Comparison of IMS Platforms for Detecting and Recovering Escherichia coli O157 and Shigella flexneri in Foods

Lau, Henry K.; Clotilde, Laurie M.; Lin, Andrew; Hartman, Gary L.; Lauzon, Carol R.

doi:10.1177/2211068212468583

Cited by 13 publications

(5 citation statements)

References 4 publications

(4 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus far, the MagNA Pure96 platform (Roche, Basel, Switzerland) verified by previous studies is an accurate standard protocol [15]. However, careGENE TM Viral/Pathogen HiFi Nucleic Acid Isolation kit (WELLS BIO Inc., Seoul, Korea) on the KingFisher Flex platform (Thermo Fisher Scientific, Rocklin, CA, USA) and the SGRespi TM Pure kit (Seegene Inc., Seoul, Korea) on the Maelstrom 9600 platform (Taiwan Advanced Nanotech Inc., Taoyuan, Taiwan) have been recently developed; it is suggested that these novel extraction systems and platforms may extract nucleic acids as accurately as, but more rapidly than, the existing MagNA Pure96 platform [16][17][18]. Therefore, herein, the efficiency and extraction performance of these new extraction systems and platforms were evaluated and compared with those of the MagNA Pure 96 platform for the detect of SARS-CoV-2 in 245 respiratory specimens (NPS, sputum, and saliva).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Three Automated Extraction Systems for the Detection of SARS-CoV-2 from Clinical Respiratory Specimens

Lim

Jung²,

Park³

et al. 2022

Life

View full text Add to dashboard Cite

Severe acute respiratory syndrome coronavirus (SARS-CoV-2) is highly contagious and causes coronavirus disease 2019 (COVID-19). Reverse transcription quantitative polymerase chain reaction (RT-qPCR) is the most accurate and reliable molecular assay to detect active SARS-CoV-2 infection. However, a rapid increase in test subjects has created a global bottleneck in testing capacity. Given that efficient nucleic acid extraction greatly affects reliable and accurate testing results, we compared three extraction platforms: MagNA Pure 96 DNA and Viral NA Small Volume kit on MagNA Pure 96 (Roche, Basel, Switzerland), careGENETM Viral/Pathogen HiFi Nucleic Acid Isolation kit (WELLS BIO Inc., Seoul, Korea) on KingFisher Flex (Thermo Fisher Scientific, Rocklin, CA, USA), and SGRespiTM Pure kit (Seegene Inc., Seoul, Korea) on Maelstrom 9600 (Taiwan Advanced Nanotech Inc., Taoyuan, Taiwan). RNA was extracted from 245 residual respiratory specimens from the different types of samples (i.e., NPS, sputum, and saliva) using three different kits. The 95% limits of detection of median tissue culture infectious dose per milliliter (TCID50/mL) for the MagNA Pure 96, KingFisher Flex, and Maelstrom 9600 were 0.37–3.15 × 101, 0.41–3.62 × 101, and 0.33–1.98 × 101, respectively. The KingFisher Flex platform exhibited 99.2% sensitivity and 100% specificity, whereas Maelstrom 9600 exhibited 98.3–100% sensitivity and 100% specificity. Bland–Altman analysis revealed a 95.2% concordance between MagNA Pure 96 and KingFisher Flex and 95.4% concordance between MagNA Pure 96 and Maelstrom 9600, indicating that all three platforms provided statistically reliable results. This suggests that two modifying platforms, KingFisher Flex and Maelstrom 9600, are accurate and scalable extraction platforms for large-scale SARS-CoV-2 clinical detection and could help the management of COVID-19 patients.

show abstract

Section: Introductionmentioning

confidence: 99%

Evaluation of Three Automated Extraction Systems for the Detection of SARS-CoV-2 from Clinical Respiratory Specimens

Lim

Jung²,

Park³

et al. 2022

Life

View full text Add to dashboard Cite

show abstract

“…This still needs a long enrichment and complicated analysis procedures [23,24]. The KingFisher Flex Magnetic Particle Processor is also commercialized but the small sample volume is unfit for food safety on the basis of Food Standards [25,26]. And the TECTA™ B16 Automated Microbiology System is designed to detect E. coli O157:H7 and total coliforms from water samples.…”

Section: Introductionmentioning

confidence: 99%

Detection of Escherichia coli O157:H7 Using Automated Immunomagnetic Separation and Enzyme-Based Colorimetric Assay

Park

et al. 2020

Sensors

View full text Add to dashboard Cite

The food industry requires rapid and simple detection methods for preventing harm from pathogenic bacteria. Until now, various technologies used to detect foodborne bacteria were time-consuming and laborious. Therefore, we have developed an automated immunomagnetic separation combined with a colorimetric assay for the rapid detection of E. coli O157:H7 in food samples. The colorimetric detection method using enzymatic reaction is fascinating because of its simplicity and rapidity and does not need sophisticated devices. Moreover, the proposed procedures for the detection of bacteria in food take less than 3 h including pre-enrichment, separation and detection steps. First, target-specific immunomagnetic beads were introduced to contaminated milk in a pre-enrichment step. Second, the pre-enriched sample solution containing target bacteria bound on immunomagnetic beads was injected into an automated pretreatment system. Subsequently, the immunomagnetic beads along with target bacteria were separated and concentrated into a recovery tube. Finally, released β-galactosidase from E. coli O157:H7 after lysis was reacted with chlorophenol red β-galactopyranoside (CPRG) used as a substrate and the colorimetric change of CPRG was determined by absorbance measuring or the naked eye. By the proposed approach in this study, we could detect 3 × 102 CFU/mL of E. coli O157:H7 from a milk sample within 3 h.

show abstract

“…Besides antibody–antigen interaction, ligands such as aptamers (Joshi and others ; Wu and others 2014a), bacteriophage proteins (Poshtiban and others ), and other binding molecules (El‐Boubbou and others ) have been attached to magnetic beads for bacteria or bacterial nucleic acid (Zhang and others ; Li and others 2011b) separation in a similar way as IMS. The magnetic‐based separations have the advantages of specific removal of the target from food particles, inhibitors, and other organisms using rapid and easy manipulation with a common separation time within 1 h (Joshi and others ; Wang and others ; Poshtiban and others ; Cloutier and others ; Wang and others ), good recovery of the target (Uyttendaele and others ; El‐Boubbou and others ; Ravindranath and others ; Yang and others ; Wang and others ), no dilution of the sample or loss of carrier during washing, and possible automation and scale‐up separation (Horák and others ; Lau and others ; Chen and others ). The efficiency and selectivity of magnetic separation greatly depends on the ligands, as sometimes it is hard to obtain a ligand with high affinity and specificity to the target.…”

Section: Noncultural Target Separation and Concentration Methodsmentioning

confidence: 99%

Culture‐Independent Rapid Detection Methods for Bacterial Pathogens and Toxins in Food Matrices

Wang

Salazar

2015

Comp Rev Food Sci Food Safe

233

152

View full text Add to dashboard Cite

Rapid detection of bacterial pathogens and toxins in foods is necessary to provide real-time results to mitigate foodborne illness outbreaks. Cultural enrichment methods, although the most widely used, are time-consuming and therefore inadequate for rapid pathogen detection from food samples. The development of novel "rapid" detection methods has decreased detection time dramatically. This review presents an overview of detection methods for various foodborne pathogens, including Listeria monocytogenes, Salmonella enterica, and shiga toxin-producing Escherichia coli, and bacterial toxins in food matrices, with emphasis on those methods which do not require cultural enrichment. Discussed methods include nucleic acid-, immunological-, and biosensor-based techniques. A summary of each type of detection method is given, including referenced methods from the literature. Since these discussed methods do not require cultural enrichment, there is a higher probability of interference from the food matrices. Therefore, the review also discusses the potential interference of food components on detection methods and addresses preprocessing strategies to overcome matrix associated inhibition and to concentrate low quantities of pathogens and toxins in food. Development of rapid and sensitive detection technologies advances and ensures public health safety and security.

show abstract

Comparison of IMS Platforms for Detecting and Recovering Escherichia coli O157 and Shigella flexneri in Foods

Cited by 13 publications

References 4 publications

Evaluation of Three Automated Extraction Systems for the Detection of SARS-CoV-2 from Clinical Respiratory Specimens

Evaluation of Three Automated Extraction Systems for the Detection of SARS-CoV-2 from Clinical Respiratory Specimens

Detection of Escherichia coli O157:H7 Using Automated Immunomagnetic Separation and Enzyme-Based Colorimetric Assay

Culture‐Independent Rapid Detection Methods for Bacterial Pathogens and Toxins in Food Matrices

Contact Info

Product

Resources

About