Comparison of histometric and morphometric analyses of bone height in ligature-induced periodontitis in rats

Abstract

The purpose of this study was to compare histologic and morphometric procedures of bone height measurement. Microscopic measurements are the most frequent methods in periodontal studies with animals, but have limited capacity to identify bone levels associated with both healthy tissues and periodontal disease. Ligatures were placed in the maxillary left second molars of 10 male 60-day-old Wistar rats for 30 days. Left and right maxillary sides of 5 rats were processed for histologic analysis (H), sectioned buccolingually, and stained with HE. The maxillae of the other 5 rats were defleshed and used for morphometric analysis (M). Histometric measurements from the cementoenamel junction to the bone crest were performed. Standardized photographs were used for morphometric analysis. The t test was used for dependent or independent samples (alpha = 0.05%). Distances from cementoenamel junction to bone crest were 0.95 +/- 0.25 and 1.07 +/- 0.30 mm for H and M, respectively. Buccal measurements were 0.92 +/- 0.16 and 1.08 +/- 0.35 mm for H and M. The values obtained using H and M for areas without ligatures were 0.44 +/- 0.15 and 0.47 +/- 0.11 mm for lingual measurements and 0.23 +/- 0.08 and 0.41 +/- 0.10 mm for buccal measurements. No significant differences were found between the two methods in the detection of bone height associated with the placement of ligatures in rats.

Influence of different durations of estrogen deficiency on alveolar bone loss in rats

“…20 Morphometric and histologic procedures were compared for alveolar bone measurement, and no significant differences were found between the two methods when the amount of bone was measured after ligature placement in rats. 21 A recent study 22 morphometrically evaluated bone loss by comparing different locations in experimental periodontitis in rats. The maxilla showed greater bone loss than the mandible, and the bone loss was greater on the buccal side.…”

Effect of glutamine ingestion on the progression of induced periodontitis: experimental study in rats

“…Glutamine supplementation is commonly used in health care 16 . Its use in the form of supplementation is relevant in patients experiencing polytrauma who are hospitalized in the intensive care unit and patients with cancer and other enteral tract disorders.…”

“…There are several works in the literature that validate all these findings [20][21][22] . In 2007, Fernandes et al 16 conducted an experimental study to compare a morphometric analysis with an histological analysis to verify if there was a difference between the methods. For the study, 10 Wistar rats were selected and divided into two groups; periodontal disease was induced in all animals and at the end, their jaws were removed.…”

Evaluation of the effect of an organic extract obtained from Ipomoea alba L. on experimental periodontitis in rats

“…To validate measurement conversions, a millimetric ruler was photographed together with all specimens. 17 Alveolar bone loss was determined on the buccal surface of the mandibular first molars by the distance of the CEJ from the alveolar bone crest (ABC). Measurements were made along the axis of each root in three regions of the first molar (three roots) (Figure 1).…”