Comparison of Latex Agglutination With Enzyme Immunoassay for Detection of Rotavirus in Fecal Specimens

Abstract

Human rotaviruses are the most important etiologic agents of acquired diarrhea in infants and young children worldwide. Early diagnosis is essentialfor effective patient treatment. The latex agglutination (LA) assays for rotavirus diagnosis are rapid, inexpensive, and the most widely used to screen specimens. The performance of the LA Rotagen (Biokit S.A., Barcelona, Spain) was evaluated for rotavirus detection infecal samples of outpatients with acute gastroenteritis. This assay was compared with the enzyme immunoassay (EIA) EIARA (Bio-Manguinhos, Rio de Janeiro, Brazil). From January to October 2000, 285 fecal specimens were analyzed. Forty-four samples (15.4%) were reactive, 214 (75.4%) were nonreactive, and 27 (9.5%) were indeterminate by LA. All LA-positive samples were positive by EIA, and 2 LA-negative samples were positive by EIA. Of specimens indeterminate by LA, 67% were positive by EIA. The sensitivity, specificity, and accuracy of LA were 69%, 100%, and 93%, respectively. These results indicate that assay is as sensitive and specific as the EIA, and it could be applied on a large scale for screening stool specimens in suspected rotavirus diarrhea. However, the indeterminate results must be confirmed by other methods, such as EIA.

Rotavirus infection in a tertiary hospital: laboratory diagnosis and impact of immunization on pediatric hospitalization

“…Although this technique may be useful in certain situations, such as outbreaks of the disease, or in locations with lack of resources for RV detection, because it is fast, practical and has low cost. 31,50 A different distribution of positive samples was observed using this method in different years, with a progressive decline in the number of samples investigated since 2004. A study to assess the impact of vaccination against rotavirus has been previously conducted by our institution.…”

“…19,[26][27][28][29][30][31] Currently, several commercial tests are available from those based on less laborious techniques that enable a quick diagnosis, e.g. antigen detection methods, to more complex tests such as the polymerase chain reaction.…”

Comparative evaluation of antigen ELISA technique and PCR for detection of Rotavirus in stool samples of pediatric patients with diarrhea

“…Several decades after the rotavirus description many studies have been carried out to compare detection techniques (11,(16)(17)(18)(19)(20)(21) . Currently, several commercial tests are available from less laborious based techniques that enable a quick diagnosis, e.g.…”

Comparision of A Rapid Immunochromatography Test with Elisa to Detect Rotavirus

“…Raboni SM et al and Bahl R et al supported this observation of the present study. They reported that the majority of the cases of rotavirus diarrhea occurred in children younger than 2 years [16,17] .…”

“…Swab swirled ten times until the sample was dissolved into the assay diluents and swab was discarded while squeezing the swab against the wall of tube. [10][11][12][13][14][15][16][17][18][19][20] minutes.…”