Comparison of Impregnated Bone Morphogenetic Protein-2 Release Kinetics from Biopolymer Scaffolds

Vasilyev, A. V.; Бухарова, Т. Б.; Кузнецова, В. С.; Zagoskin, Yu. D.; Минаева, С. А.; Grigoriev, T. E.; Антонов, Е. Н.; Osidak, E. O.; Galitsyna, Elena V.; Бабиченко, И. И.; Domogatsky, S.P.; Попов, В. К.; Чвалун, С. Н.; Goldshtein, D. V.; Aa, Kulakov

doi:10.1134/s2075113319050332

Cited by 13 publications

(15 citation statements)

References 23 publications

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“…In our view, this could be related to features of impregnation technology and release kinetics of BMP-2. As shown previously, BMP-2 losses in the collagen-fibronectin hydrogel during protein release was 11% less than in the PLA granules [12]. Additionally, hydrogel had a larger contact area with the surrounding tissues than PLA granules.…”

Section: Discussionsupporting

confidence: 63%

“…As we have shown earlier, highly porous polylactide granules could be a good filler. They can release small doses of BMP-2 for a long time, provide ectoand orthotopic neo-ostegenesis, and exclude possible complications associated with an excessive concentration of BMP-2: hyperostosis, inflammation, and anaplasia [12,13].…”

Section: Introductionmentioning

confidence: 99%

“…Fibronectin was used to promote the slow release of BMP-2 from the collagen hydrogel with HAP/β-TCP filling. Its competitive adhesion leads to the protein's slow release from the collagen-BMP-2 complex [12,15].…”

Section: Introductionmentioning

confidence: 99%

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Osteoinductive Moldable and Curable Bone Substitutes Based on Collagen, BMP-2 and Highly Porous Polylactide Granules, or a Mix of HAP/β-TCP

Vasilyev

Кузнецова

Бухарова

et al. 2021

Preprint

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In dentistry, maxillofacial surgery, traumatology, and orthopedics, there is a need to use osteoplastic materials that have not only osteoinductive and osteoconductive properties but are also convenient for use. In the study, compositions based on collagen hydrogel were developed. Polylactide granules (PLA) or a traditional bone graft, a mixture of hydroxyapatite and β-tricalcium phosphate (HAP/β-TCP), were used for gel filling to improve mechanical osteoconductive properties of compositions. The mechanical tests showed that collagen hydrogels filled with 12 wt% highly porous PLA granules (elastic modulus 373 ± 55 kPa) or 35 wt% HAP/β-TCP granules (elastic modulus 451 ± 32 kPa) had optimal manipulative properties. All composite components were cytocompatible. The cell’s viability was above 90%, and the components’ structure facilitated the cell’s surface adhesion. The bone morphogenetic protein-2 (BMP-2) provided osteoinductive composition properties. It was impregnated directly into the collagen hydrogel with the addition of fibronectin or inside porous PLA granules. The implantation of a collagen hydrogel with BMP-2 and PLA granules into a critical-size calvarial defect in rats led to the formation of the most significant volume of bone tissue: 61 ± 15%. It was almost 2.5 times more than in the groups where a collagen-fibronectin hydrogel with a mixture of HAP/β-TCP (25 ± 7%) or a fibronectin-free composition with porous PLA granules impregnated with BMP-2 (23 ± 8%) were used. Subcutaneous implantation of the compositions also showed their high biocompatibility and osteogenic potential in the absence of a bone environment. Thus, the collagen-fibronectin hydrogel with BMP-2 and PLA granules has optimal biocompatibility, osteogenic, and manipulative properties.

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Section: Discussionsupporting

confidence: 63%

Section: Introductionmentioning

confidence: 99%

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Osteoinductive Moldable and Curable Bone Substitutes Based on Collagen, BMP-2 and Highly Porous Polylactide Granules, or a Mix of HAP/β-TCP

Vasilyev

Кузнецова

Бухарова

et al. 2021

Preprint

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“…Implantation into critical-size calvarial defects: Operations were performed following the previously developed method [ 12 ]. After shaving and disinfection, transverse and vertical laterally displaced incisions of the scalp were made, forming a triangular flap.…”

Section: Methodsmentioning

confidence: 99%

“…Small doses of BMP-2 induce osteogenesis due to prolonged release. Prolonged release should help reduce the risks that may be associated with excessive BMP-2 concentration: hyperostosis, inflammation, and anaplasia [ 12 , 13 ]. The use of PLA granules in combination with a chitosan–glycerophosphate hydrogel will improve the resorption of chitosan by proton acceptance during the resorption of polylactides.…”

Section: Introductionmentioning

confidence: 99%

Influence of the Degree of Deacetylation of Chitosan and BMP-2 Concentration on Biocompatibility and Osteogenic Properties of BMP-2/PLA Granule-Loaded Chitosan/β-Glycerophosphate Hydrogels

et al. 2021

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Compositions based on chitosan/β-glycerophosphate hydrogels with highly porous polylactide granules can be used to obtain moldable bone graft materials that have osteoinductive and osteoconductive properties. To eliminate the influence of such characteristics as chain length, degree of purification, and molecular weight on a designed material, the one-stock chitosan sample was reacetylated to degrees of deacetylation (DD%) of 19.5, 39, 49, 55, and 56. A study of the chitosan/β-glycerophosphate hydrogel with chitosan of a reduced DD% showed that a low degree of deacetylation increased the MSCs (multipotent stromal cells) viability rate in vitro and reduced the leukocyte infiltration in subcutaneous implantation to Wistar rats in vivo. The addition of 12 wt% polylactide granules resulted in optimal composite mechanical and moldable properties, and increased the modulus of elasticity of the hydrogel-based material by approximately 100 times. Excessive filling of the material with PLA (polylactide) granules (more than 20%) led to material destruction at a ~10% strain. Osteoinductive and osteoconductive properties of the chitosan hydrogel-based material with reacetylated chitosan (39 DD%) and highly porous polylactide granules impregnated with BMP-2 (bone morphogenetic protein-2) have been demonstrated in models of orthotopic and ectopic bone formation. When implanted into a critical-size calvarial defect in rats, the optimal concentration of BMP-2 was 10 μg/mL: bone tissue areas filled the entire material’s thickness. Implantation of the material with 50 μg/mL BMP-2 was accompanied with excessive growth of bone tissue and material displacement beyond the defect. Significant osteoinductive and osteoconductive properties of the material with 10 μg/mL of BMP-2 were also shown in subcutaneous implantation.

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Porous chitosan particles doped by in situ formed silver nanoparticles: Electrorheological response in silicon oil

Kuznetsov

Kovaleva

Volkov

et al. 2022

Polymers for Advanced Techs

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Effect of in situ formed silver nanoparticles doping on electrorheological response of highly porous chitosan particles in suspensions of polydimethylsiloxane (silicone oil) is considered. Silver nanoparticles are directly reduced by chitosan from solution. Highly porous chitosan particles with different silver content are fabricated by spraying from solution followed by freeze‐drying. A high and stable electrorheological response of suspensions in wide range of electric field strength is observed at a very low filler content of 1 wt%. The nature of the electrorheological effect is considered from the standpoint of dielectric spectroscopy. The activation energies of polarization processes are determined from the temperature dependences of the dielectric loss modulus. The study shows the opportunity to control the properties of stimuli‐responsive materials by changing the structure and physicochemical properties of the functional filler. This approach opens up new possibilities for creating materials with high performance and predetermined properties.

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Comparison of Impregnated Bone Morphogenetic Protein-2 Release Kinetics from Biopolymer Scaffolds

Cited by 13 publications

References 23 publications

Osteoinductive Moldable and Curable Bone Substitutes Based on Collagen, BMP-2 and Highly Porous Polylactide Granules, or a Mix of HAP/β-TCP

Osteoinductive Moldable and Curable Bone Substitutes Based on Collagen, BMP-2 and Highly Porous Polylactide Granules, or a Mix of HAP/β-TCP

Influence of the Degree of Deacetylation of Chitosan and BMP-2 Concentration on Biocompatibility and Osteogenic Properties of BMP-2/PLA Granule-Loaded Chitosan/β-Glycerophosphate Hydrogels

Porous chitosan particles doped by in situ formed silver nanoparticles: Electrorheological response in silicon oil

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