Comparison of in vitro fertilization outcomes in ICSI cycles after human sperm
            preparation by density gradient centrifugation and direct micro swim-up without
            centrifugation

Palini, Simone; Stefani, Stefania; Primiterra, Mariangela; Benedetti, Serena; Barone, Stefano; Carli, Luca; Vaccari, Enrico; Ulug, M.; Feichtinger, Wilfried

doi:10.5935/1518-0557.20170022

Cited by 20 publications

(18 citation statements)

References 24 publications

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“…We reported herein that DSU technique did not show any significant difference in all ART outcomes as compared to the DGC-SU technique. However, Our results were inconsistent with the findings of Palini and colleagues in ICSI cycles [20]. Their results stated that blastulation rates per D3 embryos or per fertilized oocyte and pregnancy rates in DSU group were significant higher when compared to DGC-SU group.…”

Section: Discussioncontrasting

confidence: 99%

“…This finding suggests that density-gradient centrifugation should be avoided during sperm preparation [19]. In addition, the DGC-SU technique requires operators to transfer sperm sample among several tubes, thereby increasing the potential risk of mismatches [5,[20][21]. These limitations, in concert, suggested that the DGC-SU was not optimal technique for sperm preparation for IVF and ICSI treatments.…”

Section: Introductionmentioning

confidence: 99%

“…The DSU is a simple, fast and economical technique for sperm preparation without centrifugation. It has been reported that a micro direct swim-up without centrifugation (MSU) in ICSI treatment was a better choice for sperm preparation than the DGC-SU procedure [20][21]. However, there are no studies on whether this technique is a favorable choice for sperm preparation in conventional IVF treatment.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Direct swim-up without centrifugation is a more recommended technique for sperm preparation in conventional IVF cycles

Lu¹,

Jin²,

Xu³

et al. 2019

Preprint

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Backgroud: The aim of this study is to investigate the most effective and maneuverable technique for sperm preparation in conventional IVF cycles. Method: A retrospective and laboratory-based study was conducted in patients who underwent their first cycle of IVF from January to December in 2016 to compare two sperm preparation techniques: direct swim-up without centrifugation (DSU) technique and density-gradient centrifugation followed by swim-up (DGC-SU) technique. A series of experiments in this study was designed to evaluate the efficiency of these two techniques which include: (i) assessment of quality and quantity of spermatozoa by comparing motility, DNA fragmentation index (DFI), acrosomal reaction (AR) and mitochondrial membrane potential (MMP) of DSU-separated sperm to DGC-SU-separated sperm, (ii) evaluation of safety of DSU technique by assessing the risks of bacterial contamination, (iii) analysis of feasibility of replacing DGC-SU with DSU technique by reviewing ART outcomes including fertilization rate, high-quality embryo rate, implantation rate, clinical pregnancy rate, take-home baby rate and abortion rate. Results: Although there were no significant differences in DFI, AR and MMP between DSU-separated sperm and DGC-SU-separated sperm, significantly higher percentage of progressive motility in DSU-separated sperm were found than that in DGC-SU-separated sperm. Moreover, there were no significant differences between DSU and DGC-SU groups on ART outcomes based on data of fertilization rate, high-quality embryo rate, implantation rate, clinical pregnancy rate, baby delivery rate and abortion rate. In addition, no bacterial contaminations were found in culture medium samples of simulating fertilization from DSU group. However, it is noticeable that DSU technique required less time and labor for sperm preparation compared with DGC-SU.

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Section: Discussioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Direct swim-up without centrifugation is a more recommended technique for sperm preparation in conventional IVF cycles

Lu¹,

Jin²,

Xu³

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…The time saving of the investigated novel technique which is much faster than the classical sperm preparation procedure, is relevant particularly in avoiding the centrifugation steps using density gradients usually requiring up to 20 min, as well as subsequent centrifugations with the culture media to wash the pellet and remove any gradient compound that means more than 10 min, and finally the swim-up from pellet which takes normally between 40 and 50 min [ 26 , 27 ]. On the contrary, the novel procedure here reported, requires a total of 10 min to prepare the sperm, with a time saving of about 50 min compared with the direct swim-up procedure and further 75–80 min when including the density gradient step in the preparation.…”

Section: Discussionmentioning

confidence: 99%

A fast and safe technique for sperm preparation in ICSI treatments within a randomized controlled trial (RCT)

Baldini

Silvestris

et al. 2020

Reprod Biol Endocrinol

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Recently a novel method based on horizontal sperm migration in injection dishes has been introduced as an additional tool for preparation of semen sample in assisted reproductive technology (ART) procedures. In the present study, we evaluated both timing and reproductive outcomes in a randomized controlled study including 1034 intra-cytoplasmic sperm injection (ICSI) procedures followed by fresh embryo transfer. Couples enrolled were divided into two subgroups , namely conventional swim-up method (Group A), and horizontal sperm migration in injection dishes (Group B). No significant differences were found between groups with respect to fertilization rate, implantation success, clinical pregnancy outcomes and ongoing pregnancies. On the contrary, both cleavage and blastocyst rates were statistically higher in Group B, suggesting superior efficiency and safety of this innovative technique also including time-saving and cheaper costs as compared to the classical swim-up sperm preparation. Our data support the interpretation of the horizontal sperm migration as a promising procedure for semen preparation in ART cycles.

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“…Both techniques isolate a population of highly motile, morphologically normal sperm and result in similar ART outcomes. 75 However, the live birth rate following ICSI remains frustratingly static at 25-30% per (fresh) treatment cycle. 76 Various advanced sperm selection techniques have been proposed in the hope of improving this.…”

Section: New Developments In Sperm Selection In Artmentioning

confidence: 99%

An update on the management of male infertility

Karavolos

Panagiotopoulou²,

AlAhwany

et al. 2020

The Obstetric & Gynaecologis

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Male infertility underlies or contributes to up to 50% of infertility cases; current therapeutic interventions rely on assisted reproductive technology (ART), as medical or surgical treatments have limited value in enhancing semen quality or parameters. Lifestyle factors that affect male fertility could offer a therapeutic opportunity; however, their modification seems to be of variable benefit. In the quest for sperm functional assessment and selection tests, there is controversy over which patients, if any, should be tested for sperm DNA fragmentation, as well as which test to perform. Sperm selection techniques for intracytoplasmic sperm injection do not appear to significantly improve treatment outcomes or live birth rates. Routinely performed genetic tests are effective in determining aetiology in approximately 20% of infertile men; however, newer genetic tests could enhance diagnosis and change the future management of male infertility. Learning objectives To summarise the key lifestyle factors that affect male fertility. To appraise the currently available investigations for sperm testing and selection. To describe the genetic tests currently available to identify the aetiology of male infertility, including emerging technologies in the field of genetics and personalised genomics. Ethical issues How to deal with couples' requests for unproven medical interventions to manage male infertility?

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Comparison of in vitro fertilization outcomes in ICSI cycles after human sperm preparation by density gradient centrifugation and direct micro swim-up without centrifugation

Cited by 20 publications

References 24 publications

Direct swim-up without centrifugation is a more recommended technique for sperm preparation in conventional IVF cycles

Direct swim-up without centrifugation is a more recommended technique for sperm preparation in conventional IVF cycles

A fast and safe technique for sperm preparation in ICSI treatments within a randomized controlled trial (RCT)

An update on the management of male infertility

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