Comparison of Gut Microbiota between Lean and Obese Adult Thai Individuals

Jinatham, Vasana; Kullawong, Niwed; Kespechara, Kongkiat; Gentekaki, Eleni; Popluechai, Siam

doi:10.4014/mbl.1711.11003

Cited by 10 publications

(14 citation statements)

References 32 publications

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“…Colonies were then visually selected based on morphologies that were compatible with the target species: small, flat colonies encrusted in the agar plates for F. prausnitzii , and tiny bulging colonies for C. minuta . They were then screened using previously described PCR primers: Fprau 02 (5′-GAG CCT CAG CGT CAG TTG GT-3′) and Fprau 07 (5′-CCA TGA ATT GCC TTC AAA ACT GTT-3′) for F. prausnitzii [ 7 ], Yso F (5′-CCC ACC AAG TCAA CGA TGG G-3′), and ChrisM-R1 (5′-CCC TCT CCT GTA CTC AAG TC-3′) for C. minuta [ 54 ] and this study.…”

Section: Methodsmentioning

confidence: 99%

Species-targeted sorting and cultivation of commensal bacteria from the gut microbiome using flow cytometry under anaerobic conditions

et al. 2022

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Background There is a growing interest in using gut commensal bacteria as “next generation” probiotics. However, this approach is still hampered by the fact that there are few or no strains available for specific species that are difficult to cultivate. Our objective was to adapt flow cytometry and cell sorting to be able to detect, separate, isolate, and cultivate new strains of commensal species from fecal material. We focused on the extremely oxygen sensitive (EOS) species Faecalibacterium prausnitzii and the under-represented, health-associated keystone species Christensenella minuta as proof-of-concept. Results A BD Influx® cell sorter was equipped with a glovebox that covered the sorting area. This box was flushed with nitrogen to deplete oxygen in the enclosure. Anaerobic conditions were maintained during the whole process, resulting in only minor viability loss during sorting and culture of unstained F. prausnitzii strains ATCC 27766, ATCC 27768, and DSM 17677. We then generated polyclonal antibodies against target species by immunizing rabbits with heat-inactivated bacteria. Two polyclonal antibodies were directed against F. prausnitzii type strains that belong to different phylogroups, whereas one was directed against C. minuta strain DSM 22607. The specificity of the antibodies was demonstrated by sorting and sequencing the stained bacterial fractions from fecal material. In addition, staining solutions including LIVE/DEAD™ BacLight™ Bacterial Viability staining and polyclonal antibodies did not severely impact bacterial viability while allowing discrimination between groups of strains. Finally, we combined these staining strategies as well as additional criteria based on bacterial shape for C. minuta and were able to detect, isolate, and cultivate new F. prausnitzii and C. minuta strains from healthy volunteer’s fecal samples. Conclusions Targeted cell-sorting under anaerobic conditions is a promising tool for the study of fecal microbiota. It gives the opportunity to quickly analyze microbial populations, and can be used to sort EOS and/or under-represented strains of interest using specific antibodies, thus opening new avenues for culture experiments.

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Section: Methodsmentioning

confidence: 99%

Species-targeted sorting and cultivation of commensal bacteria from the gut microbiome using flow cytometry under anaerobic conditions

et al. 2022

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“…Height, weight, and waist circumference were measured and body mass index (BMI) was calculated (kg/m 2 ).The participants were classified into normal weight and healthy obese groups according to the criteria of the WHO for adults. Body mass index of normal weight was from 18.5 to 24.99 kg/m 2 , and for the obese was ≥ 30 kg/m 2 [7,13].…”

Section: Anthropometric Measurementsmentioning

confidence: 99%

“…Obesity is a growing health problem and global challenge for health care [6,7]. Healthy obesity or metabolically healthy obese is a subtype of obesity which means obese individuals remain free of metabolic health complications such as type 2 diabetes mellitus and cardiovascular disease.…”

Section: Introductionmentioning

confidence: 99%

The relationship between the gut microbiota shifts and the inflammatory biomarkers in obese and normal weight adults

Mohammed

Hosny

Mohamed

et al. 2022

Microbes and Infectious Diseases

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Background: Healthy obesity means obese individuals remain free of metabolic health complications. Several researches link microbiota changes to obesity, focus on metabolic role of IL-6 and its ability to alter fat metabolism and correlate circulating calprotectin concentration to morbid obesity. This study evaluated the relative abundance of gut microbiota (Prevotella and γ-Proteobacteria), estimated the serum levels of inflammatory mediators (IL-6 and calprotectin) and studied the correlation between them in healthy obese and normal weight adults. Methods: 33 healthy obese (HO) and 14 normal weight (NW) controls were included. Serum levels of IL-6 and calprotectin were estimated by ELISA. Abundances of Prevotella and γ-Proteobacteria were determined in stool using real time PCR. Results: IL-6 and calprotectin levels were significantly lower in HO than in NW (p=0.001, p=0.001 respectively) with significant negative correlation between IL-6 and body mass index (BMI) in HO (r=-0.438, p=0.011) and negative but statistically insignificant correlation with BMI in NW groups (r=-0.024, p=0.935). There was negative but statistically insignificant correlation between calprotectin and BMI in HO (r=-0.075, p=0.677) and NW (r=-0.381, p=0.179). Prevotella and γ-Proteobacteria abundances were higher in HO than in NW and insignificant (p=0.31, p=0.55 respectively) with significant positive correlation between Prevotella and γ-Proteobacteria abundance in HO (r=0.436, p=0.011), positive and insignificant correlations between abundance of Prevotella and levels of IL-6 in HO and NW (r=0.303, P=0.086 vs r=0.316, p=0.272 respectively). Conclusion: IL-6 and calprotectin have role in regulation of energy homeostasis. Elevated abundances of Prevotella and γ-Proteobacteria may be the primary shifts in gut microbiota of HO.

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“… Streptococcaceae was associated with those individuals with higher BMI. Ottosson et al., 2018 [ 85 ] Sequencing 674 individuals (lean, overweight, obese) Positive correlation between BMI and Lachnospiraceae (Blautia, Dorea and Ruminococcus), and negative correlation between BMI and SHA-98 Jinatham et al., 2018 [ 84 ] qPCR 42 individuals Lean n = 21, overweight n = 10, obese n = 11 Bacteroidetes, Firmicutes, Staphylococcus, Akkermansia muciniphila, Methanobacteria: lower in individuals with obesity, compared with lean. Ruminoccocus, Christensenella minuta, γ-Proteobacteria, Akkermansia Municiphila: lower in individuals with obesity, compared with overweight.…”

Section: Gut Microbiota and Its Close Relationship To Dietmentioning

confidence: 99%

“…were found in higher concentrations in obese and overweight children than in lean ones. Also, individuals with obesity showed lower counts for Bacteroidetes, Firmicutes, Staphylococcus, Akkermansia muciniphila, Methanobacteria, compared with lean individuals and lower counts for Ruminoccocus, Christensenella minuta, γ-Proteobacteria, Akkermansia municiphila, compared with overweight [ 84 ]. Verrucomicrobia phylum was significantly more abundant in the normal weight group.…”

Section: Obesity and The Perspective Of Gut Microbiotamentioning

confidence: 99%

Gut microbiome and Mediterranean diet in the context of obesity. Current knowledge, perspectives and potential therapeutic targets

et al. 2021

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Mediterranean Diet has been recognized as one of the healthiest and sustainable dietary patterns worldwide, based on the food habits of people living in the Mediterranean region. It is focused on a plant-based cuisine combining local agricultural products and moderate intake of fish. As eating habits seem to exert a major impact on the composition of gut microbiota, numerous studies show that an adherence to the Mediterranean diet positively influences the microbiome ecosystem network. This has a profound effect on multiple host metabolic pathways and plays a major role in immune and metabolic homeostasis. Among metabolic disorders, obesity represents a major health issue where Mediterranean Dietary regime could possibly slowdown its spread. The aim of this review is to emphasize the interaction between diet and gut microbiota and the potential beneficial effects of Mediterranean diet on metabolic disorders like obesity, which is responsible for the development of many noncommunicable diseases.

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Comparison of Gut Microbiota between Lean and Obese Adult Thai Individuals

Cited by 10 publications

References 32 publications

Species-targeted sorting and cultivation of commensal bacteria from the gut microbiome using flow cytometry under anaerobic conditions

Species-targeted sorting and cultivation of commensal bacteria from the gut microbiome using flow cytometry under anaerobic conditions

The relationship between the gut microbiota shifts and the inflammatory biomarkers in obese and normal weight adults

Gut microbiome and Mediterranean diet in the context of obesity. Current knowledge, perspectives and potential therapeutic targets

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