Comparison of loop-mediated isothermal amplification and real-time PCR for the diagnosis of tuberculous pleurisy

Yang, Bo; Wang, X.; Li, H.; Li, G.; Cao, Zongjie; Cheng, Xuequn

doi:10.1111/j.1472-765x.2011.03141.x

Cited by 28 publications

(19 citation statements)

References 25 publications

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“…Slightly lower sensitivity in our study as compared to sputum sample could be due to the dilution of the organisms in the synovial fluid sample, as observed earlier by Negi et al Same factor could have contributed to sensitivity of 88.23% in IS6110 LAMP study on tuberculous meningitis, 70% in IS6110 LAMP study on tuberculous uveitis, and 85.7% in MPB64 LAMP study on intra‐ocular tuberculosis . Another study employing IS1081 LAMP assay on pleural fluid samples found a sensitivity and specificity of 48.7% and 83.3%, respectively . Such a low sensitivity can be explained by the presence of inhibitors in pleural fluid or the low copy number of IS1081 (5–7 repeats) in comparison to IS6110 (up to 25 repeats) …”

Section: Discussionsupporting

confidence: 47%

“…13 Another study employing IS1081 LAMP assay on pleural fluid samples found a sensitivity and specificity of 48.7% and 83.3%, respectively. 23 Such a low sensitivity can be explained by the presence of inhibitors in pleural fluid or the low copy number of IS1081 (5-7 repeats) in comparison to IS6110 (up to 25 repeats). 11,24 Uniquely, our study envisages the importance of using more than one target in the diagnosis of OATB.…”

Section: Discussionmentioning

confidence: 99%

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Diagnostic potential of multi‐targeted LAMP (loop‐mediated isothermal amplification) for osteoarticular tuberculosis

Sharma

Batra

et al. 2016

Journal Orthopaedic Research

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Delay in diagnosing osteoarticular tuberculosis (OATB) contributes significantly to morbidity by causing disfiguration and neurological sequelae. The delay caused by conventional culture and the expertise and expense involved in other nucleic acid based tests, make LAMP (loop-mediated isothermal amplification) assay a favorable middle path. We evaluated LAMP assay using IS6110 and MPB64 for rapid diagnosis of OATB by comparing with IS6110 PCR and culture. LAMP assay was performed on 140 synovial fluid and pus samples (10 culture-positive proven cases, 80 culture-negative probable cases, and 50 negative controls) using three set of primer pairs each for IS6110 and MPB64. LAMP assay, using two-target approach, had an overall sensitivity and specificity of 90% and 100% in detecting OATB. Sensitivity of IS6110 PCR, IS6110 LAMP, and MPB64 LAMP was 80%, 100%, and 100%, respectively, for confirmed cases and 72.5%, 81.75%, and 86.25%, respectively, for probable cases. Six additional cases were picked using two-target approach. LAMP assay utilizing IS6110 and MPB64 is a cost-effective technique for an early and reliable diagnosis of OATB. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:361-365, 2017.

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Section: Discussionsupporting

confidence: 47%

Section: Discussionmentioning

confidence: 99%

Diagnostic potential of multi‐targeted LAMP (loop‐mediated isothermal amplification) for osteoarticular tuberculosis

Sharma

Batra

et al. 2016

Journal Orthopaedic Research

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“…In this study, we evaluated LAMP, Xpert and SAT-TB assays for the diagnosis of TBP in PE culture positive patients, compared with PE smear and found that all these methods were suboptimal for the detection of MTB in PE, whereas each of them demonstrated high specificity. [27]. In a meta-analysis of 40 studies of NAATs for TBP, PAI et al reported that these tests had low sensitivities (43-77%), but high specificities (95%) [28].…”

Section: Discussionmentioning

confidence: 99%

Diagnostic performance of nucleic acid tests in tuberculous pleurisy

2020

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Background: Tuberculous pleurisy (TBP) is the most common form of extrapulmonary tuberculosis (TB). However, rapid diagnostic methods with high accuracy for tuberculous pleurisy are urgently needed. In the present study, we evaluated the diagnostic accuracy of Xpert MTB/RIF, LAMP and SAT-TB assay with pleural fluids from culture-positive TBP patients. Methods:We prospectively enrolled 300 patients with exudative pleural effusions used as the samples for Xpert MTB/RIF, LAMP and SAT-TB assay. Of these, 265 including 223 patients diagnosed with TBP and 42 non-TBP patients used as controls were analyzed. Results: The sensitivities of Xpert MTB/RIF (27.4%), LAMP (26.5%) and SAT-TB assay (32.3%) were significantly higher than that of pleural effusion smear (14.3%, X 2 = 20.65, P < 0.001), whereas they were much lower than expected for the analysis of pleural effusion samples. Both SAT-TB assay and Xpert MTB/RIF demonstrated high specificities (100%) and PPVs (100%), but the NPVs of all of the tests were < 22%. The area under ROC curve of pleural effusion smear, LAMP, Xpert MTB/RIF and SAT-TB assays was 0.524 (95% CI 0.431-0.617), 0.632 (95% CI 0.553-0.71), 0.637 (95% CI 0.56-0.714) and 0.673 (95% CI 0.6-0.745). SAT-TB assays had the highest AUC. Conclusion: Nucleic acid amplification tests are not the first choice in the diagnosis of tuberculous pleurisy. In this type of test, SAT-TB is recommended because of its low cost, relatively more accurate compared with the other two tests. This prospective study was approved by The Ethics Committee of the Shanghai Pulmonary Hospital (approval number: K19-148).

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“…Similar previous investigations of NAATs for detecting MTB in PE have also reported modest sensitivities (21)(22)(23)(24). (27). In a meta-analysis of 40 studies of NAATs for TBP, PAI et al reported that these tests had low sensitivities (43-77%), but high specificities (95%) (28).…”

Section: Discussionmentioning

confidence: 99%

Diagnostic performance of nucleic acid tests in tuberculous pleurisy

Xiao

Yan

Han

2019

Preprint

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Background: Tuberculous pleurisy (TBP) is the most common form of extrapulmonary tuberculosis (TB). However, rapid diagnostic methods with high accuracy for tuberculous pleurisy are urgently needed. In the present study, we evaluated the diagnostic accuracy of Xpert MTB/RIF, LAMP and SAT-TB assay with pleural fluids from from culture-positive TBP patients. Methods:We prospectively enrolled 300 patients with exudative pleural effusions used as the samples for Xpert MTB/RIF, LAMP and SAT-TB assay. Of these, 265 including 223 patients diagnosed with TP and 42 non-TB patients used as controls were analyzed. Results:The sensitivities of Xpert MTB/RIF (27.4%) , LAMP (26.5%) and SAT-TB assay (32.3%) were significantly higher than that of pleural effusion smear (14.3% , X2 = 20.65, P < 0.001), whereas they were much lower than expected for the analysis of pleural effusion samples. Both SAT-TB assay and Xpert MTB/RIF demonstrated high specificities (100%) and PPVs (100%), but the NPVs of all of the tests were < 22%. The area under ROC curve of pleural effusion smear, LAMP, Xpert MTB/RIF and SAT-TB assays was 0.524 (95% CI 0.431-0.617), 0.632 (95% CI 0.553-0.71), 0.637 (95% CI 0.56-0.714) and 0.673 (95% CI 0.6-0.745). SAT-TB assays had the highest AUC.Conclusion: Nucleic acid amplification tests are not the first choice in the diagnosis of tuberculous pleurisy. In this type of test, SAT-TB is recommended because of its low cost, relatively more accurate compared with the other two tests. This prospective study was approved by The Ethics Committee of the Shanghai Pulmonary Hospital (approval number: K19-148). ClinicalTrials.gov identifier:ChiCTR1900026234.

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Comparison of loop-mediated isothermal amplification and real-time PCR for the diagnosis of tuberculous pleurisy

Cited by 28 publications

References 25 publications

Diagnostic potential of multi‐targeted LAMP (loop‐mediated isothermal amplification) for osteoarticular tuberculosis

Diagnostic potential of multi‐targeted LAMP (loop‐mediated isothermal amplification) for osteoarticular tuberculosis

Diagnostic performance of nucleic acid tests in tuberculous pleurisy

Diagnostic performance of nucleic acid tests in tuberculous pleurisy

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