose

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