Comparison of immunoreaction between two commercial antibodies against 14-3-3<i>σ</i>protein in canine tissues

Suárez‐Bonnet, Alejandro; Aguirre-Sanceledonio, M.; Andrada, M.; Herráez, P.; Quesada‐Canales, Óscar; Monteros, A. Espinosa de los

doi:10.1080/09712119.2015.1013963

Cited by 2 publications

(3 citation statements)

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“…[33][34][35] In the current study, immunohistochemical analysis revealed the absence of 14-3-3s immunolabeling in normal kidney and is in accordance with previous studies using Western blot and immunohistochemistry in both humans and dogs. 22,24,34,36 Interestingly, 14-3-3s was neoexpressed in 37.5% of canine RCCs, which is more than double the 16.4% rate described in human RCCs. 22 Additionally, positive cases that had vascular invasion showed positive labeling in embolic neoplastic cells.…”

Section: Discussionmentioning

confidence: 89%

“…These results are compatible with previous studies in human and canine mammary basal carcinomas, characterized by a poor prognosis, that also overexpress 14-3-3s. 16,36 It has now been established that 14-3-3s can promote lung, liver, and pancreatic carcinomas. 31,32,38,39 Similar to our findings, these tumors arise from tissues that do not constitutively express the protein.…”

Section: Discussionmentioning

confidence: 99%

“…The 14-3-3s antibody has been previously shown to identify the appropriate canine molecular weight antigens by Western blot and to cross-react with canine tissues. 27,34,36 Heat-induced antigen retrieval was performed using a pH 6 buffer (Bond ER1; Leica) for 10 minutes (vimentin), 20 minutes (14-3-3s), and 30 minutes (AE1/AE3) at 90 C and a pH 9 buffer (Bond ER2; Leica) for 10 minutes at 90 C for Ki-67. The Bond Polymer Refine Detection kit (Leica) was used for visualization with hematoxylin counterstain.…”

Section: Immunohistochemistry and Evaluationmentioning

confidence: 99%

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14-3-3σ Protein Expression in Canine Renal Cell Carcinomas

et al. 2017

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14-3-3σ is a protein expressed in many epithelial tissues associated with essential cell functions, including cell-cycle control, apoptosis, and cytoskeletal integrity. There is a paucity of knowledge of the tumorigenesis of canine renal cell carcinomas (RCCs), and the histological origin of this tumor has not been established. This study analyzed the expression of 14-3-3σ, Ki-67, cytokeratins, and vimentin in 40 canine RCCs. Aberrant expression of 14-3-3σ was demonstrated in 15 (38%) cases and was associated with a significantly shorter survival time ( P < .002). In contrast to canine RCC, normal kidney did not express 14-3-3σ. The Ki-67 proliferation index did not show utility as a prognostic factor. The distal convoluted tubular epithelium in normal kidneys coexpressed cytokeratins and vimentin, and thus maintenance of this coexpression pattern in canine RCC suggests that most tumors arise from the distal segment of the nephron. These results suggest that 14-3-3σ is a potential negative prognostic factor and a possible therapeutic target.

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